We narrowed to 11,622 results for: VARS

1. pHis17_ParM _2

   Plasmid

   #78202

   Purpose

   Expression of double-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor

   Depositor

   Martin Webb

   Article

   Kunzelmann et al ACS Chem Biol. 2010 Apr 16;5(4):415-25.

   Insert

   parM (K33A D63C T174A T175N D224C C287A)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   K33A D63C T174A T175N D224C C287A

   Promoter

   unknown

   Available Since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR_CMV_MeltNLS/NES-40-mCherry

   Plasmid

   #234066

   Purpose

   Encodes a protein that localizes to nucleus under the control of a Melt variant with a switching temperature of 40°C

   Depositor

   Lukasz Bugaj

   Article

   Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

   Insert

   MeltNLS/NES-40-mCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDH_Str-Ii

   Plasmid

   #65313

   Purpose

   Cytoplasmic ER hook only (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Invariant chain (p33)
   

   Use

   Lentiviral

   Mutation

   Streptavidin in its cytoplasmic part

   Promoter

   CMV

   Available Since

   Aug. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR hH1-TERRA

   Plasmid

   #111381

   Purpose

   TERRA with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences under control of hH1 promoter and terminator

   Depositor

   Klaus Holzmann

   Article

   Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

   Insert

   hH1-TERRA
   

   Use

   Gateway entry cloning

   Promoter

   hH1

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. mEmerald-ERBB2-N-18

   Plasmid

   #62755

   Purpose

   Localization: Membrane Receptor, N1 Cloning Vector, Excitation:487, Emission:509

   Depositor

   Michael Davidson

   Insert

   ERBB2 (ERBB2 Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   March 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pAAV-Ef1a-fDIO-GCaMP6s

   Plasmid

   #105714

   Purpose

   Can be used to express GCaMP6s in the presence of Flp recombinase. Can be used to create adeno-associated virus to express Flp-dependent GCaMP6s.

   Depositor

   Rylan Larsen

   Has Service

   AAV8

   Insert

   GCaMP6s
   

   Use

   AAV; Flp/frt

   Expression

   Mammalian

   Promoter

   Ef1a, Human elongation factor-1 alpha

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. eNme2C-CBE6b

   Plasmid

   #215830

   Purpose

   Express CBE6b (with eNme2-C variant) in mammalian cells

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6b-eNme2C-2xUGI
   

   Expression

   Mammalian

   Available Since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pH-tdGFP

   Plasmid

   #74322

   Purpose

   pH stable tandem dimeric sfGFP variant (N149Y,Q204H) in pFA6a-URA3MX. Codon optimized for S. cerevisiae

   Depositor

   Joerg Stelling

   Article

   Roberts et al Sci Rep. 2016 Jun 21;6:28166. doi: 10.10

   Insert

   pH-tdGFP
   

   Use

   Yeast tagging

   Tags

   none

   Expression

   Yeast

   Mutation

   N149Y, Q204H

   Available Since

   May 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-6xHis-GFP(-30)

   Plasmid

   #199167

   Purpose

   used to express a supercharged GFP variant in E. coli

   Depositor

   Allie Obermeyer

   Article

   Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

   Insert

   GFP(-30)
   

   Tags

   MGHHHHHHGGA

   Mutation

   T19D, K36E, N49E, K51E, K62E, R83D, K89D, N159D, …

   Promoter

   T7

   Available Since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL0_20 [panARS-opt ORI]

    Plasmid

    #198933

    Purpose

    Level 0 part

    Depositor

    Charlie Gilbert, Nili Ostrov, Cultivarium Tools

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    panARS-opt ORI
    

    Use

    Synthetic Biology

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-ND1 Left -GSVG-UGI

    Plasmid

    #187412

    Purpose

    human mitochondrial DNA ND1 target TALE

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

    Insert

    DddA GSVG variant
    

    Expression

    Mammalian

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHis17_ParM _1

    Plasmid

    #78201

    Purpose

    Expression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor

    Depositor

    Martin Webb

    Article

    Kunzelmann et al J Biol Chem. 2009 Nov 27;284(48):33130-8

    Insert

    parM (K33A I27C T174A T175N C287A)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    K33A I27C T174A T175N C287A

    Promoter

    unknown

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST14-DoubleCatcher-H-Lock

    Plasmid

    #216285

    Purpose

    Expresses DoubleCatcher H-lock in bacterial cells. DoubleCatcher H-lock is a variant of DoubleCatcher in which each SpyCatcher003 moiety is separated by an alpha helical linker.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher H-Lock
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-CitronRH

    Plasmid

    #158545

    Purpose

    Mammalian expression of nonbinding variant of direct-response citrate sensor

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    CitronRH
    

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. eNme2C-CBE6c

    Plasmid

    #215831

    Purpose

    Express CBE6c (with eNme2-C variant) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6c-eNme2C-2xUGI
    

    Expression

    Mammalian

    Available Since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJH462

    Plasmid

    #172154

    Purpose

    pylBCD variant in pBAD backbone

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Para (Sumo-tagged pylBCD); AraC
    

    Expression

    Bacterial

    Mutation

    SUMO tag fused to N-terminus of PylB

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJH643

    Plasmid

    #172168

    Purpose

    Reporter plasmid for pylBCD variant in pBAD backbone

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Para sfGFP.3TAG; Plpp pylS; PproK pylT; AraC; Cam
    

    Expression

    Bacterial

    Mutation

    sfGFP(N39*, N135*, Y151*)

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. p458 VQR

    Plasmid

    #101727

    Purpose

    Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Expression

    Mammalian

    Mutation

    VQR

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL2_163

    Plasmid

    #199095

    Purpose

    Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

    Depositor

    Charlie Gilbert, Nili Ostrov, Cultivarium Tools

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    pAMβ1 ORI plus NGS barcode 3, gentamicin resistance cassette, mScarlet-I cassette, oriT
    

    Use

    Synthetic Biology

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. mFGF8b

    Plasmid

    #22090

    Depositor

    Gail Martin

    Article

    Crossley et al Development. 1995 Feb . 121(2):439-51.

    Insert

    FGF8 (Fgf8 Mouse)
    

    Use

    Bacterial

    Available Since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only