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pGL3-p27Luc

Plasmid

#23047

Depositor

William Kaelin

Article

Zhang et al Nat Med. 2004 Jun . 10(6):643-8.

Insert

p27 (CDKN1B Human)

Tags

Luciferase

Expression

Mammalian

Mutation

wt cDNA

Available Since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

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dABE

Plasmid

#124447

Purpose

Expresses dead Cas9 (dCas9)-ABE7.10

Depositor

George Church

Article

Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

Insert

dABE7.10

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-hIFITM3

Plasmid

#58461

Purpose

Expresses human IFITM3 with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (IFITM3 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
EGFP-Rab13 in pCSDEST2

Plasmid

#140879

Purpose

Expression of zebrafish GFP-tagged Rab13 in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab13 (rab13 Zebrafish)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDGE1108

Plasmid

#202772

Purpose

CRISPR-KO in Arabidopsis thaliana

Depositor

Johannes Stuttmann

Article

Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

Inserts

FAST marker
pRPS5a:zCas9_ttriple
ccdB cassette (BsaI-excisable)

Use

CRISPR

Expression

Plant

Available Since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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Flag-FOXP4

Plasmid

#153148

Purpose

Expression of Flag tagged human FOXP4 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXP4 (FOXP4 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pXJ-His-PDCD1LG2

Plasmid

#232660

Purpose

transient expresses PD-L2 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

PDCD1LG2 (PDCD1LG2 Human)

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt4

Plasmid

#35910

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt4 (WNT4 Human)

Expression

Mammalian

Mutation

Silent Mutation C (492) --> T

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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LentiGuidPuro-hTP73_sgRNA-1

Plasmid

#88855

Purpose

CRISPR KO of Trp73

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp73

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pMpGWB236

Plasmid

#68627

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pME-APEX2-NS

Plasmid

#67669

Purpose

Gateway entry clone containing APEX2 with no stop codon

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2

Use

Gateway multisite middle entry clone

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pMB22

Plasmid

#122244

Purpose

Lentivirus delivery for stable expression of AsCas12a-VPR

Depositor

Darjus Tschaharganeh

Article

Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

Insert

mammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator

Use

Lentiviral

Tags

NLS and VPR activator

Promoter

EFs

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-VEGFR2-225

Plasmid

#21306

Depositor

Donald Ingber

Article

Mammoto et al Nature. 2009 Feb 26. 457(7233):1103-8.

Insert

VEGFR2 promoter (KDR Human)

Use

Luciferase

Available Since

May 29, 2009

Availability

Academic Institutions and Nonprofits only
pCruzHA SIRT1 G261A

Plasmid

#10963

Depositor

Toren Finkel

Article

Nemoto et al J Biol Chem. 2005 Apr 22. 280(16):16456-

Insert

SIRT1 (Sirt1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

G261A

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pGEX-5X1 Flag RbBP5

Plasmid

#15551

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

RbBP5 (RBBP5 Human)

Tags

Flag and GST

Expression

Bacterial

Available Since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
SeeSaw Reporter (SSR1.0)

Plasmid

#50392

Purpose

SSR1.0 has a one I-SceI target site for generate specific double strand break after I-SceI endonuclease expression.

Depositor

Pablo Huertas Sánchez

Article

Gomez-Cabello et al PLoS One. 2013 Oct 14;8(10):e77206. doi:

Insert

SSR1.0

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pPLtetO-FtsZ

Plasmid

#89656

Purpose

Plasmid for titrating synthesis of FtsZ with doxycycline

Depositor

Uwe Sauer

Article

Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

Insert

GTP-binding tubulin-like cell division protein

Use

Synthetic Biology

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-rab7a

Plasmid

#109723

Purpose

Multisite gateway vector for 3' tagging with rab7a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab7a (rab7a Zebrafish)

Use

Zebrafish plasmids

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMVV207

Plasmid

#215343

Purpose

gRNA expression for S. stutzeri nifL deletion on an RSF1010 backbone

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

S. stutzeri nifL gRNA

Use

CRISPR and Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

J23119

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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p182 pK7-HDAC1 mut (GFP)

Plasmid

#11055

Depositor

Ramesh Shivdasani

Article

Tou et al Mol Cell Biol. 2004 Apr . 24(8):3132-9.

Insert

HDAC1 (Hdac1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Deletion of aa 1-56: deacetylase domain deletion

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only

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