We narrowed to 10,080 results for: Gnas
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Plasmid#213173PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only
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MCHR1-DuET
Plasmid#213337PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-FIRE-pHLy
Plasmid#170774PurposeRatiometric biosensor expressed with UbC promoter for probing lysosomal pH in mammalian cellsDepositorInsertLAMP1 (LAMP1 Human)
UseLentiviralTagsmCherry and mTFP1ExpressionMammalianMutation84 nucleotide Signal Sequence switched to the beg…PromoterhUbCAvailable SinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
DRD2-DuET
Plasmid#213228PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA GNSTM-3-RVG-10-Lamp2b-HA
Plasmid#71294PurposeEncodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.DepositorInsertLamp2b (LAMP2 Human)
TagsGNSTM glycosylation motif, HA, Lamp2 signal pepti…ExpressionMammalianPromoterCMVAvailable SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
HTR7-DuET
Plasmid#213327PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUltraI-Tet3.0[TAA]
Plasmid#164580PurposeMachinery plasmid containing a single copy of the Mb Tet3.0RS and cognate tRNA for incorporation of Tet3.0 at TAA codonsDepositorInsertsMb Tet3.0RS (R2-3)
Mm tRNA[TAA]
ExpressionBacterialMutationL170G, N311G, C313APromoterProK and TacIAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_erGAP3
Plasmid#78118PurposeLow affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulumDepositorInserterGAP3
TagsKDEL and calreticulin signal peptideExpressionMammalianMutationS175G, D180Y, I167V and L15Q in GFP moiety and D…PromoterCMVAvailable SinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmBanana-Hyg-CTCF-CtermNES-N1
Plasmid#131851PurposeExpresses the [C-terminus of human CTCF (a.a. 577-728)]-mBanana with a nuclear export signal in mammalian cellsDepositorInsertCTCF (CTCF Human)
TagsmBananaExpressionMammalianMutationthe C-terminus of CTCF is linked to HIV-Rev prote…Available SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
MCHR2-DuET
Plasmid#213338PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR I-Ab beta Derp1-117
Plasmid#113639PurposeInsect cell expression vector for I-Ab beta chain fused to Der p 1 117-127 antigenic peptideDepositorInsertI-Ab beta Derp1-117 (H2-Ab1 Mouse)
Tags6x His tag, fused to Der p 1 (117-127) peptide vi…ExpressionInsectMutationtransmembrane and cytoplasmic domains truncatedPromoterMetallothioneinAvailable SinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
ADORA2B-DuET
Plasmid#213175PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FIRE-pHLy
Plasmid#170775PurposeRatiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cellsDepositorInsertLAMP1 (LAMP1 Human)
UseLentiviralTagsmCherry and mTFP1ExpressionMammalianMutation84 nucleotide Signal Sequence switched to the beg…PromoterCMVAvailable SinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
GLP1R-DuET
Plasmid#213253PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
HCTR2-DuET
Plasmid#213311PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-mito-R-GECO1
Plasmid#46021PurposeRed intensiometric genetically encoded Ca2+-indicators for optical imagingDepositorInsertR-GECO1
Tagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationSubstitutions relative to the mApple-derived anal…PromoterCMVAvailable SinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
Mito-PyronicSF/pCMV-myc-mito
Plasmid#124813PurposeHighly resposive GFP-based pyruvate nanosensor to explore mitochondrial metabolism.DepositorInsertPyronicSF
TagsMitochondrial Sequence SignalExpressionMammalianPromoterCMVAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
eMscL WT
Plasmid#107454PurposeeMscL WT is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motifDepositorInsertbacterial mechanosensitive ion channel of large conductance (mscL Escherichia Coli)
UseAdeno-associated viralTagsKir2.1 ER export signal (FCYENEV) and tdTomato fl…ExpressionMammalianPromoterhuman synapsin 1Available SinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRRL.SIN.EF1A.CD19-FMC63.218.CAR-3G
Plasmid#194458PurposeCAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.DepositorInsertAnti-CD19 CAR
UseLentiviralAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-mito-GEM-GECO1
Plasmid#32461PurposeMammalian expression of mitochondria-targeting blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imagingDepositorInsertGEM-GECO
Tagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationGCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…PromoterCMVAvailable SinceDec. 5, 2011AvailabilityAcademic Institutions and Nonprofits only