We narrowed to 10,569 results for: fus
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Plasmid#217684PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertCELSR1 (CELSR1 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-2447Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBstSK+IRES-V5-IPpoI (mutant)
Plasmid#206322PurposeA plasmid that facilitates production of mRNA encoding V5 epitope tagged catalytically dead I-PpoIDepositorInsertIRES element-V5 epitope tag-mutated IPpoI open reading frame
UseMrna productionTagsV5-IPpoI fusion protein (mutant version)Available SinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pASPIre4
Plasmid#196656PurposeDerivative of pASPIre3. Contains SpeI restriction site within the CDS of bxb1 to enable diversification of the 5’-UTR and codons 2-16.DepositorInsertBxb1-sfGFP fusion controlled by rhamnose promoter; attB/attP-flanked discriminator; exchangable 5'-UTR and CDS (codons 1-16)
ExpressionBacterialMutationSpeI site in Bxb1 CDSPromoterrhamnose-inducible promoterAvailable SinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL1P6 ZmUbiP:GRF-GIF:NosT
Plasmid#198049PurposeMoClo Golden Gate Level 1 Position 6. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.InsertZmUbiP::TaGRF4-GIF1::NosT
UseSynthetic BiologyPromoterZea mays (maize) ubiquitin promoterAvailable SinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRRL-pEF-H2B-mCherry-T2A-rTetR-DNMT3L-SV40
Plasmid#186967PurposeLentiviral vector encoding DNMT3A recruiter for methylation reporter (pEF-H2B-mCherry-T2A-rTetR-DNMT3L-SV40) for expression in mammalian cells.DepositorInsertDNMT3L (DNMT3L Human, Synthetic)
UseLentiviralTagsrTetR fusionExpressionMammalianPromoterEF-1alpha promoterAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ki67 (shuffled Nterm)-mNeonGreen-IRESpuro2
Plasmid#183744PurposeExpresses Ki67 (shuffled Nterm)-mNeonGreenDepositorInsertMKI67 (shuffled Nterm) (MKI67 Human)
TagsmNeonGreenExpressionBacterial and MammalianMutationCD domain with shuffled 4 fragments fused to the …PromoterCMVAvailable SinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-VAMP8
Plasmid#135553PurposeExpress mouse VAMP8 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertVAMP8
Tags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZIP-hCMV-ZsGreen-P2A-Puro-NT4
Plasmid#123328PurposeLentiviral vector for expression of non-targeting control shRNA #4 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insertDepositorInsertnon-targeting control shRNA embedded in miR30 backbone
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pNB784
Plasmid#60163PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by SIC1 promoter.DepositorInsertFLuc-yEVenus
ExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterSIC1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB788
Plasmid#60165PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by RNR1 promoter.DepositorInsertFLuc-yEVenus
ExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterRNR1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWZLblasti-HA-MEK1-MEK5DD
Plasmid#53177PurposeExpresses HA tagged chimeric human MEK1 (nucleotides 1–201)-MEK5 (498–1344 with S311DT315D) from blasticidin resistance retroviral vectorDepositorInsertMEK1 (1-201)-MEK5 (498–1344) fusion (MAP2K1 Human)
UseRetroviralTagsHAExpressionMammalianMutationMEK1 (1-201) MEK5(498–1344 with S311D/T315D)Available SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR P4-P1R-EYFP
Plasmid#48350PurposeContributes the coding sequence for EYFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertEYFP
UseGateway CloningMutationContains a Kozak sequence. Does not contain a sto…PromoternoneAvailable SinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHYRSF236
Plasmid#46377DepositorInsertNpuDnaE intein delta variant, inactive
TagsH6-Smt3ExpressionBacterialMutationC1A, C+1A, deleted T76, V97, D98, L100 from NpuDn…PromoterT7Available SinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
VCDstub-GFP
Plasmid#33122DepositorInsertPcdh-γA3 (Pcdhga3 Mouse)
TagsEGFPExpressionMammalianMutationContains a signal sequence fused to a very small …PromoterCMVAvailable SinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pA-CBh.TP-CNOT8-V5H6.MCh.Puro
Plasmid#209947PurposeIn mammalian cells expresses TP fused to a CNOT8 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.DepositorInsertsCCR4-NOT transcription complex subunit 8 (CNOT8 Human)
mCherry fluorescent protein fused to puromycin resistance marker
TagsTP (MS2 coat protein) and V5 epitope with H6 tagExpressionMammalianPromoterCBh and CMVAvailable SinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT-C34-L
Plasmid#223967PurposeAnti-MT-C34 light chain expression plasmid; to be used with the heavy chain plasmid, pMT-C34-L (Plasmid 223966), to make the antibody.DepositorInsertVariable domain of mouse anti-MT-C34 IgG1 light chain jointed to constant region of human IgG1 light chain
ExpressionMammalianPromoterCMVAvailable SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRV1 CTFdeltaTA-FLAG
Plasmid#217743PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRV1 (ADGRV1 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-5896Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB602-OEP7-mVenus
Plasmid#213864PurposeBinary vector for the expression of mVenus flanked with an N-terminal 50 amino acids of chloroplas outer envelope membrane transit peptide in plants.DepositorInsertchloroplast outer envelope membrane transit peptide fused mVenus (OEP7 Mustard Weed)
ExpressionBacterial and PlantAvailable SinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv-mos::NG
Plasmid#138958PurposeUsed to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila where the active site is converted to the mosquito sequence. Expression by using Gal4DepositorArticleInsertkkv (NEWENTRY Fly)
UseUas transgene vectorTagsNeon Green fused to C terminus of KkvExpressionInsectMutation8 bp that are in the region that encodes the cata…PromoterUASAvailable SinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only