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pLPC-MYC.Mm.RAP1.delta.MYB

Plasmid

#184660

Purpose

Retroviral expression of mouse RAP1 delta MYB

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

rap1

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

rap1 delta MYB

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC.Mm.RAP1.delta.COIL

Plasmid

#184659

Purpose

Retroviral expression of mouse RAP1 delta COIL

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

pLPC

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

rap1 delta COIL

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC.Mm.RAP1.delta.BRCT

Plasmid

#184658

Purpose

Retroviral expression of mouse Rap1 delta BRCT

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

RAP1

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

rap1 delta BRCT

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC.Mm.RAP1.delta.RCT

Plasmid

#184661

Purpose

Retroviral expression of mouse RAP1 delta RCT

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

rap1

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

rap1 delta RCT

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pPyCAG-NE-EGFP-IRES-Pac

Plasmid

#183617

Purpose

Mammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered EGFP (NLS-EGFP-EmerinTMD). Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Dev Cell. 2019 Jun 11. pii: S1534-5807(1

Insert

EGFP

Tags

Human EMD transmembrane domain and NLS

Expression

Mammalian

Promoter

CAG

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-mHP1alpha

Plasmid

#181911

Purpose

Bacterial protein expression vector for HP1alpha

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1alpha (Cbx5 Mouse)

Expression

Bacterial

Promoter

T7

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiGuide-PolB1-puro

Plasmid

#177146

Purpose

Lentiviral vector expressing PolB-gRNA-1 and a puromycin resistance cassette

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

PolBgRNA1 (POLB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B H85E

Plasmid

#175027

Purpose

Expresses WIPI2B (H85E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

Depositor

Andrea Stavoe

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2B (H85E) (WIPI2 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

Histadine 85 to Glutamate (CAC to GAG)

Promoter

CMV

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-mCherry-Bin1b

Plasmid

#176025

Purpose

Leishmania cell free expression of zebrafish mCherry-Bin1b. Parton lab clone GSU

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Bin1b (bin1b Zebrafish)

Use

Leishmania cell free expression

Tags

mCherry

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pZA68

Plasmid

#174781

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E/D481V-eGFP, Kmr plant selection marker

Tags

eGFP

Expression

Plant

Mutation

L17E/D481V

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1-YAPS127A-deltaCC

Plasmid

#166459

Purpose

Expresses fusion of mEGFP and YAPS127A-deltaCC (delta298-359)

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A-deltaCC (YAP1 Human)

Expression

Mammalian

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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Clim-DC1-mCherry-H2B-Venus

Plasmid

#129559

Purpose

nuclear marker

Depositor

Inaki Ruiz-Trillo

Insert

mcherry and Histone 2B fused to venus

Expression

Bacterial

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR1A_mmFbxw7(res) (closed)

Plasmid

#160105

Purpose

Expresses murine Fbxw7 alpha with a T199 silent mutation in mammalian cells. The T199 silent mutation confers resistance to the 5'-TGAACATGGTACAAGGCCAG-3' sgRNA

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Fbxw7alpha (Fbxw7 Mouse)

Use

Gateway Cloning

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pZA13

Plasmid

#158491

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 without STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1

Use

Golden gate entry vector

Mutation

no STOP codon

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-Mm_MyoGEF(WT)

Plasmid

#136325

Purpose

Mammalian expression of mouse MyoGEF-WT with N-terminal EGFP

Depositor

Guillermo de Cárcer, Marcos Malumbres

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

PLEKHG6 (Plekhg6 Mouse)

Expression

Mammalian

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pIW069

Plasmid

#134133

Purpose

Retroviral expression of GFP-Ska1

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

GFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

CRISPR resistant

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-2TKN-bp1

Plasmid

#133428

Purpose

Human BP1 fragment of SREBF1 in vector for bacterial expression of GST fusion protein.

Depositor

Gerhard Wagner

Article

Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

Insert

SREBF1 (SREBF1 Human)

Expression

Bacterial

Mutation

SREBP1a amino acids 1-50 fused to GST

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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SPDEF_pet28a

Plasmid

#131643

Purpose

Bacterial expression of ETS gene SPDEF

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

SPDEF (SPDEF Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AUG-stop-nLuc-3XFLAG

Plasmid

#127308

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon, but translation terminated immediately by stop codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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phSOD1-E100G-N’His

Plasmid

#117706

Purpose

mutation on stability/solubility of SOD1

Depositor

Elizabeth Fisher

Insert

SOD1 (SOD1 Human)

Tags

His tag with Thrombin cleavage site

Expression

Bacterial

Mutation

E100G

Promoter

T7

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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