We narrowed to 6,820 results for: poly

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pT3TS-HiFi-nCas9n-A72

Plasmid

#125646

Purpose

micro-injection of poly-adenylated HiFi-nCas9n RNA

Depositor

Jason Berman

Article

Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

Insert

HiFi-nCas9n

Use

Rna in vitro transcription vector

Mutation

R691A from wild-type Cas9

Promoter

T3

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pSB_init_Sb92

Plasmid

#159424

Purpose

Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb92

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

Sb92

Tags

c-Myc and poly-His

Expression

Bacterial

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pSB_init_Sb68

Plasmid

#159423

Purpose

Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb68

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

Sb68

Tags

c-Myc and poly-His

Expression

Bacterial

Available Since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pYTSG

Plasmid

#159419

Purpose

Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Saccharomyces cerevisiae

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

thermostable GFP (TGP)

Tags

polyhistidine (poly-His)

Expression

Yeast

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pSB_init-Sb66

Plasmid

#159422

Purpose

Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb66

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

Sb66

Tags

c-Myc and poly-His

Expression

Bacterial

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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Pub-mCD8:GFP-T2A-DsRed:NLS-SV40

Plasmid

#130665

Purpose

Plasmid to test the efficiency of T2A multiplexing in Aedes aegypti

Depositor

Leslie Vosshall

Article

Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

Insert

AaegPUb-mCD8-GFP-T2A-dsRed-NLS-SV40

Use

Ae. aegypti expression

Expression

Insect

Promoter

Aedes aegypti poly-ubiquitin

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pHIT239

Plasmid

#204522

Purpose

In vitro transcription of Mos1 transposase mRNA with SL1 and poly(A) used for microinjection into nematode

Depositor

Yuji Kohara

Article

Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

Insert

Mos1_transposase::glh-2_3'UTR (glh-2 Nematode)

Use

Other

Promoter

T7 (minimum seq.)

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pJEP206-AAV-1.3CaMKII-MCS-pA.

Plasmid

#78319

Purpose

AAV vector backbone containing a 1.3CaMKIIa promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Type

Empty backbone

Use

AAV

Promoter

1.3CaMKIIa

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP210-AAV-0.4CaMKII P-MCS-pA

Plasmid

#63084

Purpose

AAV2 vector backbone containing a 0.4CaMKIIa promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Type

Empty backbone

Use

AAV

Promoter

0.4αCaMKII

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP222-AAV-CMV-GFP-pA

Plasmid

#62916

Purpose

AAV vector backbone designed to express GFP from a CMV promoter. It also contains a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

AAV

Promoter

CMV

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP220-AAV-0.5Synapsin-GFP-pA

Plasmid

#62920

Purpose

AAV vector backbone designed to express GFP from a 0.5Synapsin promoter. It also contains a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

AAV

Promoter

0.5synapsin

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP219-AAV-1.1Synapsin-GFP-pA

Plasmid

#62921

Purpose

AAV vector backbone designed to express GFP from a 1.1Synapsin promoter. It also contains a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

AAV

Promoter

1.1Synapsin

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-cGFP-SV40 pA + Laccase2 Exon 2

Plasmid

#91802

Purpose

Expresses the Laccase2 circular RNA when the upstream SV40 poly(A) signal fails to be used

Depositor

Jeremy Wilusz

Article

Liang et al Methods. 2021 Apr 18. pii: S1046-2023(21

Insert

Coral Green Fluorescent Protein (cGFP)

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-dSa-VPR mini.-1X snRP1

Plasmid

#99687

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 17 nt snRP1 poly adenylation signal

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VPR mini

Mutation

dead Cas9

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pHM6-Q74

Plasmid

#40264

Depositor

David Rubinsztein

Article

Narain et al J Med Genet. 1999 Oct;36(10):739-46.

Insert

HTT partial exon 1 Q74 (HTT Human)

Tags

6x His and HA

Expression

Mammalian

Mutation

expanded poly-Q

Promoter

CMV

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pHM6-Q23

Plasmid

#40263

Depositor

David Rubinsztein

Article

Narain et al J Med Genet. 1999 Oct;36(10):739-46.

Insert

HTT partial exon 1 Q23 (HTT Human)

Tags

6x His and HA

Expression

Mammalian

Mutation

expanded poly-Q

Promoter

CMV

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag1-NS5A

Plasmid

#17646

Depositor

Xin Wang

Article

Budhu et al Carcinogenesis. 2007 Jul . 28(7):1552-60

Insert

HCV NS5A (POLY Hepatitis C virus)

Tags

Flag

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag1-NS3

Plasmid

#17645

Depositor

Xin Wang

Article

Budhu et al Carcinogenesis. 2007 Jul . 28(7):1552-60

Insert

HCV NS3 (POLY Hepatitis C virus)

Tags

Flag

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-dSa-VPR mini.-syn pA

Plasmid

#99689

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains with syn pA (poly adenylation) signal

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VPR mini

Mutation

dead Cas9

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pTK312

Plasmid

#59569

Purpose

TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak EGFP ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP XbaI attB1 Kozak EGFP ochre attB2 AscI

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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