We narrowed to 6,637 results for: poly

1. pG23A

   Plasmid

   #102884

   Purpose

   Plasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.

   Depositor

   Matthias Heinemann

   Article

   Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

   Insert

   Cdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)
   

   Use

   Tags

   AID(71-114) and mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Native promoter

   Available since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pK RagA(Q66L)-RagC

   Plasmid

   #99700

   Purpose

   Bacterial expression of RagA(Q66L)-RagC

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Use

   Tags

   His tag, Poly Arg, and SUMO

   Expression

   Bacterial

   Mutation

   L91P

   Promoter

   T7

   Available since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJEP223-AAV-EFS-GFP-pA

   Plasmid

   #62913

   Purpose

   AAV vector backbone designed to express GFP from a EFS promoter. It also contains a poly-Adenylation Signal (pA)

   Depositor

   Jonathan Ploski

   Article

   Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

   Insert

   Green Fluorescent protein (GFP)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   short form of Elongation Factor1α (EFS)

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pK RagA-RagC

   Plasmid

   #99649

   Purpose

   Bacterial expression of wildtype Rag GTPase dimer

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Use

   Tags

   10x His tag, Poly arginine linker, and SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBT100-neo

   Plasmid

   #123137

   Purpose

   Golden Gate donor vector for the assembly of EGFP-based mammalian recombinase reporters.

   Depositor

   David Liu

   Article

   Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

   Insert

   Neomycin, poly-A terminator
   

   Use

   Synthetic Biology; Golden gate donor vector

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CMV-dSa-VPR mini.-1X snRP1

   Plasmid

   #99687

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 17 nt snRP1 poly adenylation signal

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Use

   AAV

   Tags

   VPR mini

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pK RagA-RagC(Q120L)

   Plasmid

   #99701

   Purpose

   Bacterial expression of RagA-RagC(Q120L)

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Use

   Tags

   His tag, Poly Arg, and SUMO

   Expression

   Bacterial

   Mutation

   Q120L

   Promoter

   T7

   Available since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Dendra2-NS2B

   Plasmid

   #204218

   Purpose

   Expresses Dendra2-NS2B in Mammalian / Mouse Cell Lines

   Depositor

   Partha Mondal

   Article

   Aravinth et al Sci Rep. 2025 Mar 26;15(1):10358. doi: 1

   Insert

   Dengue (Dengue Type 2) NS2B (POLY Dengue Virus Serotype , Strain NGC)
   

   Use

   Tags

   Dendra2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSCD-HCVP

   Plasmid

   #226227

   Purpose

   Express HCVP

   Depositor

   Michael Elowitz

   Article

   Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

   Insert

   HCVP (POLY Hepatitis C virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJEP224-AAV-Basic-0.4αCaMKII-GFP-pA

    Plasmid

    #62909

    Purpose

    AAV basic vector backbone designed to express GFP from a 0.4CaMKIIa promoter. It also contains a poly-Adenylation Signal (pA).

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    Green Fluorescent protein (GFP)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    0.4αCaMKII

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJEP221-AAV-RSV-GFP-pA

    Plasmid

    #62919

    Purpose

    AAV vector backbone designed to express GFP from a RSV promoter. It also contains a poly-Adenylation Signal (pA)

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    Green Fluorescent protein (GFP)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Rous Sarcoma Virus(RSV)

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT3TS-Hypa-nCas9n-A69

    Plasmid

    #125645

    Purpose

    micro-injection of poly-adenylated Hypa-nCas9n RNA

    Depositor

    Jason Berman

    Article

    Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

    Insert

    Hypa-nCas9n
    

    Use

    Rna in vitro transcription vector

    Tags

    Expression

    Mutation

    N692A, M694A, Q695A, H698A from wild-type Cas9

    Promoter

    T3

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pK RagA(R37P)-RagC

    Plasmid

    #99706

    Purpose

    Bacterial expression of RagA(R37P)-RagC

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag, Poly Arg, and SUMO

    Expression

    Bacterial

    Mutation

    R37P

    Promoter

    T7

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pK RagA-RagC(L91P)

    Plasmid

    #99707

    Purpose

    Bacterial expression of RagA-RagC(L91P)

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag, Poly Arg, and SUMO

    Expression

    Bacterial

    Mutation

    L91P

    Promoter

    T7

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pK RagA-RagC(K84T)

    Plasmid

    #99708

    Purpose

    Bacterial expression of RagA-RagC(K84T)

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag, Poly Arg, and SUMO

    Expression

    Bacterial

    Mutation

    K84T

    Promoter

    T7

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pK RagA(T21N)-RagC

    Plasmid

    #99650

    Purpose

    Bacterial expression of RagA(T21N)-RagC

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag, Poly Arg, and SUMO

    Expression

    Bacterial

    Mutation

    T21N

    Promoter

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pK RagA-RagC(S75N)

    Plasmid

    #99699

    Purpose

    Bacterial expression of RagA-RagC(S75N)

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag, Poly Arg, and SUMO

    Expression

    Bacterial

    Mutation

    S75N

    Promoter

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pK RagA(Q66L)-RagC(Q120L)

    Plasmid

    #99702

    Purpose

    Bacterial expression of RagA(Q66L)-RagC(Q120L)

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag, Poly Arg, and SUMO

    Expression

    Bacterial

    Mutation

    Q120L and Q66L

    Promoter

    T7

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHCMM1

    Plasmid

    #167452

    Purpose

    Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the TEF1 promoter

    Depositor

    Marvin Wickens, Hugo Cesar Medina-Munoz

    Article

    Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

    Insert

    PTEF1_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHCMM2

    Plasmid

    #167453

    Purpose

    Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the Sec63 promoter

    Depositor

    Marvin Wickens, Hugo Cesar Medina-Munoz

    Article

    Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

    Insert

    PSEC63_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PSEC63

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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