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Plasmid#223429PurposeT-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.DepositorInsert2x35s-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro (PX459)
Plasmid#48139PurposeNOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.DepositorInserthSpCas9-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianPromoterCbhAvailable SinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT58
Plasmid#223430PurposeT-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.DepositorInsertZmUbi-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEGFP hsp70
Plasmid#15215DepositorAvailable SinceJune 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBR322-sgRNA-pvc18-20-Pdp1_NTD-Cre
Plasmid#243748PurposePVC cargo/regulatory region - deleted toxin cargos (Pdp1/Pnf) - vegfa sgRNA - Cre loaded into the PVC tube via Pdp1 (NTD)DepositorInsertvegfa sgRNA-pvc18-20-Pdp1_NTD-Cre
ExpressionBacterialAvailable SinceSept. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
10-4 cyclin E promoter
Plasmid#8458DepositorAvailable SinceJuly 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pYC-N
Plasmid#207113PurposeHDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locusDepositorTypeEmpty backboneUseCRISPRAvailable SinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJ151-HDR
Plasmid#113631PurposeContains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.DepositorInsertsLeft homology arm
Puromycin resistance gene
Venus fluorescence gene
Thymidine kinase
Right homology arm
UseCre/Lox; Homology recombination selection vectorExpressionBacterial and MammalianPromoterEF1a promoter, EF1a promoter (T2A cleaving sequen…Available SinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tol2-krt4-GFP
Plasmid#97151Purposelabel apical epithelial cells in zebrafishDepositorInsertAcGFP
UseZebrafishAvailable SinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-FnCas12a-TEV
Plasmid#174658PurposeFnCas12a expression plasmidDepositorInsertFnCas12a
UseCRISPRExpressionBacterialAvailable SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrex-n-eGFP
Plasmid#62544PurposeExpression of eGFP in T. cruzi. The NheI-linearized form of this plasmid could be efficiently integrated in to T. cruzi genomeDepositorInserteGFP
Use(1)trypanosoma cruzi expression of egfp (2) genom…TagsNoneMutationNonePromoterRibo-HX1Available SinceApril 16, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
CIRC-V1-Ana-CVB3 IRES-EGFP-BbsI
Plasmid#246754PurposeRNA circularization with CIRC using Ana intron, harbouring an CVB3 IRES-EGFP reporter.Plasmid was linearized with BbsI.DepositorInsertCIRC-V1-Ana-CVB3 IRES-EGFP-BbsI
UseSynthetic BiologyAvailable SinceOct. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
CIRC-V1-Ana-CVB3 IRES-EGFP-BsaI
Plasmid#246755PurposeRNA circularization with CIRC using Ana intron, harbouring an CVB3 IRES-EGFP reporter.Plasmid was linearized with BsaI.DepositorInsertCIRC-V1-Ana-CVB3 IRES-EGFP-BsaI
UseSynthetic BiologyAvailable SinceOct. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
CIRC-Scarless-Ana-CVB3 IRES split 403-mCherry
Plasmid#246753PurposeGenerate scarless circRNA with CVB3 IRESDepositorInsertCIRC-Scarless-Ana-CVB3 IRES split 403-mCherry
UseSynthetic BiologyAvailable SinceNov. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBES1-LacOx50-Hyg
Plasmid#210261PurposeIntegrates 50xLacO repeats upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.DepositorInsert50xLacO repeats derived from: PMID: 12864855.
Available SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-Ad5_HUHe-Pvc10-Cas9
Plasmid#243744PurposePVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike tip (Pvc10) loaded with HUHe on NTD and Cas9 on CTDDepositorInsertpvc13-Ad5_HUHe-Pvc10-Cas9
ExpressionBacterialAvailable SinceSept. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT56
Plasmid#223428PurposeT-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.DepositorInsertZmUbi-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBS-L1PA1-CH-mneo
Plasmid#51288Purposecontains the fully codon optimized ORF1 and ORF2 of the human L1RPDepositorInsertL1 (L1RE1 Human)
TagsmneoI indicator cassette containing an inverted n…ExpressionMammalianPromoterCMVAvailable SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
single cut NH-donor
Plasmid#83575PurposeeGFP reporter donor containing single SA cutting site for NHEJ knock-inDepositorInserteGFP
UseNhej donorAvailable SinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -