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pDGE78

Plasmid

#80580

Purpose

recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:nptII-tmos
pPcUbi:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE79

Plasmid

#80581

Purpose

recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:PAT-tnos
pPcUbi:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pRCIscript-crhr2_TAL1A

Plasmid

#44236

Depositor

Karl Clark, Stephen Ekker

Article

Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

Insert

crhr2 pTAL scaffold 1A

Use

Mrna transcription vector

Promoter

T3

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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pRCIscript-crhr2_TAL1B

Plasmid

#44237

Depositor

Karl Clark, Stephen Ekker

Article

Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

Insert

crhr2 pTAL scaffold 1B

Use

Mrna transcription vector

Promoter

T3

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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DoS2

Plasmid

#244467

Purpose

Containing RP4 oriT, RP4 inc and I-SceI enzyme sequence, for eliminating RP4 plasmid and self-cutting.

Depositor

Lingchong You

Article

Tsoi et al Sci Adv. 2025 Apr 4;11(14):eads4735. doi

Insert

I-SceI enzyme (CSS3 Synthetic)

Use

Synthetic Biology

Promoter

tetR/tetA promoter

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pBA1729

Plasmid

#246063

Purpose

Bacterial expression of Panoptes locus with 300bp of context upstream and 150bp downstream of the ECOR31 operon cloned under the pTet promoter; p15a ori

Depositor

Jennifer Doudna

Article

Doherty et al bioRxiv [Preprint]. 2025 Apr 1:2025.03.2

Insert

Panoptes locus

Expression

Bacterial

Promoter

pTet promoter

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pMDC32B-SlmiR482bTS-B/c

Plasmid

#234369

Purpose

Cloning syn-tasiRNA sequences in minimal SlmiR482bTS precursors for expression in tomato

Depositor

Alberto Carbonell

Article

Tomassi et al Plant Biotechnol J. 2025 Oct 14. doi: 10

Inserts

B/c
SlmiR482bTS

Expression

Plant

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_2-3a_004_atpA_Promoter+5'UTR_Cr

Plasmid

#235876

Purpose

atpA promoter + 5'UTR Chlamydomonas reinhardtii 2-3a

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

atpA promoter + 5'UTR

Use

Synthetic Biology

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_3_009_psbF_5'UTR_Cr

Plasmid

#235837

Purpose

psbF 5'UTR Chlamydomonas reinhardtii

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

psbF 5'UTR

Use

Synthetic Biology

Mutation

none

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_6_026_3C6SF

Plasmid

#235954

Purpose

3' Connector 6 Short Forward in the M2 position used to dictate position for lvl2 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

3C6SF

Use

Synthetic Biology

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_2_064_Synthetic_promoter_08

Plasmid

#235817

Purpose

Synthetic promoter 08 based on Chlamydomonas rrn16 promoter

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

Synthetic promoter 08

Use

Synthetic Biology

Mutation

none

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_5_030_rpoB2_3'UTR_Cr

Plasmid

#235921

Purpose

rpoB2 3'UTR Chlamydomonas reinhardtii

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

rpoB2 3'UTR

Use

Synthetic Biology

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_6_005_psbH_3'Hom_Cr

Plasmid

#235939

Purpose

3' Homology psbH in the M2 position used for genome integration at the psbH locus

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

psbH 3'Homology

Use

Synthetic Biology

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_3_013_psaB_5'UTR_Cr

Plasmid

#235840

Purpose

psaB 5'UTR Chlamydomonas reinhardtii

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

psaB 5'UTR

Use

Synthetic Biology

Mutation

none

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_3IEE_023_IEE5

Plasmid

#235869

Purpose

tscA-chlN IEE5 Chlamydomonas reinhardtii

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

IEE5

Use

Synthetic Biology

Mutation

none

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_5_020_psaC_3'UTR_Cr

Plasmid

#235917

Purpose

psaC 3'UTR Chlamydomonas reinhardtii

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

psaC 3'UTR

Use

Synthetic Biology

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_3_015_psbC_5'UTR_Cr

Plasmid

#235842

Purpose

psbC 5'UTR Chlamydomonas reinhardtii

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

psbC 5'UTR

Use

Synthetic Biology

Mutation

none

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_3_001_5'UTR_placeholder

Plasmid

#235830

Purpose

Encodes a sfGFP cassette in the 5'UTR position used for placeholder cloning

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5'UTR placeholder

Use

Synthetic Biology

Mutation

none

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_3_032_rbcL_5'UTR_full_Nt

Plasmid

#235851

Purpose

rbcL 5'UTR full Nicotiana tabacum

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

rbcL full 5'UTR Nt

Use

Synthetic Biology

Mutation

none

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCK761

Plasmid

#242916

Purpose

Constitutive expression of dCas9, αNTD-MCP, and PspF-λN22

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

PspF-λN22 (pspF Synthetic)

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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