We narrowed to 1,196 results for: post

1. pAAV-HDR-mEGFP-Actin

   Plasmid

   #119870

   Purpose

   AAV vector including gRNA expression cassette and mEGFP knock-in donor targeting mouse Beta Actin

   Depositor

   Ryohei Yasuda

   Article

   Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

   Insert

   beta Actin (Actb Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMTS_mScarlet-i_N1

   Plasmid

   #85059

   Purpose

   In vivo visualization of the mitochondria (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   MTS (COX8A Human)
   

   Use

   Tags

   mScarlet-i

   Expression

   Mammalian

   Mutation

   aa1-29

   Promoter

   CMV

   Available since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. FHL-cpmTq2-Calcium-lifetime-sensor

   Plasmid

   #129628

   Purpose

   Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

   Depositor

   Dorus Gadella

   Article

   van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

   Insert

   FHL-cpmTq2-Calcium-lifetime-sensor
   

   Use

   Tags

   6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to …

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV,&Rhamnose

   Available since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. retro-puro vector

   Plasmid

   #58250

   Purpose

   Retroviral expression vector encoding an empty IRES-Puromycin cassette

   Depositor

   Georgios Stathopoulos

   Article

   Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMTS_mScarlet_N1

   Plasmid

   #85057

   Purpose

   In vivo visualization of the mitochondria (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   MTS (COX8A Human)
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   aa1-29

   Promoter

   CMV

   Available since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-HDR-mEGFP-camk2a

   Plasmid

   #104589

   Purpose

   AAV vector including gRNA expression cassette and mEGFP knock-in donor targeting mouse camk2a

   Depositor

   Ryohei Yasuda

   Article

   Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

   Insert

   calcium/calmodulin-dependent protein kinase II alpha (Camk2a Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. Myh6-Cas9

   Plasmid

   #109037

   Purpose

   Cardiac-specific overexpression of Cas9

   Depositor

   Eva van Rooij

   Article

   Johansen et al Circ Res. 2017 Oct 27;121(10):1168-1181.

   Insert

   Cas9
   

   Use

   Tags

   3XFlag

   Expression

   Mammalian

   Mutation

   Promoter

   alpha MHC

   Available since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Lck-cpmTq2-Calcium-lifetime-sensor

   Plasmid

   #129627

   Purpose

   Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

   Depositor

   Dorus Gadella

   Article

   van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

   Insert

   Lck-cpmTq2-Calcium-lifetime-sensor
   

   Use

   Tags

   Lck

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1a-FLEX(frt)-GCaMP6f-WPRE

   Plasmid

   #118273

   Purpose

   pAAV vector for flippase dependent GCaMP6f expression under the control of EF1a promoter

   Depositor

   Naoshige Uchida

   Article

   Menegas et al Nat Neurosci. 2018 Sep 3. pii: 10.1038/s

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   6xHis (N terminal on insert), T7 epitope, and Xpr…

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-EF1a-flex-TVA

    Plasmid

    #69618

    Purpose

    Express recepter TVA for envA with AAV

    Depositor

    Naoshige Uchida

    Article

    Menegas et al Elife. 2015 Aug 31;4. doi: 10.7554/eLife

    Insert

    Avian sarcoma and leukemia virus receptor 950 (CD320 Synthetic, quail)
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 3xnls-cpmTq2-Calcium-lifetime-sensor

    Plasmid

    #129626

    Purpose

    Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

    Depositor

    Dorus Gadella

    Article

    van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

    Insert

    3xnls-cpmTq2-Calcium-lifetime-sensor
    

    Use

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMTS_mScarlet-H_N1

    Plasmid

    #85058

    Purpose

    In vivo visualization of the mitochondria (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    MTS (COX8A Human)
    

    Use

    Tags

    mScarlet-H

    Expression

    Mammalian

    Mutation

    aa1-29

    Promoter

    CMV

    Available since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCR3-FLAG-Gephyrin GC

    Plasmid

    #68818

    Purpose

    Flag tagged mammalian expression of GC domain

    Depositor

    Shiva Tyagarajan

    Article

    Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

    Insert

    Gephyrin (Gphn Rat)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    NIL

    Promoter

    CMV

    Available since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCR3-FLAG-Gephyrin G

    Plasmid

    #68817

    Purpose

    Geph G domain expression in mamalian cells

    Depositor

    Shiva Tyagarajan

    Article

    Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

    Insert

    Gephyrin (Gphn Rat)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    NIL

    Promoter

    CMV

    Available since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA4/TO/GFPZNF598-9P-9A

    Plasmid

    #141192

    Purpose

    Expresses GFP-ZNF598 mutated in polyproline streches in mammalian cells, can be used to make inducible cell line

    Depositor

    Simon Bekker-Jensen

    Article

    Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

    Insert

    ZNF598 (ZNF598 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    all 3 proline repeats are mutated to Alanine (9xP…

    Promoter

    CMV

    Available since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pmirGLO-Calb2-3'UTR

    Plasmid

    #68363

    Purpose

    Luciferase miRNA Target Expression Vector

    Depositor

    Rolf Stahel, Emanuela Felley-Bosco

    Article

    Kresoja-Rakic et al Front Genet. 2017 May 29;8:70. doi: 10.3

    Insert

    Calb2 3'UTR (CALB2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    C to T change in UTR

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pmirGlo-CALB2-3'UTR mir-30 mut2

    Plasmid

    #74429

    Purpose

    Calb2 UTR with mutated mir-30 site 1 and 2

    Depositor

    Rolf Stahel, Emanuela Felley-Bosco

    Article

    Kresoja-Rakic et al Front Genet. 2017 May 29;8:70. doi: 10.3

    Insert

    CALB2 3'UTR (CALB2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOP795

    Plasmid

    #235700

    Purpose

    Protein expression His-taged HsNRBF2_FL in bacteria

    Depositor

    Roger Williams

    Article

    Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

    Insert

    NRBF2 (NRBF2 Human)
    

    Use

    Tags

    His6(TEV)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOP796

    Plasmid

    #235701

    Purpose

    Protein expression of His-tagged HsNRBF2 MIT domain in bacteria

    Depositor

    Roger Williams

    Article

    Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

    Insert

    NRBF2 MIT domain (NRBF2 Human)
    

    Use

    Tags

    His6(TEV)

    Expression

    Bacterial

    Mutation

    aa 1-89

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIA123

    Plasmid

    #234038

    Purpose

    E. coli-C. difficile shuttle vector for CRISPR editing in C. difficile. Pxyl::Cas9-opt Pgdh::sgRNA-cdr_0985. PstI site to add DNA for homology directed repair. MscI and NotI for replacement of sgRNA.

    Depositor

    Craig Ellermeier

    Article

    Bollinger et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pgdh and Pxyl

    Available since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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