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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,938 results for: emb

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Showing: 921 - 940 of 11938 results

  4. pRluc-LC3G120A

    Plasmid
    #105003
    Purpose
    Mammalian expression of Rluc-LC3G120A. Can be used for measuring autophagic flux.
    Depositor
    Marja Jããttelã
    Publication
    Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LC3B (Map1lc3b Rat)
    Use
    Luciferase
    Tags
    Rluc with C124A substitution
    Expression
    Mammalian
    Mutation
    Glycine 120 mutated to alanine, prevents lipidati…
    Available since
    Jan. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105003-plasmid-map-sequence-id-202164

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  8. pMSCVpuro-Mito-Pericam

    Plasmid
    #87381
    Purpose
    Retroviral transfection of cells to express ratiometric Pericam as described by Nagai et al 2001 (PMID 11248055) localising to the mitochondrial matrix via the transit peptide from human COX8A.
    Depositor
    Björn Stork
    Publication
    Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do
    Insert
    Mito-Pericam [ratiometric]
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    Retroviral 5' LTR promoter
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87381-plasmid-map-sequence-id-212242

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  9. pD-MSP1D1

    Plasmid
    #161607
    Purpose
    Bacterial expression of mutated membrane scaffold protein ("dark" MSP1D1) for Nanodisc formation
    Depositor
    Stephen Sligar
    Publication
    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do
    Insert
    D-MSP1D1
    Tags
    7-His tag
    Expression
    Bacterial
    Mutation
    mutant of MSP1D1; all tryptophans were replaced b…
    Promoter
    T7
    Available since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    161607-plasmid-map-sequence-id-317653

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  10. pcDNA-LBR-EGFP-polyA

    Plasmid
    #59748
    Purpose
    Express LBR-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.
    Depositor
    Yi Zhang
    Publication
    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LBR-EGFP
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available since
    Sept. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    59748-plasmid-map-sequence-id-94052

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  14. pcDNA3.1 CARMA1

    Plasmid
    #44431
    Depositor
    Xin Lin
    Insert
    CARMA1 (CARD11 Human)
    Expression
    Mammalian
    Mutation
    See depositor comments below.
    Available since
    Oct. 9, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    44431-plasmid-map-sequence-id-263620

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  16. EHD2-mEGFP

    Plasmid
    #45932
    Purpose
    caveolar protein, confines caveolae to the plasma membrane
    Depositor
    Ari Helenius
    Publication
    Stoeber et al EMBO J. 2012 May 16;31(10):2350-64. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Eps-15 homology domain-containing protein2 (EHD2 Human)
    Tags
    monomeric EGFP_FrameN1
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    45932-plasmid-map-sequence-id-66945

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  19. RA-Sec61β

    Plasmid
    #153978
    Purpose
    Dimerization dependent fluorescent protein RA domain fused to ER protein Sec61β
    Depositor
    Gia Voeltz
    Publication
    Lee et al Science. 2020 Jan 31;367(6477). pii: 367
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sec61 Transolocon Subunit Beta (SEC61B Human)
    Tags
    pAcGFP-C1
    Expression
    Mammalian
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    153978-plasmid-map-sequence-id-306136

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  20. pSEP-LAMP1-RFP

    Plasmid
    #167970
    Purpose
    Expresses the chimeric construct SEP-LAMP1-RFP where superecliptic pHluorin (SEP) is fused to the luminal domain of LAMP1-RFP
    Depositor
    Juan Bonifacino
    Publication
    Farias et al Proc Natl Acad Sci U S A. 2017 Apr 4;114
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LAMP1 (Lamp1 Rat)
    Tags
    SEP and mRFP
    Expression
    Mammalian
    Mutation
    D50E
    Promoter
    CMV
    Available since
    April 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    167970-plasmid-map-sequence-id-331200

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Showing: 921 - 940 of 11938 results