We narrowed to 4,109 results for: 272

1. pX330-TJP1

   Plasmid

   #227299

   Purpose

   Expresses SpCas9 and a sgRNA targeting the N-terminus of TJP1 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of TJP1 (TJP1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMM114

   Plasmid

   #127211

   Purpose

   BeYDV replicon with constitutive Luc expression, WUS2 and IPT, sgRNA targeting PDS

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   WUS2, IPT, sgRNA, Luc
   

   Use

   Tags

   Expression

   Plant

   Mutation

   codon optimized CDSs

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p663-UBC-TurboID-V5-BRSK2_IDG-K

   Plasmid

   #135272

   Purpose

   Gateway destination clone of BRSK2 (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   TurboID-V5-BRSK2 (BRSK2 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-TurboID

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. qTAG-C-mStayGold-EFS-Zeo

   Plasmid

   #227258

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Zeo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMM134

   Plasmid

   #127215

   Purpose

   BeYDV replicon with constitutive Luc expression, IPT, sgRNA targeting PDS

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   Luc, IPT, sgRNA
   

   Use

   Tags

   Expression

   Plant

   Mutation

   codon optimized CDSs

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMM113

   Plasmid

   #127210

   Purpose

   BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM, sgRNA targeting PDS

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   WUS2, AtSTM, sgRNA, Luc
   

   Use

   Tags

   Expression

   Plant

   Mutation

   codon optimized CDSs

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMM119

   Plasmid

   #127213

   Purpose

   BeYDV replicon with constitutive GFP expression, WUS2, sgRNA targeting PDS

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   GFP, WUS2, sgRNA
   

   Use

   Tags

   Expression

   Plant

   Mutation

   codon optimized CDSs

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TagBFP2-PIP4K2AD273K

   Plasmid

   #202727

   Purpose

   expresses fluorescent protein-tagged enzyme

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   mTagBFP2:PIP4K2A(D266K) (PIP4K2A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMM136

   Plasmid

   #127217

   Purpose

   BeYDV replicon with constitutive Luc expression, MP-delta, sgRNA targeting PDS

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   Luc, MP-delta, sgRNA
   

   Use

   Tags

   Expression

   Plant

   Mutation

   codon optimized CDSs

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMM146

    Plasmid

    #127218

    Purpose

    BeYDV replicon with constitutive Luc expression, BBM, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc, BBM, sgRNA
    

    Use

    Tags

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMM135

    Plasmid

    #127216

    Purpose

    BeYDV replicon with constitutive Luc expression, WUS2, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc, WUS2, sgRNA
    

    Use

    Tags

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMM131

    Plasmid

    #127214

    Purpose

    BeYDV replicon with constitutive Luc expression, AtSTM, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc, AtSTM, sgRNA
    

    Use

    Tags

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. qTAG-KO-PGK-Puro

    Plasmid

    #227264

    Purpose

    Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a PGK-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-Vinculin_TurboID_Venus

    Plasmid

    #200272

    Purpose

    Expressing vinculin with co-localization labeler TurboID and fluorescence reporter Venus

    Depositor

    Brenton Hoffman

    Article

    Tao et al Dev Cell. 2023 Mar 10:S1534-5807(23)0007

    Insert

    Vinculin_TurboID_Venus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYZ_3WJrep_N20_switch

    Plasmid

    #132722

    Purpose

    T7 RNAP-driven expression of 3WJ repressor index 20 switch RNA with a GFP-ASV reporter

    Depositor

    Peng Yin, Alexander Green

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    3WJ_N20_switch
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYZ_3WJrep_N20_trigger

    Plasmid

    #132726

    Purpose

    T7 RNAP-driven expression of 3WJ repressor index 20 trigger RNA

    Depositor

    Peng Yin, Alexander Green

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    3WJ_N20_trigger
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYZ_3WJrep_N24_switch

    Plasmid

    #132725

    Purpose

    T7 RNAP-driven expression of 3WJ repressor index 24 switch RNA with a GFP-ASV reporter

    Depositor

    Peng Yin, Alexander Green

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    3WJ_N24_switch
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAN2981

    Plasmid

    #127246

    Purpose

    Asymmetric degronSwitchA_t8 fused to mCherry with KeyA-BFP

    Depositor

    Hana El-Samad

    Article

    Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

    Insert

    pPGK-Key_A_e18-TagBFP-P2A-mCherry-asym_degronSwitch_A_t8-WPRE
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNP555

    Plasmid

    #222722

    Purpose

    sfGFP-ON BsaI Golden Gate Landing Pad Backbone for RE-LE (bRNA in cis expression)

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Type

    Empty backbone

    Use

    Tags

    sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYZ_3WJrep_N19_switch

    Plasmid

    #132723

    Purpose

    T7 RNAP-driven expression of 3WJ repressor index 19 switch RNA with a GFP-ASV reporter

    Depositor

    Peng Yin, Alexander Green

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    3WJ_N19_switch
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge