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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,351 results for: multi

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Showing: 9081 - 9100 of 18351 results

  1. CALCRL-DuET

    Plasmid
    #213194
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CALCRL (CALCRL Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    213194-plasmid-map-sequence-id-431751

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  2. pVG122

    Plasmid
    #211765
    Purpose
    Expresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.
    Depositor
    Sahand Jamal Rahi
    Publication
    Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:
    Insert
    strongLOV
    Use
    For single-copy integration (if cut with pmei)
    Tags
    SV40 NLS and VP16
    Expression
    Yeast
    Mutation
    compared to EL222, strongLOV contains Glu84Asp mu…
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211765-plasmid-map-sequence-id-417945

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  8. pMZ-180

    Plasmid
    #195428
    Purpose
    Transient, constitutive expression of synZiFTR: ZF5-p65
    Depositor
    Ahmad Khalil
    Publication
    Li et al Science. 2022 Dec 16;378(6625):1227-1234
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pUb - 3XFLAG - NLS - ZF5-7 (ZF5) - p65 - RbGpA
    Expression
    Mammalian
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195428-plasmid-map-sequence-id-385002

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  9. pSBK.134

    Plasmid
    #187219
    Purpose
    Expresses Eco1 ncRNA v35 (long a1/a2) and Eco1 ncRNA v35 (long a1/a2, barcode 6) from promoters pTet* and pBetI, respectively
    Depositor
    Seth Shipman
    Publication
    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:
    Insert
    A: Retron Eco1 ncRNA v35 (long a1/a2); B: Retron Eco1 ncRNA v35 (long a1/a2, barcode 6)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    A: pTet*; B: pBetI
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    187219-plasmid-map-sequence-id-367486

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  12. pMC_mClover3_BSDminus_F2

    Plasmid
    #184167
    Purpose
    Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.
    Depositor
    Ophir Shalem
    Publication
    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    mClover3
    bsd
    Use
    CRISPR
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    184167-plasmid-map-sequence-id-360739

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  13. pMC_mClover3_BSDminus_F1

    Plasmid
    #184166
    Purpose
    Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.
    Depositor
    Ophir Shalem
    Publication
    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    mClover3
    bsd
    Use
    CRISPR
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    184166-plasmid-map-sequence-id-360737

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  15. pCarCBA

    Plasmid
    #117754
    Purpose
    Codon optimized CarCBA enzymes from Pectobacterium carotovorum in pBbaA5k backbone
    Depositor
    Greg Bokinsky
    Publication
    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d
    Insert
    Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA)
    Expression
    Bacterial
    Promoter
    PLac(UV5)
    Available since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    117754-plasmid-map-sequence-id-229023

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  17. [GFP][IRES][Bla]

    Plasmid
    #97194
    Purpose
    Vector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.
    Depositor
    Edward Boyden
    Publication
    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    beta-Lactamase
    GFP
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    97194-plasmid-map-sequence-id-197878

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  18. pGAPTrap-eGFP-IRESMygro

    Plasmid
    #82503
    Purpose
    Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. Hygromycin B resistance is encoded by the Mygro gene.
    Depositor
    Ed Stanley
    Publication
    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eGFP-IRESMygro
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    82503-plasmid-map-sequence-id-161768

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Showing: 9081 - 9100 of 18351 results