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pGV3340

Plasmid

#170278

Purpose

Expression of mutant HIV-2 RT p51 reverse transcriptase

Depositor

Gregory Van Duyne

Article

Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

Insert

HIV-2 RT p51 (mutant)

Tags

Hexahistidine

Expression

Bacterial

Promoter

T7-lac

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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HA-HIF2α P405A/P531A/N847A-pBabe-Puro

Plasmid

#25956

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 405 to Alanin…

Available Since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

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hum alpha ENAC promoter a25-a23

Plasmid

#83427

Purpose

promoter reporter construct to test 5' flanking sequences

Depositor

Christie Thomas

Article

Sayegh et al J Biol Chem. 1999 Apr 30;274(18):12431-7

Insert

human aENaC gene 5' flanking seq + 55 nt of the 5' UTR for exon 1A (SCNN1A Human)

Use

Luciferase

Tags

Luciferase

Promoter

alpha ENAC

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-3*HA-Palmd

Plasmid

#216833

Purpose

expressing HA tag and the coding sequence of mouse Palmd

Depositor

Yuxuan Guo

Article

Guo et al Acta Pharmacol Sin. 2024 Jul 23. doi: 10

Insert

Palmd (mus musculus) (Palmd Mouse)

Tags

HA

Expression

Mammalian

Available Since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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AAV_Actb NHEJ donor

Plasmid

#97316

Purpose

NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb NHEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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Chr8q_Centromere-Targeting_gRNA

Plasmid

#195128

Purpose

gRNA targeting centromere-proximal location on Chromosome 8q in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr8q gRNA

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6A NLRP1 UPA-CARD-FLAG WT

Plasmid

#163979

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 24, 2021

Availability

Academic Institutions and Nonprofits only

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mouse a2b full length

Plasmid

#217812

Purpose

Full length mouse integrin a2b expression

Depositor

Timothy Springer

Article

Hao et al MAbs. 2024 Jan-Dec;16(1):2365891. doi: 1

Insert

ITGA2B (Itga2b Mouse)

Tags

P2A-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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33-G-X5-pEF1/V5-His

Plasmid

#111605

Purpose

Express human 33-G-X5 in 293T cells

Depositor

Timothy Springer

Article

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

human LRRC33 and GARP chimeria (NRROS Human)

Tags

Flag tag

Expression

Mammalian

Promoter

EF1a

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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single polypeptide FPX biosensor for both caspase-3 and -8

Plasmid

#60886

Purpose

FPX biosensor

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

single polypeptide FPX biosensor for caspase-3 and 8

Tags

HindIII site and NES sequence LALKLAGLDIGS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only
pGV3319

Plasmid

#170277

Purpose

Expression of mutant Bst polymerase (Bst-LF D720A) for loop mediated isothermal amplification (LAMP)

Depositor

Gregory Van Duyne

Article

Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

Insert

Bst-LF D720A

Tags

Hexahistidine

Expression

Bacterial

Mutation

D720A

Promoter

T7-lac

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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AAV_Actb MMEJ donor

Plasmid

#97315

Purpose

MMEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb MMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pNL-CEF-IgG1-IRES-GFP

Plasmid

#187008

Purpose

Expression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgG1

Depositor

Hermann Eibel

Article

Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

Insert

Human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgG1

Use

Lentiviral

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA5-TEX264-deltaLRR-cSSH

Plasmid

#220210

Purpose

Mammalian expression of TEX264, 2-25 residues (LRR) truncated, C-terminal twin-Strep_HA tag

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Tags

Strep-strep-HA

Expression

Mammalian

Mutation

c.4-75del / p.2-24del

Promoter

CMV/TetO

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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MDH1-miR-31-PGK-GFP-Blast

Plasmid

#31515

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-31 (Mir31 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pLVX-TetOne-Puro-PFDN2

Plasmid

#202664

Purpose

Tet-ON 3rd generation expression vector with inducible expression of PFDN2 and a puromycin selection cassette

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

prefoldin subunit 2 (PFDN2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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Rosa26_gRNA

Plasmid

#196138

Purpose

gRNA targeting the Rosa26 locus in a thid generation Cas9 backbone with TagBFP2

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Rosa26 gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2 RB1 sgRNA #2

Plasmid

#202519

Purpose

Expressing Cas9 and sgRNA targeting human RB1 gene

Depositor

Ming Chen

Article

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

RB1 sgRNA (RB1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19_TELO2_dTAG

Plasmid

#224380

Purpose

Generates TELO2 dTAG N terminal knock-in cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

TELO2 (TELO2 Human)

Tags

dTAG

Expression

Bacterial

Mutation

Synonymous mutation

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19_GNB1L_dTAG

Plasmid

#224378

Purpose

Generates GNB1L dTAG C terminal knock-in cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Tags

dTAG

Expression

Bacterial

Mutation

Synonymous mutation

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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