We narrowed to 2,385 results for: fas
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Plasmid#34957DepositorPublicationInsertPSmOrange2
ExpressionBacterialMutationR17H, R36H, F64I, Q194L, A224S, G226A (mutations …Available sinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLSB-KAN
Plasmid#166700PurposeCombined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.DepositorInsertstRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe
ExpressionYeastPromoteradh15 promoter and tRNA Ser promoterAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EF1a-DIO-ASAP3Kv-WPRE
Plasmid#132330Purposeexpresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vectorDepositorInsertASAP3KV
UseAAV and Cre/LoxExpressionMammalianPromoterEF1aAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pH2B-PSmOrange2
Plasmid#34960DepositorPublicationInsertPSmOrange2
TagsH2BExpressionMammalianMutationR17H, R36H, F64I, Q194L, A224S, G226A (mutations …Available sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBS U6a gRNA
Plasmid#158961PurposegRNA backbone driven by zebrafish U6a promoterDepositorInsertszf U6a promoter
gRNA backbone
UseCRISPRAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU43
Plasmid#167665PurposeENTRY vector for gRNA3 cloning under pAtU6 promoterDepositorInsertpAtU6
UseCRISPRAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-myrSNAP
Plasmid#60098PurposeExpresses membrane-targeted SNAP(f)-tag and histone-GFP, non-conditionalDepositorInsertsSNAP-tag
histone-GFP
UseAAV and Mouse TargetingExpressionMammalianAvailable sinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EF1a-DIO-ASAP3-WPRE
Plasmid#132318Purposeexpresses ASAP3(ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vectorDepositorInsertASAP3
UseAAV and Cre/LoxExpressionMammalianPromoterEF1aAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCSN068
Plasmid#101749PurposeSingle copy yeast plasmid expressing Cpf1 from Francisella novicida U112 (FnCpf1), codon optimized for expression in Saccharomyces cerevisiae.DepositorInsertsCpf1 from Francisella novicida U112 (FnCpf1) codon optimized for expression in S. cerevisiae.
KanMX marker expression cassette.
UseCRISPRTagsSV40 NLSExpressionYeastPromoterHeterologous TEF1 promoter from A. gossypii. and …Available sinceJan. 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLSB-HYG
Plasmid#166699PurposeCombined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.DepositorInsertstRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe
ExpressionYeastPromoteradh15 promoter and tRNA promoterAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-Twin-Strep_IRES-mRuby2
Plasmid#113885PurposeMammalian (inducible) protein expression, bicistronic expression of cytosolic mRuby2DepositorTypeEmpty backboneUseLentiviralTags3C-Twin-StrepPromoterCMV-MIE-TetO2Available sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET PI-SceI C-His K403A
Plasmid#195612PurposeExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K403A active site mutationDepositorInsertPI-SceI
ExpressionBacterialMutationA1463G, A1464CPromoterT7Available sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-Q22YU3-2xStrep
Plasmid#170315PurposePlasmid for insect cell expression of Tetrahymena thermophila Q22YU3 C-terminally tagged with 2x Strep-tag IIDepositorInsertQ22YU3 (TTHERM_00122270 Tetrahymena thermophila)
TagsStrep-Tag IIExpressionInsectPromoterPolyhedrinAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-Q22MS1-2xStrep
Plasmid#170314PurposePlasmid for insect cell expression of Tetrahymena thermophila Q22MS1 C-terminally tagged with 2x Strep-tag IIDepositorInsertQ22MS1
TagsStrep-Tag IIExpressionInsectPromoterPolyhedrinAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP-Leu2d-GgKDELR2 R159K
Plasmid#123626PurposeExpresses GgKDELR2 with Arg159Lys mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selectionDepositorAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP-Leu2d-GgKDELR2 E127Q
Plasmid#123623PurposeExpresses GgKDELR2 with Glu127Gln mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selectionDepositorAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP-Leu2d-GgKDELR2 Y158F
Plasmid#123624PurposeExpresses GgKDELR2 with Tyr158Phe mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selectionDepositorAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP-Leu2d-GgKDELR2 R159A
Plasmid#123625PurposeExpresses GgKDELR2 with Arg159Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selectionDepositorAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP-Leu2d-GgKDELR2 H12A
Plasmid#123619PurposeExpresses GgKDELR2 with His12Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selectionDepositorAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP-Leu2d-GgKDELR2 R47A
Plasmid#123620PurposeExpresses GgKDELR2 with Arg47Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selectionDepositorAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only