We narrowed to 50,226 results for: ELL

Showing: 9021 - 9040 of 50226 results

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pCMV-VSV-G-Myc

Plasmid

#80054

Purpose

Expression of a Myc tagged VSV-G protein

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

VSV-G-Myc

Tags

Myc

Expression

Mammalian

Available Since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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2a-copGFP(+HA)

Plasmid

#83806

Purpose

Express copGFP reporter for HDR-induced knock-in

Depositor

Bo Feng

Article

He et al Nucleic Acids Res. 2016 May 19;44(9):e85

Insert

copGFP

Use

Homologous recombination for gapdh loci

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pLH-stsgRNA2.1

Plasmid

#64117

Purpose

Vector for expression of St1 sgRNA2.1 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 sgRNA2.1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pKO2.2

Plasmid

#22676

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

DTA

Use

Mouse Targeting

Mutation

pDTA for targeting vector generation. I-CeuI lin…

Available Since

Dec. 4, 2009

Availability

Academic Institutions and Nonprofits only

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ph2b

Plasmid

#32109

Depositor

Christopher Buck

Article

Schowalter et al PLoS Pathog. 2011 Jul;7(7):e1002161. Epu

Insert

BKV VP2

Expression

Mammalian

Mutation

codon modified

Available Since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only

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JP1520 PIK3CA WT HA

Plasmid

#14570

Depositor

Joan Brugge

Article

Isakoff et al Cancer Res. 2005 Dec 1. 65(23):10992-100

Insert

PIK3CA (PIK3CA )

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only

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pRDA_112

Plasmid

#136475

Purpose

Lentiviral expression of AsCas12a

Depositor

John Doench

Article

DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

Insert

AsCas12a

Use

CRISPR and Lentiviral

Promoter

EF1a

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pTO_CHMP3_LAP

Plasmid

#101849

Purpose

Tet-inducible expression of human CHMP3 with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration

Depositor

Daniel Gerlich

Article

Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d

Insert

Charged Multivesicular Body Protein 3 (CHMP3 Human)

Use

Gateway cloning, flp-frt recombination, tet-induc…

Tags

Localization and Affinity Purification (LAP) tag

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pGL2-Mcl-1 mut

Plasmid

#19133

Depositor

Wafik El-Deiry

Article

Ricci et al Cancer Cell. 2007 Jul . 12(1):66-80.

Insert

Mcl-1 promoter (MCL1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

NFkB binding-site mutant, the sequence GGGGTCTTCC…

Available Since

Oct. 2, 2008

Availability

Academic Institutions and Nonprofits only

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pRL K-ras 3'UTR mutant

Plasmid

#14805

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

K-ras 3'UTR (Kras Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

let-7 miRNA binding site mutations

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-EGFR-V5/HIS

Plasmid

#201102

Purpose

expression of human EGFR receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human EGFR receptor tyrosine kinase, full length, wildtype (EGFR Human)

Tags

V5/His

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-PTDSS1-IRES-Puromycin

Plasmid

#134667

Purpose

Expresses EGFP-tagged Mitochondria-associated ER membrane marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

PTDSS1 (PTDSS1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-His:dCas9:nls:10P3

Plasmid

#135982

Purpose

Expresses dCas9 fused to 10 copies of the P3 coiled coil in mammalian cells

Depositor

Roman Jerala

Article

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Insert

dCas9 fused to 10 copies of the P3 coiled-coil

Use

CRISPR

Tags

His

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

CMV

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-VAMP5-IRES-Puromycin

Plasmid

#134662

Purpose

Expresses EGFP-tagged plasma membrane marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

vesicle-associated membrane protein 5 (VAMP5 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-Rab21-T31N (DN)

Plasmid

#83423

Purpose

Mouse Rab21 mutant that has defects in the binding of GTP (dominant negative). Generated from pEGFP-Rab21 by site-directed mutagenesis.

Depositor

Johanna Ivaska

Article

Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

Insert

Rab21 (Rab21 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Residue 31 from T to N (T31N: ACG -> AAC)

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pEYFP(C1)-PP1alpha

Plasmid

#44231

Depositor

Angus Lamond, Laura Trinkle-Mulcahy

Article

Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

Insert

PP1A (PPP1CA Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/HisC-mTAZ

Plasmid

#31793

Depositor

Michael Yaffe

Article

Hong et al Science. 2005 Aug 12;309(5737):1074-8.

Insert

TAZ (Wwtr1 Mouse)

Tags

EK (Enterokinase) Recognition Site, His, and Xpre…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pLNCX Myr His p110 KD

Plasmid

#10837

Depositor

Thomas Roberts

Article

Auger et al Biochem Biophys Res Commun. 2000 Jun 16.

Insert

p110 KD (PIK3CA Bovine)

Use

Retroviral

Tags

6xHis and Myr

Expression

Mammalian

Mutation

D915S. Kinase Dead.

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
Hygro-iDEST

Plasmid

#75339

Purpose

GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression

Depositor

Danwei Huangfu

Article

Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

Insert

TRE-DEST

Expression

Mammalian

Promoter

TRE-tight

Available Since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

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Galphai2-mTurquoise2

Plasmid

#69621

Purpose

The heterotrimeric G-protein subunit, Gαi isoform 2, tagged with mTurquoise2

Depositor

Dorus Gadella

Article

van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

Insert

Galphai2-mTurquoise2

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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