We narrowed to 23,737 results for: nts

1. GFP-AuroraB L154G/H250Y

   Plasmid

   #108492

   Purpose

   expression of EGFP-AuroraB Analog sensitive (L154G/H250Y)

   Depositor

   Susanne Lens

   Article

   Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

   Insert

   AuroraB (AURKB Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Analog sensitive L154G/H250Y

   Promoter

   CMV

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCR3-Flag-AuroraB KD (K106R)

   Plasmid

   #108488

   Purpose

   expression of Flag-AuroraB kinase death (K106R)

   Depositor

   Susanne Lens

   Article

   Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

   Insert

   AuroraB (AURKB Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Kinase death K106A

   Promoter

   CMV

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJW1759

   Plasmid

   #154315

   Purpose

   NT Donor-cassette for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker::TEV::AID*::3xFLAG for NT slot
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. RCAS-sgATG7

   Plasmid

   #75228

   Purpose

   An adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor Gammoh

   Depositor

   Noor Gammoh

   Article

   Fraser et al EMBO Rep. 2019 Oct 4;20(10):e47734. doi:

   Insert

   Autophagy Related 7 (Atg7 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   June 2, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pHR-mCherry-p2A-LIC1 C-terminal half

   Plasmid

   #74603

   Purpose

   expresses LIC1 in mammalian cells; aa 389-523, untagged but cells indicate expression with diffuse mCherry

   Depositor

   Ron Vale

   Article

   Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

   Insert

   full length human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
   

   Use

   Lentiviral

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   amino acids 389-523 of LIC1

   Promoter

   SFFV

   Available since

   May 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. FUW TetO NET1A-V5 WT

   Plasmid

   #69828

   Purpose

   Expresses NET1A-V5 in mammalian cells in a doxycycline inducible manner

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)
   

   Use

   Lentiviral

   Tags

   v5

   Expression

   Mammalian

   Mutation

   Promoter

   minimal CMV promoter with tetracycline operator

   Available since

   April 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pECE M2-SH3PXD2A 8 serines mutated to alanines

   Plasmid

   #69816

   Purpose

   Expresses M2-SH3PXD2A in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   SH3 and PX domain-containing protein 2A (SH3PXD2A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   SV40 early promoter

   Available since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHR-mCherry-p2A-LIC1 G domain

   Plasmid

   #74602

   Purpose

   expresses LIC1 in mammalian cells; aa 1-389, untagged but cells indicate expression with diffuse mCherry

   Depositor

   Ron Vale

   Article

   Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

   Insert

   full length human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)
   

   Use

   Lentiviral

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   May 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJW1806

   Plasmid

   #154319

   Purpose

   NT Donor-cassette for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   linker::3xMyc for NT slot
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJW1787

    Plasmid

    #154316

    Purpose

    NT Donor-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    linker::TEV::BioTag::AID*::3xFLAG donor for NT slot in the SapTrap cloning system
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJW1348

    Plasmid

    #154311

    Purpose

    NT Donor-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker for NT slot
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pECE M2-SH3PXD2A 6 serines mutated to alanines

    Plasmid

    #69815

    Purpose

    Expresses M2-SH3PXD2A in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    SH3 and PX domain-containing protein 2A (SH3PXD2A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SV40 early promoter

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pECE M2-SH3PXD2A S974A

    Plasmid

    #69814

    Purpose

    Expresses M2-SH3PXD2A in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    SH3 and PX domain-containing protein 2A (SH3PXD2A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SV40 early promoter

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA 3.2/V5-DEST NET1A S46A

    Plasmid

    #69818

    Purpose

    Expresses NET1A-V5 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    S46A

    Promoter

    CMV

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCR3-vsv-AuroraB L154G/H250Y

    Plasmid

    #108490

    Purpose

    expression of vsv-AuroraB Analog sensitive (L154G/H250Y)

    Depositor

    Susanne Lens

    Article

    Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

    Insert

    AuroraB (AURKB Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    Analog sensitive L154G/H250Y

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FUW TetO NET1A-V5 S46A

    Plasmid

    #69829

    Purpose

    Expresses NET1A-V5 in mammalian cells in a doxycycline inducible manner

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)
    

    Use

    Lentiviral

    Tags

    v5

    Expression

    Mammalian

    Mutation

    S46A

    Promoter

    minimal CMV promoter with tetracycline operator

    Available since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX6P1-human LIC1 G domain

    Plasmid

    #74598

    Purpose

    used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 1-389

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)
    

    Use

    Tags

    N-terminal GST and Strep II

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5/FRT/TO-3xFLAG-His6-LARP1-d327-496

    Plasmid

    #233627

    Purpose

    Expression of Flag- and His-tagged LARP1delta327-496

    Depositor

    Shintaro Iwasaki

    Article

    Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

    Insert

    LARP1delta327-496 (LARP1 Human)
    

    Use

    Tags

    3FLAG-His6

    Expression

    Mammalian

    Mutation

    delta327-496

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYO379

    Plasmid

    #235733

    Purpose

    Protein expression of VPS34 + VPS15-ZZ + ZZ-VPS30 (all yeast) in yeast

    Depositor

    Roger Williams

    Article

    Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

    Inserts

    VPS34 (VPS34 Budding Yeast)
    VPS15 (VPS15 Budding Yeast)
    VPS30 (VPS30 Budding Yeast)
    

    Use

    Tags

    3xTEV-ZZ and ZZ-3xTEV

    Expression

    Yeast

    Mutation

    S2A on ScVPS34

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pColdI-SBP-DDX3_helicase_core

    Plasmid

    #233622

    Purpose

    Expression of SBP-tagged helicase core region of DDX3X in E.coli

    Depositor

    Shintaro Iwasaki

    Article

    Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

    Insert

    SBP-DDX3X helicase core (EIF4A1 Human)
    

    Use

    Tags

    His6-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge