Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 18,743 results for: emb

Clear keywords
Showing: 9321 - 9340 of 18743 results

  1. pGBW-m4046776

    Plasmid
    #145734
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145734-plasmid-map-sequence-id-298344

    Enlarge

  2. pGBW-m4046926

    Plasmid
    #145663
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145663-plasmid-map-sequence-id-352066

    Enlarge

  3. pGBW-m4046908

    Plasmid
    #145603
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;Myc
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145603-plasmid-map-sequence-id-298366

    Enlarge

  4. pGBW-m4046904

    Plasmid
    #145591
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145591-plasmid-map-sequence-id-298327

    Enlarge

  5. pETM11-SUMO3-KSR1-CA3-sfGFP (C-KSR)

    Plasmid
    #217766
    Purpose
    Fluorescent reporter for ceramide (for bacterial expression and purification)
    Depositor
    Paula Nunes-Hasler
    Publication
    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do
    Insert
    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    Use
    Protein purification (6xhis tag, tev site, sumo3 …
    Tags
    sfGFP
    Expression
    Bacterial
    Mutation
    CA3 domain aa 317-400 plus vector-based linker LE…
    Promoter
    T7 promoter
    Available since
    June 10, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    217766-plasmid-map-sequence-id-435787

    Enlarge

  6. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-PSMB5-PSMB6-PSMB7-TEV-2xSTII

    Plasmid
    #214139
    Purpose
    Insect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta2
    Depositor
    Brenda Schulman
    Publication
    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1
    Inserts
    PSMB1 (PSMB1 Human)
    PSMB2 (PSMB2 Human)
    PSMB3 (PSMB3 Human)
    PSMB4 (PSMB4 Human)
    PSMB5 (PSMB5 Human)
    PSMB6 (PSMB6 Human)
    PSMB7 (PSMB7 Human)
    Tags
    2xSTII
    Expression
    Insect
    Promoter
    polyhedrin
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    214139-plasmid-map-sequence-id-431260

    Enlarge

  7. pGEX-6p-1_GST-DmKHC[1-421]_1xmNeonGreen

    Plasmid
    #196974
    Purpose
    Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal mNeonGreen tag and cleavable N-terminal GST tag
    Depositor
    Lukas Kapitein
    Publication
    Chen et al Elife. 2022 Jul 5;11:e77892. doi: 10.755
    Insert
    Kinesin Heavy Chain (Khc Fly)
    Tags
    GST and mNeonGreen
    Expression
    Bacterial
    Promoter
    tac
    Available since
    July 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    196974-plasmid-map-sequence-id-388923

    Enlarge

  10. pEGFP-C1-GFP-SF3B6-Delete-NLS-FLAG

    Plasmid
    #86871
    Purpose
    To express SF3B6 delete NLS signal with a GFP and FLAG tags at N- and C-terminus, respectively.
    Depositor
    Yihong Ye
    Publication
    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SF3B6 (SF3B6 Human)
    Tags
    EGFP and FLAG
    Expression
    Mammalian
    Mutation
    amino acids 7-29 deletion
    Promoter
    CMV
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    86871-plasmid-map-sequence-id-175234

    Enlarge

  11. pJ137

    Plasmid
    #29487
    Depositor
    Jonathan Tait
    Publication
    Jin et al J Biol Chem 2004; 279:40351-57.
    Insert
    annexin V-128 (ANXA5 Human)
    Tags
    (Met)-Ala-Gly-Gly-Cys-Gly-His
    Expression
    Bacterial
    Mutation
    Deletion of the initiator Met at position 1 of wi…
    Available since
    May 17, 2011
    Availability
    Academic Institutions and Nonprofits only

  15. pAL725

    Plasmid
    #119862
    Purpose
    precusor D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C
    Depositor
    Linda Randall
    Publication
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C (mglB Escherichia coli str. K-12)
    Tags
    none
    Expression
    Bacterial
    Mutation
    Leucine 244 to Cysteine, Aspartate 287 to Cysteine
    Promoter
    T5
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    119862-plasmid-map-sequence-id-232032

    Enlarge

  16. DEP-GFP

    Plasmid
    #109043
    Purpose
    Expression of the Dishevelled DEP domain
    Depositor
    Mariann Bienz
    Publication
    Gammons et al Mol Cell. 2016 Oct 6;64(1):92-104. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dishevelled segment polarity protein 2 (DVL2 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Dvl2 DEP domain
    Promoter
    CMV
    Available since
    Aug. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    109043-plasmid-map-sequence-id-220610

    Enlarge

  17. ABCB8 (ABCB8A-c016)

    Plasmid
    #110266
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ABCB8 (ABCB8 Human)
    Tags
    TEV-His10-Flag
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    110266-plasmid-map-sequence-id-212276

    Enlarge

  20. pX330-sgRNA_Dicer_2

    Plasmid
    #68808
    Purpose
    specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of
    Depositor
    Constance Ciaudo
    Publication
    Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA mouse Dicer1
    Use
    CRISPR and Mouse Targeting
    Expression
    Bacterial and Mammalian
    Available since
    Feb. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    68808-plasmid-map-sequence-id-120930

    Enlarge

Showing: 9321 - 9340 of 18743 results