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hA3A-BE3 (pRZ215)

Plasmid

#131314

Purpose

CAG promoter expression plasmid for hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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Flrt2-Fc-His

Plasmid

#72074

Purpose

Expresses the extracellular region of the FLRT2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Flrt2 (Flrt2 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Anti-PICK1 [L20/8R]

Plasmid

#128630

Purpose

Mammalian Expression Plasmid of anti-PICK1 (Human). Derived from hybridoma L20/8.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-PICK1 (Human) recombinant mouse monoclonal antibody (PICK1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-mir144~451 EF1Alpha-puro-T2A-BFP

Plasmid

#164793

Purpose

Express miR-144~451 under the U6 promoter in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144~451 (MIR451A, MIR144 Human)
puro-T2A-BFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBa-FRB-3myc-KIF13B tail 442-1826

Plasmid

#64289

Purpose

KIF13A aa 442-1826 with N-terminal FRB and triple myc tags

Depositor

Gary Banker, Marvin Bentley

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

KIF13B (Kif13b Mouse)

Tags

3myc and FRB

Expression

Mammalian

Mutation

aa 442-1826

Promoter

chicken Beta Actin

Available Since

July 14, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP-Nsp8

Plasmid

#165114

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp8 (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Neuroligin-1 [N97A/31R]

Plasmid

#114554

Purpose

Mammalian Expression Plasmid of anti-Neuroligin-1 (Rat). Derived from hybridoma N97A/31.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Neuroligin-1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Nlgn1 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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Nsp10-EGFP

Plasmid

#165115

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp10 (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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Nsp13 K289A-EGFP

Plasmid

#165130

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp13 K289A (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-hGATE-16

Plasmid

#87870

Purpose

Express a EGFP-human GATE-16 in mammalian cells

Depositor

Eiki Kominami, Isei Tanida

Article

Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

Insert

GATE-16 (GABARAPL2 Human)

Expression

Mammalian

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLPC-Progerin-CS

Plasmid

#69064

Purpose

encodes Progerin with a substitution of cysteine in CaaX-motif by a serine

Depositor

Gerardo Ferbeyre

Article

Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d

Insert

Progerin (LMNA Human)

Use

Retroviral

Expression

Mammalian

Mutation

Progerin with substitution of cysteine in CaaX-mo…

Promoter

CMV

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only
LENTICRISPR-HK2_sgRNA1

Plasmid

#201596

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

HK2 (HK2 Human)

Use

CRISPR and Lentiviral

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLINK cyclin T1 (1-280) myc (K93D) (P#580)

Plasmid

#14611

Depositor

Matija Peterlin

Article

Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

Insert

cyclin T1 (1-280) K93D (CCNT1 Human)

Tags

myc

Expression

Mammalian

Mutation

K93D. Deficient in binding to CDK9. Inactive in 3…

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pTET-IUPi

Plasmid

#122019

Purpose

Expresses IUP pathway genes (ChK, IPK, idi) under inducible promoter. aTC inducible. Kan. resist.

Depositor

Gregory Stephanopoulos

Article

Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

Inserts

Choline kinase (CKI1 Budding Yeast)
Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
isopentenyl-diphosphate Delta-isomerase

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pTET

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)mGAT1YFP3

Plasmid

#41667

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)

Tags

YFP

Expression

Mammalian

Mutation

“Monomerizing” YFP A206K mutation; 3 C-terminal-m…

Promoter

CMV

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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Lrrc4c-Fc-His

Plasmid

#72085

Purpose

Expresses the extracellular region of the LRRC4C protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Lrrc4c (Lrrc4c Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-3xHA-SAPAP3

Plasmid

#190200

Purpose

Expresses SAPAP3 protein fused with an HA tag at the N-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

3xHA-SAPAP3 (Dlgap3 Mouse)

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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hAID-BE3 (pRZ352)

Plasmid

#131316

Purpose

CAG promoter expression plasmid for hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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Flrt1-Fc-His

Plasmid

#72073

Purpose

Expresses the extracellular region of the FLRT1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Flrt1 (Flrt1 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_UBA/UIM/ UBM-mut

Plasmid

#187154

Purpose

mammalian cell expression of FLAG-HUWE1 L2976A/P2977A/M1328A/F1330A/A1376G/S1382A

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

1xFLAG

Expression

Mammalian

Mutation

L2976A/P2977A/M1328A/F1330A/A1376G/S1382A

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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