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pFSW GAP43-jRGECO1a P2A Syp-Halo
Plasmid#185565PurposeExpression of axonally-targeted jRGECO1a, a red Ca2+ sensor, along with synaptophysin-HaloTag in neuronal cells, separated by a P2A sequenceDepositorInsertGAP43-jRGECO1a P2A Syp-Halo
UseLentiviralTagsHaloTagExpressionMutationPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-USP5
Plasmid#61742Purposeexpress N-terminally FLAG tagged human USP5DepositorInsertUSP5 (USP5 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-GFP-Sec61β
Plasmid#186597PurposeThird generation lentiviral vector expressing GFP-Sec61βDepositorInsertSec61β (SEC61B Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Myc-GRM1-IRES-CyRFP1
Plasmid#195355PurposemGluR1-IRES-cyRFP1 under the control of the pCAG promoterDepositorInsertGRM1-IRES-CyRFP1
UseTagsExpressionMammalianMutationPromoterpCAGAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCGN-S2P-WT
Plasmid#32957PurposeFor mammalian expression of human S2P with an HA tagDepositorInsertS2P (MBTPS2 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLENTI-CMV-GFP-TREX1 D18N BLAST
Plasmid#164225Purposelentiviral plasmid for GFP-hTREX1 D18N (catalytically-deficient)DepositorInsertTREX1 (TREX1 Human)
UseLentiviralTagsGFPExpressionMammalianMutationD18NPromoterCMVAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRN3P_AID-Dam-only
Plasmid#136065Purposein vitro transcription for mRNADepositorInsertAID-Dam only
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cenp-B DBD INCENP GFP
Plasmid#45237PurposeTarget INCENP to centromeres via CENP-B DNA binding domain (DBD)DepositorInsertCenp-B-DBD, INCENP-Δcen, GFP
UseTagsDNA binding domain of CENP-B and GFPExpressionMammalianMutationCEN box deleted from INCENP (aa1-46)PromoterAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pStr-KDEL-IRES-ST-mCherry
Plasmid#110727PurposeExpresses an Str-KDEL Hook for the RUSH system and separately Sialyltransferase-mCherryDepositorInsertsUseTagsStr Streptavidin binding peptide (SBP) and mCherr…ExpressionMammalianMutationcontains only amino acids 1 to 43 of STPromoterCMV and IRESAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
EpsilonCOP EGFP
Plasmid#128151PurposeExpression of epsilon COPDepositorInsertepsilon COP (COPE Bovine)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLz37_CB_operon_CsoS2_N1-N4
Plasmid#140856PurposeHalothiobacillus neapolitanus carboxysome operon with full length CsoS2. For heterologous expression of carboxysomes in E. coli.DepositorInsertCbbL, CbbS, CsoS2 (full-length), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMTS-hFluc
Plasmid#177716PurposeFirefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targetingDepositorInserthFluc
UseTagsMitochondrial Targeting Sequence MTS SSExpressionBacterial, Mammalian, and Y…MutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRDV5 containing Sb-convex
Plasmid#132696PurposepRDV5 plasmid containing a convex Sybody for the generation of a qPCR standard curveDepositorInsertSb_convex including 5' and 3' flanking sequences used for ribosome display
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-EF20
Plasmid#52645Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-EF20
UseTagsExpressionMammalianMutationD362N/D366N mutated from GCaMP3PromoterT7Available sinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS_FLPER(T2)
Plasmid#31303DepositorInsertFLP-ER ligand binding domain (ESR1 Budding Yeast, Human)
UseTagsExpressionMammalianMutationfull length FLP linked to ligand binding domain o…PromoterAvailable sinceOct. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMRX-INU-TEX264 LIR4A-FLAG
Plasmid#128259PurposeExpresses TEX264 LIR4A in mammalian cellsDepositorInsertTEX264 LIR4A (TEX264 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationChanged FEEL (aa 273-276) to alaninesPromoterCMVAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET14-GFP30_Encap
Plasmid#86406PurposeExpresses sfGFP tagged with a 30 amino acid CLP and encapsulin as an operonDepositorInsertssfGFP with a 30aa CLP tag
Encapsulin
UseTags6x HistidineExpressionBacterialMutationPromoterT7Available sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
mOtop2_pcDNA3
Plasmid#114678PurposeThis construct was used to express mouse Otop2 in HEK-293 cellsDepositorInsertOtopetrin 2 (Otop2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET14-GFP15_Encap
Plasmid#86407PurposeExpresses sfGFP tagged with a 15 amino acid CLP and encapsulin as an operonDepositorInsertssfGFP with a 15aa CLP tag
Encapsulin
UseTags6x HistidineExpressionBacterialMutationPromoterT7Available sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only
