We narrowed to 23,538 results for: Sis;

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Halo-WIPI2 HRD

Plasmid

#207544

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous WIPI2 locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag flanked by human WIPI2 locus sequences

Tags

HaloTag

Expression

Mammalian

Promoter

Endogenous

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pBabe hygro NFS1

Plasmid

#102977

Purpose

Expression of NFS1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

NFS1 (NFS1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Silent mutations to eliminate shNFS1_1 binding si…

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pMaCTag-26

Plasmid

#120005

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mAID-Clover

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mAID-Clover

Use

Pcr template

Tags

mAID-Clover

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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HBT_pcoCASphi_version2

Plasmid

#197932

Purpose

Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the C-terminal

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Insert

pcoCasphi-2

Use

CRISPR

Tags

2xNLS-2xFLAG

Expression

Plant

Promoter

35SPPDK promoter

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAC-CR7T-gRNA2.1-nlsBFP

Plasmid

#170515

Purpose

gRNA cloning vector containing a CR7T promoter, gRNA(2.1) scaffold, and a Ubi-mTagBFP-NLS marker; optimized for germline CRISPR.

Depositor

Chun Han

Article

Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

Type

Empty backbone

Use

CRISPR

Expression

Insect

Promoter

CR7T

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST_6xUAS:miRFP670-cetn4

Plasmid

#192357

Purpose

zfCETN4 tagged with miRFP670, expressed under the UAS promoter

Depositor

Darren Gilmour

Article

Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

Insert

CETN4 (cetn4 Zebrafish)

Tags

miRFP670

Expression

Mammalian

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-Pidd1(T788A)

Plasmid

#60529

Purpose

Retroviral expression of Pidd1 T788A phosphorylation mutant

Depositor

Trudy Oliver

Article

Terry et al Cell Death Differ. 2014 Oct 10. doi: 10.

Insert

Pidd1 (PIDD1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Threonine 788 to Alanine*

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only
TagRFP-T-Rabenosyn-5

Plasmid

#37537

Depositor

Silvia Corvera

Article

Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;10

Insert

Rabenosyn5 (RBSN Human)

Tags

TagRFP-T

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2013

Availability

Academic Institutions and Nonprofits only

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eGFP-CBP

Plasmid

#169211

Purpose

Expression of eGFP-CBP protein for chitin visualization

Depositor

James S Fraser

Article

Ricardo-Gonzalez et al bioRxiv 2021.03.29.437438

Insert

Chitinase A1 Chitin Binding Domain

Tags

6xHis, Mxe Gyr Intein, and eGFP

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Ost4-GFP-MTn

Plasmid

#154351

Purpose

Mammalian expression of MTn

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

MTn

Tags

GFP

Expression

Mammalian

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pEF1α-squirrel-monkey-retroCHMP3-HA

Plasmid

#154174

Purpose

expresses squirrel monkey retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

retroCHMP3

Tags

HA

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-TRAF6-C70A

Plasmid

#43930

Depositor

Bryant Darnay

Article

Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

Insert

TNF Receptor-Associated Factor 6 (Traf6 )

Tags

GST

Expression

Bacterial

Mutation

C70A

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pSin wPGK-Cre

Plasmid

#101242

Purpose

low-efficiency Cre used to turn on dio YFP/ PSD95-mCherry/ Teal-gephyrin expression

Depositor

Elly Nedivi

Article

Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

Insert

Cre recombinase

Use

Lentiviral

Mutation

see attached file

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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Clathrin-LCa-ECFP

Plasmid

#21742

Depositor

Xiaowei Zhuang

Article

Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

Insert

Clathrin, light polypeptide (LCa) (Clta Mouse)

Tags

ECFP

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
pET-BoNT/E

Plasmid

#168020

Purpose

Expresses BoNT/E-LC(1-411)-6xHis in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

BoNT/E-LC(1-411)-6xHis

Tags

Hisx6

Expression

Bacterial

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pnxABE-flgn

Plasmid

#187932

Purpose

A-to-G base editor

Depositor

Yi Xiao

Article

et al ACS Synth Biol. 2022 Apr 15;11(4):1650-1

Insert

ecTadA(8e)-nxCas9 (D10A)

Use

CRISPR

Expression

Bacterial

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-eA3Amax-T31A-NG-BlastR

Plasmid

#153000

Purpose

C-to-T/G/A base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

eA3A-BE4 T31A-nSpCas9-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCS-H2B-citrine

Plasmid

#53752

Purpose

Contains fusion of human Histone H2B to citrine.

Depositor

Sean Megason

Article

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Insert

histone H2B (H2BC21 Human)

Tags

citrine

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

July 8, 2014

Availability

Academic Institutions and Nonprofits only

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pUPD2_miniDFR (GB2566)

Plasmid

#160595

Purpose

Minimal promoter of slDFR gene containing 62bp upstream the transcription start site and the 5'UTR region

Depositor

Diego Orzaez

Article

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

miniDFR

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pMOD_C'5802

Plasmid

#176879

Purpose

High constitutive expression of Firefly Luciferase in plants

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

AtUbi10:Luc+:RbcsE9

Use

Luciferase

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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