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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 19,605 results for: Met;

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Showing: 921 - 940 of 19605 results

  6. pPPC002

    Plasmid
    #171139
    Purpose
    For integration of J1-J23117-sfGFP, Sp.pCas9-dCas9, and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(
    Inserts
    J1-BBa_J23117-sfGFP
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    J1-BBa_J23117 and Sp.pCas9, BBa_J23107
    Available Since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    171139-plasmid-map-sequence-id-344756

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  8. STARR-seq luciferase validation vector_ORI_CMV

    Plasmid
    #99312
    Purpose
    Luciferase validation vector with CMV enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
    Depositor
    Alexander Stark
    Article
    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453
    Insert
    CMV enhancer
    Use
    Luciferase; Starr-seq luciferase validation vector
    Expression
    Mammalian
    Available Since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    99312-plasmid-map-sequence-id-190933

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  11. STARR-seq luciferase validation vector_ORI_IGF1R

    Plasmid
    #99306
    Purpose
    Luciferase validation vector with IGF1R enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
    Depositor
    Alexander Stark
    Article
    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453
    Insert
    enhancer; chr15: 99439352 -99440791
    Use
    Luciferase; Starr-seq luciferase validation vector
    Expression
    Mammalian
    Available Since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    99306-plasmid-map-sequence-id-190940

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  13. STARR-seq luciferase validation vector_SCP1_CMV

    Plasmid
    #99314
    Purpose
    Luciferase validation vector with CMV enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
    Depositor
    Alexander Stark
    Article
    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453
    Insert
    CMV enhancer
    Use
    Luciferase; Starr-seq luciferase validation vector
    Expression
    Mammalian
    Available Since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    99314-plasmid-map-sequence-id-190943

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  14. pAS_4xMma PylT_FLAG-PcKRS

    Plasmid
    #140022
    Purpose
    Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of photocaged-lysine; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006
    Insert
    PcKRS (pylS Methanosarcina bakeri)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    M241F, A267S, Y271C, L274M
    Promoter
    EF1
    Available Since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    140022-plasmid-map-sequence-id-297729

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  15. STARR-seq luciferase INF enhancer vector_ORI_OAS3

    Plasmid
    #99324
    Purpose
    Luciferase validation vector with OAS3 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
    Depositor
    Alexander Stark
    Article
    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453
    Insert
    enhancer; chr12: 94575894 -94578286
    Use
    Luciferase; Starr-seq luciferase validation vector
    Expression
    Mammalian
    Available Since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    99324-plasmid-map-sequence-id-193170

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  18. pAS_4xMma PylT_FLAG-AcKRS

    Plasmid
    #140021
    Purpose
    Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of acetyl-lysine; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006
    Insert
    AcKRS-3 (pylS Methanosarcina mazei)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    L301M, L305I, Y306F, L309A, C348F
    Promoter
    EF1
    Available Since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    140021-plasmid-map-sequence-id-297663

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  20. GANES-DEVD-BNLS

    Plasmid
    #50842
    Purpose
    Expresses a tandem green ddFP heterodimer in mammalian cells, construct 1 for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid RANLS.
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddGFP A and ddGFP B
    Tags
    NES sequence LALKLAGLDIGS placed after ddGFP A an…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    50842-plasmid-map-sequence-id-76290

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Showing: 921 - 940 of 19605 results