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pscMS2(WTx2)-EGFP

Plasmid

#140258

Purpose

MS2CP-dependent translational repression

Depositor

Hirohide Saito

Article

Ono et al ACS Synth Biol. 2020 Jan 17;9(1):169-174

Insert

Two MS2CP-binding sites embedded in scaffold

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-PIG-METTL14-R298P

Plasmid

#141119

Purpose

Retroviral overexpression of mutant METTL14 in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

METTL14 (METTL14 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Substitution - Missense, position 298, R➞P

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBABE-RPS14(WT)-Myc

Plasmid

#122235

Purpose

expresses RPS14 protein with a Myc tag in mammalian cells (puro resistance)

Depositor

Gerardo Ferbeyre

Article

Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

Insert

RPS14 (RPS14 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

vector's LTR

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/NS5-Af-mut

Plasmid

#120829

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

ZIKV NS5 V114M/V872M - "african mut"

Tags

V5

Expression

Mammalian

Mutation

V114M/V872M

Promoter

CMV

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pSUPER-hHSF2 shRNA

Plasmid

#118346

Purpose

This plasmid expresses shRNA against hHSF2.

Depositor

Lea Sistonen

Article

Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

Insert

hHSF2 shRNA (HSF2 Human)

Expression

Mammalian

Mutation

WT

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pFL-SV40-p38 β/MAPK11 5'-UTR

Plasmid

#115357

Purpose

firefly luciferase (Fluc) reporter containing the 5’-UTR of p38 β/MAPK11 mRNA

Depositor

Ming-Chih Lai

Article

Ku et al Mol Cell Biol. 2018 Dec 11;39(1). pii: M

Insert

p38 β/MAPK11 5'-UTR (MAPK11 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV2-TBG-FLAG-mRIPK1-S321A

Plasmid

#115342

Purpose

Liver-specific adeno-associated viral delivery and mammalian expression of Flag-mRIPK1-S321A

Depositor

Junying Yuan

Article

Geng et al Nat Commun. 2017 Aug 25;8(1):359. doi: 1

Insert

RIPK1 (Ripk1 Mouse)

Use

AAV

Tags

Flag

Expression

Mammalian

Mutation

S321A

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-LAT Y6F YYYY-LAT YYYY -mCitrine (XSB588)

Plasmid

#78516

Purpose

Lentiviral vector for expressing LAT-Y6F YYYY-LAT-YYYY-mCitrine (3x2 Grb2 binding sites)

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

LAT (LAT Human)

Use

Lentiviral

Tags

mCitrine

Expression

Mammalian

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pLEX307-mFzd3

Plasmid

#102865

Purpose

Expresses mouse Fzd3 in mammalian cells

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Frizzled 3 (Fzd3 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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myc-hIFITM3-delta1-21

Plasmid

#104365

Purpose

Expresses human IFITM3-delta1-21 with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al PLoS Pathog. 2015 Aug 11;11(8):e1005095.

Insert

IFITM3 (IFITM3 Human)

Tags

myc

Expression

Mammalian

Mutation

deleted amino acids 1-21

Promoter

CMV

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S831E

Plasmid

#91997

Purpose

Expresses FLAG-HA-AGO2 (S831E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S831E

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 (+) Tacδ

Plasmid

#81177

Purpose

Mammalian expression of human Tacδ (GFFKR cytoplasmic tail)

Depositor

Chinten James Lim

Article

Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

Insert

Tacδ

Tags

KLGFFKR

Expression

Mammalian

Mutation

CD25 amino acids 1 to 263 fused to KLGFFKR cytopl…

Promoter

CMV

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/SH/GW-Mst1

Plasmid

#84299

Purpose

Expresses a tetracycline-inducible SH-tagged (streptavidin-binding peptide and HA tag) version of Mst1. Used in conjunction with Flp-In cells to generate single copy transgene.

Depositor

Jonathan Chernoff

Article

Rawat et al J Biol Chem. 2013 Mar 22;288(12):8762-71

Insert

Mst1 (STK4 Human)

Tags

HA, SBP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2016

Availability

Academic Institutions and Nonprofits only
pMIR-31-Reporter

Plasmid

#71871

Purpose

Mammalian expression vector for the analysis of miR-31 activity

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

Reverse complementary sequence of miR-31

Tags

firefly luciferase

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pEGB2alpha2 35s:hCas9:tNos (GB0639)

Plasmid

#68222

Purpose

Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

hCas9

Use

CRISPR and Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pHMWGFPR

Plasmid

#64396

Purpose

High molecular weight glutenin gene promoter driving GFP expression

Depositor

Claudia Vickers

Article

Vickers et al Plant Mol Biol. 2006 Sep;62(1-2):195-214

Insert

High molecular weight glutenin gene promoter

Tags

GFP

Expression

Plant

Promoter

High molecular weight glutenin gene promoter

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only
pGS1011

Plasmid

#63783

Purpose

Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Strep tag II followed by TRP1 selection marker

Depositor

Monika Golas, Bjoern Sander

Article

Rai et al Mol Biotechnol. 2014 Jun 27.

Insert

Strep-tag II followed by TRP1 selection marker

Use

E. coli cloning vector

Tags

Strep-tag II

Promoter

none

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-Gm129

Plasmid

#53765

Purpose

mammalian expression of EGFP-Gm129

Depositor

Aziz Sancar

Article

Annayev et al J Biol Chem. 2014 Feb 21;289(8):5013-24.

Insert

Gm129 (Ciart Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

*

Promoter

CMV

Available Since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only
pMXs-hs-3xHA-7CtoA-LIN-41

Plasmid

#52726

Purpose

retroviral expression of human LIN-41 / TRIM71 containing cysteine to alanine point mutations of all 7 cysteines in the RING domain and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

changed cysteines 12, 15, 61, 66, 69, 91, and 94 …

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pGFH14A6-12

Plasmid

#39543

Depositor

Katharina Strub

Article

Lakkaraju et al Cell. 2008 May 2;133(3):440-51.

Insert

SRP14 (SRP14 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 101-113

Promoter

CMV

Available Since

Sept. 14, 2012

Availability

Academic Institutions and Nonprofits only