We narrowed to 7,300 results for: RAP

1. pcs2-cDELE1(142-515)-sumo

   Plasmid

   #226107

   Purpose

   cDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) fused to sumo used for in-vitro translation by wheat germ extract in in-vitro ubiquitylation assays

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   DELE1 (DELE1 Human)
   

   Use

   Tags

   Sumo

   Expression

   Mammalian

   Mutation

   encodes only residues 142-515 of DELE1 (represent…

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcs2-MTS-cox8A-GFP-IRES-mCherry

   Plasmid

   #226103

   Purpose

   Stability reporter construct of the COX8A mitochondrial targeting sequence (MTS) for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   COX8A (COX8A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   encodes only for mitochondrial targeting sequence…

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcs2-cDELE1(142-515)-GFP-IRES-mCherry

   Plasmid

   #226102

   Purpose

   cDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) stability reporter construct for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   DELE1 (DELE1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   only encodes for residues 142-515 of DELE1 corres…

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcs2-ATF4uORF1-2-GFP-IRES-mCherry

   Plasmid

   #226105

   Purpose

   ATF4uORF1-2 stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells to read out activation of the integrated stress response (ISR)

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   atf4 (ATF4 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains uORF1-2 of ATF4, whose stability can ser…

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS L30 Flag-Apex-Rab7A

   Plasmid

   #135652

   Purpose

   Rab7A fused to APEX2 and a Flag tag in N-ter under control of a weak L30 promoter.

   Depositor

   Raphael Gaudin

   Article

   Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7

   Insert

   Rab7A (RAB7A Human)
   

   Use

   Tags

   Apex2 and FLag

   Expression

   Mammalian

   Mutation

   Promoter

   L30

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2+ GFP-CDK5R1 degron (283-307)-IRES-mCherry

   Plasmid

   #231008

   Purpose

   Protein stability reporter construct for CDK5R1 consisting of aa 283-307 for transient overexpression in mammalian cells.

   Depositor

   Michael Rape

   Article

   Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

   Insert

   CDK5R1 aa283-307 (CDK5R1 Human)
   

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2-MYC-GFP-IRES-mCherry

   Plasmid

   #231232

   Purpose

   MYC stability reporter construct (expressing c-myc 2) for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

   Depositor

   Michael Rape

   Article

   Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

   Insert

   MYC (MYC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2-MCRS1-GFP-IRES-mCherry

   Plasmid

   #231231

   Purpose

   MCRS1 stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

   Depositor

   Michael Rape

   Article

   Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

   Insert

   MCRS1 (MCRS1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti gRNA KO OCLNx2 spCas9-T2A-iRFP670-P2A-puro

   Plasmid

   #208399

   Purpose

   codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, iRFP670 fluorescent protein and puromycin resistance

   Depositor

   Raphael Gaudin

   Article

   Baptiste Pradel et al bioRxiv

   Insert

   hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti gRNA KO OCLNx2 spCas9-T2A-Crimson-P2A-puro

    Plasmid

    #208398

    Purpose

    codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, E2-Crimson fluorescent protein and puromycin resistance

    Depositor

    Raphael Gaudin

    Article

    Baptiste Pradel et al bioRxiv

    Insert

    hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    E2-Crimson

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti gRNA KO OCLNx2 spCas9-T2A-mNeonGreen-P2A-puro

    Plasmid

    #208400

    Purpose

    codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, mNeonGreen fluorescent protein and puromycin resistance

    Depositor

    Raphael Gaudin

    Article

    Baptiste Pradel et al bioRxiv

    Insert

    hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410)

    Plasmid

    #123615

    Purpose

    CAG promoter expression plasmid for rAPOBEC1(R33A/K34A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A/K34A).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

    Insert

    BE3(R33A/K34A)-P2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A/K34A in rAPOBEC1, D10A in SpCas9

    Promoter

    CAG

    Available since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. SECURE BE3(R33A)-P2A-EGFP (pJUL1085)

    Plasmid

    #123614

    Purpose

    CAG promoter expression plasmid for rAPOBEC1(R33A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

    Insert

    BE3(R33A)-P2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, D10A in SpCas9

    Promoter

    CAG

    Available since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKLD-CAG-DYSF-CFL

    Plasmid

    #131457

    Purpose

    Encodes Dysferlin protein (Therapeutic gene) along with Follistatin protein to cure LGMD2B along with luciferase enzyme for in vivo live imaging

    Depositor

    Michele Calos

    Article

    Guha et al Mol Ther Methods Clin Dev. 2019 Oct 14;1

    Insert

    DYSF and Follistatin (DYSF, FST Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    R15C (in DYSF) and Q2579H (in CFL)

    Promoter

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. BE3(E63Q)-P2A-EGFP (pRZ189)

    Plasmid

    #123613

    Purpose

    CAG promoter expression plasmid for rAPOBEC1(E63Q)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (catalytically impaired BE3 mutant).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

    Insert

    BE3(E63Q)-P2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E63Q in rAPOBEC1, D10A in SpCas9

    Promoter

    CAG

    Available since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2-MYC(Δ1-143)-GFP-IRES-mCherry (ΔTAD)

    Plasmid

    #231233

    Purpose

    MYC stability reporter construct with deletion of the N-terminal region (residues 1-143) for transient expression in mammalian cells

    Depositor

    Michael Rape

    Article

    Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

    Insert

    MYC (MYC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    TAD deleted (delta1-143); numbering based on sequ…

    Promoter

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2-HA-MycΔdeg1∆deg2

    Plasmid

    #231243

    Purpose

    HA-tagged MYC over-expession construct with point mutations corresponding to the deletion of degron 1 and 2 for transient expression in mammalian cells

    Depositor

    Michael Rape

    Article

    Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

    Insert

    MYC (MYC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of MYC degrons 1 & 2 (K355E; R356E; …

    Promoter

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFastBac Dual His6-TEV-CUL2, Rbx1

    Plasmid

    #231000

    Purpose

    Dual expression vector containing mouse CUL2 and Rbx1 for protein puritification from SF9 insect cells

    Depositor

    Michael Rape

    Article

    Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

    Inserts

    Rbx1 (Rbx1 Mouse)
    CUL2 (Cul2 Mouse)
    

    Use

    Tags

    6x HIS

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcs2-MTSmut-cox8A(XXXX)-GFP-IRES-mCherry

    Plasmid

    #226104

    Purpose

    Stability reporter construct of the COX8A mitochondrial targeting sequence (MTS) with mutations that impair recognition by SIFI for transient expression in mammalian cells

    Depositor

    Michael Rape

    Article

    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

    Insert

    COX8A (COX8A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    encodes only for mitochondrial targeting sequence…

    Promoter

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. His6-TEV-CRBN-midi

    Plasmid

    #215330

    Purpose

    CRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader design

    Depositor

    Alessio Ciulli

    Article

    Kroupova et al Nat Commun. 2024 Oct 15;15(1):8885. doi:

    Insert

    CRBN (CRBN Human)
    

    Use

    Tags

    6xHis and TEV

    Expression

    Bacterial

    Mutation

    contains amino acids 41-187 followed by a Gly-Ser…

    Promoter

    lacI

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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