We narrowed to 9,809 results for: crispr plasmids

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px458-CCND1

Plasmid

#172630

Purpose

Expresses a gRNA against CCND1 and Cas9 from S. pyogenes with 2A-EGFP

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

gRNA against CCND1 (CCND1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-SaKKH-spA-miniU6-miniSdd6-MmHPD-T2

Plasmid

#204852

Purpose

AAV vector for cytosine base editing using miniSdd6 at HPD-T2 target site in mouse

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

miniSdd6-nSaCas9KKH(D10A)-UGI

Use

AAV and CRISPR

Mutation

D10A, E782K, N968K, R1015H in SaCas9

Promoter

EFS, miniU6

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pUC57-2xFLAG-mAID-AMBRA1 HDR donor template

Plasmid

#172609

Purpose

Donor plasmid containing the HDR template for the in-frame, N-terminal 2xFLAG-mAID tagging of AMBRA1 gene

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

2xFLAG-mAID-AMBRA1 (AMBRA1 Human, Synthetic)

Use

CRISPR; Human targeting

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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LCV2_mClover3_AAVS1_sgRNA_2

Plasmid

#155099

Purpose

lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ284-v2

Plasmid

#138119

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with D172R, N563R and K569R mutations, without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

Mb2Cas12a v2

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Mutation

D172R, N563R and K569R

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pOTTC1440 - pAAV CMV-IE Nuc-iRFP-2A-Flpo

Plasmid

#112684

Purpose

An AAV vector that expresses a nuclear-localized iRFP713 reporter and optimized Flp recombinase

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

iRFP713

Use

AAV

Tags

2A-Flpo and Nuclear localization signal

Promoter

CMV-IE

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-ACSL3-sfGFP(C) HDR template

Plasmid

#129413

Purpose

HDR tempalte for tagging of endogenous human ACSL3 C-terminus with sfGFP

Depositor

Elina Ikonen

Article

Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

Insert

ACSL3 HDR template (ACSL3 Human)

Use

CRISPR and TALEN; Endogenous tagging hdr template

Tags

sfGFP

Expression

Mammalian

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ284-RVR

Plasmid

#138117

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with N563R, K569V and N573R mutations, without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

Mb2Cas12a RVR variant

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Mutation

N563R, K569V and N573R

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ289-RVR

Plasmid

#138127

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized ErCas12a with D513R, K519V and N523R mutations, without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

ErCas12a RVR variant

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Mutation

D513R, K519V and N523R

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ284-v1

Plasmid

#138118

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with D172R mutation, without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

Mb2Cas12a v1

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Mutation

D172R

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-crRNA(SOD1)

Plasmid

#109321

Purpose

AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human SOD1

Depositor

Feng Zhang

Article

Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

Insert

mCherry-KASH (SOD1 Human)

Use

AAV

Promoter

hSyn1

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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LCV2_mCherry_PSMD1_sgRNA_1

Plasmid

#155107

Purpose

lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting PSMD1 (core essential gene)

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

PSMD1_sgRNA_1 (PSMD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ223-STU

Plasmid

#138109

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized, catalytically deactivated AsCas12a-SRDX repressor without promoter and terminator

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

dAsCas12a-SRDX

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-CD16-TLR4-HA

Plasmid

#246906

Purpose

Expresses CD16-TLR4 chimera in mammalian cells

Depositor

William Kaelin

Article

Lampson et al Cell. 2024 Apr 25;187(9):2209-2223.e16.

Insert

CD16-TLR4 (TLR4, FCGR3B Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

N terminus of CD16, C terminus of TLR4

Promoter

CMV

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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rTTA-gRNA-AAV

Plasmid

#213036

Purpose

This vector contains rTTA expressed under CMV promoter and gRNA expressed under U6 promoter

Depositor

Nadav Ahituv

Article

Nguyen et al Nat Biotechnol. 2025 Feb 4:10.1038/s4158

Insert

rTTA-T2A-mCherry

Use

AAV and CRISPR

Promoter

CMV

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pTR-454 tNGFR-P2A-CARD11

Plasmid

#186081

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CARD11 (CARD11 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-NLS(SV40)-anCas(FCA)-6xHis-NLS(SV40)

Plasmid

#185701

Purpose

CMV and T7 promoter expression plasmid for human codon usage anCas(FCA) with an N-terminal NLS and C-terminal NLS

Depositor

Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

anCas(FCA)

Tags

6xHis-NLS and NLS

Expression

Mammalian

Mutation

optimized for human codon usage

Promoter

CMV and T7

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-NLS(SV40)-anCas(PDCA)-6xHis-NLS(SV40)

Plasmid

#185705

Purpose

CMV and T7 promoter expression plasmid for human codon usage anCas(PDCA) with an N-terminal NLS and C-terminal NLS

Depositor

Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

anCas(PDCA)

Tags

6xHis-NLS and NLS

Expression

Mammalian

Mutation

optimized for human codon usage

Promoter

CMV and T7

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-NLS(SV40)-anCas(SCA)-6xHis-NLS(SV40)

Plasmid

#185703

Purpose

CMV and T7 promoter expression plasmid for human codon usage anCas(SCA) with an N-terminal NLS and C-terminal NLS

Depositor

Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

anCas(SCA)

Tags

6xHis-NLS and NLS

Expression

Mammalian

Mutation

optimized for human codon usage

Promoter

CMV and T7

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-NLS(SV40)-anCas(PCA)-6xHis-NLS(SV40)

Plasmid

#185704

Purpose

CMV and T7 promoter expression plasmid for human codon usage anCas(PCA) with an N-terminal NLS and C-terminal NLS

Depositor

Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

anCas(PCA)

Tags

6xHis-NLS and NLS

Expression

Mammalian

Mutation

optimized for human codon usage

Promoter

CMV and T7

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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