We narrowed to 20,158 results for: Nov
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Plasmid#106183PurposeTight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas serviceAAV1InsertSF-Venus-iGluSnFR.A184S
UseAAVMutationSFGFP: T203Y, F46L, T65G, S72A GltI: A184SPromoterhSynapsinAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAG-Flex-TC66T
Plasmid#48331PurposeCre-dependant TVA receptor-mCherry fusion with a mutation (66T) in TVADepositorInsertTVA receptor mCherry fusion with the 66T mutation
UseAAV and Cre/LoxTagsmCherry fusion to TVA receptorExpressionMammalianMutation66TPromoterCAGAvailable sinceDec. 6, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA-HA-ER Y537S
Plasmid#49499PurposeExpresses HA-tagged ER Y537S mutant in mammalian cellsDepositorAvailable sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LifeAct-mGreenLantern
Plasmid#164459PurposeLocalization of mGreenLantern fluorescent protein to actinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmGreenLantern
TagsLifeActExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTS117 His14-Avi-45xGS-anti GFP nanobody
Plasmid#199370PurposeBacterial expression plasmid for anti-GFP nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)DepositorInsertanti-GFP nanobody
Tags14xHis-AviExpressionBacterialMutationWTPromoterT5-LacOAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EC1000
Bacterial strain#71852PurposeRepA+ MC1000, KmR , carrying a single copy of the pWV01 repA gene in the glgB gene; host for pOR128-based plasmidsDepositorBacterial resistanceKanamycinAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAd-M3cherry
Plasmid#61041PurposeThis plasmid mediates polycistronic expression of 4 genes: Ngn3, Pdx1, Mafa, and mCherry. It can be used to convert mouse pancreatic acinar cells to induced beta-like cells in vivo.DepositorAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-TBG-ER-TurboID
Plasmid#149415PurposeHepatocyte-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selectionDepositorHas serviceAAV8InsertER-TurboID
UseAAVTagsHA and V5PromoterTBGAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-mem-TurboID
Plasmid#149409PurposeMammalian expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in mammalian expression plasmid. Amp/Neo selectionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmem-TurboID
TagsHA and V5ExpressionMammalianPromoterCMVAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA-HA-ER D538G
Plasmid#49500PurposeExpresses HA-tagged ER D538G mutant in mammalian cellsDepositorInsertESR1 (ESR1 Human)
TagsHAExpressionMammalianMutationAspartic acid 538 to GlycinePromoterCMVAvailable sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLCKO
Plasmid#73311PurposeLentiviral backbone for cloning and expressing U6 driven sgRNAs with BfuAI cloning sites and puromycin selection.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
C321.deltaA (Isaacs lab)
Bacterial strain#73581PurposeGenomically recoded organism (E. coli C321.A, CP006698.1, GI:54981157). This strain is different than Addgene #68306 as it still retains the serB geneDepositorBacterial resistanceAmpicillinAvailable sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MPC027: pAAV-CAG-FLEX-sNovArch-PP-Citrine_KV2.1TS-ER2 (soma targeting)
Plasmid#153196Purposeencodes pAAV soma targeted NovArchDepositorInsertsNovArch
UseAAVTagscitrineExpressionMammalianAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag::UbvG08 I44A, deltaGG
Plasmid#74939PurposeUbiquitin variant that binds to and occludes the ligand binding site of the 53BP1 Tudor domain (i53). Blocks 53BP1 from accumulating at sites of DNA damage. Potent selective inhibitor of 53BP1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUbiquitin
TagsFlagExpressionMammalianMutationUbvG08 with I44A mutation and no terminal GlycinesPromoterCMVAvailable sinceJune 30, 2016AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
p3xFLAG SARS-CoV2 RdRp.co
Plasmid#154008PurposeExpression of codon-optimized SARS-CoV2 Nsp12 (RdRp) in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCodon-optimized SARS-CoV2 Nsp12 from ORF1ab (ORF1ab Synthetic)
Tags3xFLAG tagExpressionMammalianPromoterCMVAvailable sinceJune 23, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEvol-pAcFRS.2.t1
Plasmid#73544PurposetRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAGDepositorInsertspAcFRS.2.t1
pAcFRS.2.t1
ExpressionBacterialAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSynapsin- soCoChR-GFP
Plasmid#107708PurposeA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Human Synapsin (hSynapsin) promoterDepositorInsertsoCoChR-GFP
UseAAVTagsEGFP and KA2ExpressionMammalianPromoterhSynapsinAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-i53
Plasmid#92170Purposerecombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant (i53) that is a selective inhibitor of 53BP1DepositorInserti53
UseAAVTagsFLAGExpressionMammalianMutationUbvG08 with I44A mutation and no terminal GlycinesPromoterCMVAvailable sinceJuly 13, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTubb3-MC
Plasmid#87112Purposeminicircle parental backbone plasmid including GFP knock-in donor targeting Tubb3 gene and one cutting site for HITIDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGFP
UseCRISPRAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
6-His-MBP-TEV-FnCpf1
Plasmid#90094PurposeBacterial expression plasmid for protein purificationDepositorInsertFnCpf1 (humanized)
Tags3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…ExpressionBacterialPromoterT7Available sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by