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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 6,524 results for: poly

Showing: 901 - 920 of 6524 results

  2. PARP1-c009

    Plasmid
    #216872
    Purpose
    Bacterial expression vector for E. coli producing PARP1 catalytic protein (without Regulatory domain); with N-terminal CFP fusion
    Depositor
    Lari Lehtiö
    Article
    Dhakar et al Sci Rep. 2024 Feb 16;14(1):3875. doi: 10
    Insert
    PARP1 catalytic domain (no Regulatory Domain) (PARP1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216872-plasmid-map-sequence-id-434365

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  3. (SIM)10

    Plasmid
    #126946
    Purpose
    Bacterial expression plasmid for (SIM)10 client to assess SUMO-SIM scaffolds.
    Depositor
    Michael Rosen
    Article
    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1
    Insert
    10 repeats of SUMO interacting motif (SIM) from PIASx each separated by (GGS)4 (PIAS2 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    unknown
    Available since
    Sept. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    126946-plasmid-map-sequence-id-256126

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  6. pYFP-NEAT1m_v1-RT

    Plasmid
    #97089
    Purpose
    Encodes a HR repair template for knock-in a YFP expression cassette downstream of the poly(A) signal of human NEAT1_1 isoform. Best used with px330-NEAT1m_v1 vector.
    Depositor
    Archa Fox
    Article
    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d
    Insert
    NEAT1m_v1-RT (NEAT1 Human)
    Use
    CRISPR
    Tags
    EYFP
    Expression
    Mutation
    Promoter
    CMV
    Available since
    July 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    97089-plasmid-map-sequence-id-189689

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  7. pYFP-NEAT1_IS_RT

    Plasmid
    #97091
    Purpose
    Encodes a HR repair template that mutates the poly(A) signal as well as a few splicing factor binding sites of human NEAT1_1 isoform. Best used with px335-NEAT1_IS_v1 and v2 vectors.
    Depositor
    Archa Fox
    Article
    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d
    Insert
    NEAT1_IS_RT (NEAT1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    July 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    97091-plasmid-map-sequence-id-189691

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  9. pUWR-CadTS

    Plasmid
    #58325
    Purpose
    Destination vector for inserting ubi-CadTS to Drosophila melanogaster genome
    Depositor
    Denise Montell
    Article
    Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1
    Insert
    E-cadherin tension sensor
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    poly-ubiquitin
    Available since
    Aug. 26, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. pCAGGS-C-TEV-HAT

    Plasmid
    #202597
    Purpose
    Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Type
    Empty backbone
    Use
    Tags
    TEV cleavable site (ENLYFQG) and poly-histidine a…
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202597-plasmid-map-sequence-id-399552

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  12. pCAGGS-N-TEV-HAT

    Plasmid
    #202598
    Purpose
    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Type
    Empty backbone
    Use
    Tags
    TEV cleavable site (ENLYFQG) and poly-histidine a…
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202598-plasmid-map-sequence-id-399468

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  15. pTK331

    Plasmid
    #59572
    Purpose
    TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceI
    Depositor
    Ron Weiss
    Article
    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038
    Insert
    SGP(16) 2xK-turn Kozak EGFP attB2 AscI
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    59572-plasmid-map-sequence-id-92977

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  16. pG2J

    Plasmid
    #102882
    Purpose
    Expresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.
    Depositor
    Matthias Heinemann
    Article
    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi
    Inserts
    OsTIR1
    mGFP-AID
    Use
    Tags
    AID(71-114)
    Expression
    Yeast
    Mutation
    Promoter
    ADH1 and TEF1
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    102882-plasmid-map-sequence-id-199289

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  17. pUWR-CadTS-C

    Plasmid
    #58326
    Purpose
    Destination vector for inserting ubi-CadTS control to Drosophila melanogaster genome
    Depositor
    Denise Montell
    Article
    Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1
    Insert
    E-cadherin tension sensor control
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    poly-ubiquitin
    Available since
    Aug. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 901 - 920 of 6524 results