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minitalin (headR13DD)

Plasmid

#182994

Purpose

expression of minitalin wt in mammalian cells

Depositor

Vesa Hytönen

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

minitalin (Tln1 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLib6.6B

Plasmid

#176666

Purpose

Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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MTK2_013

Plasmid

#123705

Purpose

Encodes a spacer (for multicistronic) as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pSpacer multicistronic

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pBTK206

Plasmid

#110588

Purpose

BTK Type 3 coding sequence for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

Nanoluc

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLENTI_RPS3_K227/230R

Plasmid

#127135

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3 (RPS3 Human)

Use

Lentiviral

Mutation

K227R/K230R

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS3_K230R

Plasmid

#127134

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3 (RPS3 Human)

Use

Lentiviral

Mutation

K230R

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS3_K227R

Plasmid

#127133

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3 (RPS3 Human)

Use

Lentiviral

Mutation

K227R

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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HsCERK

Plasmid

#118600

Purpose

Expresses ceramide kinase, which catalyzes the phosphorylation of ceramide to form ceramide 1-phosphate

Depositor

Kevin Lynch

Article

Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

Insert

Homo sapiens ceramide kinase

Tags

FLAG

Expression

Yeast

Promoter

GAL1, 10

Available Since

Feb. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pYZ_3WJrep_N10_trigger

Plasmid

#132728

Purpose

T7 RNAP-driven expression of 3WJ repressor index 10 trigger RNA

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_N10_trigger

Expression

Bacterial

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ_3WJrep_N10_switch

Plasmid

#132724

Purpose

T7 RNAP-driven expression of 3WJ repressor index 10 switch RNA with a GFP-ASV reporter

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_N10_switch

Expression

Bacterial

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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V5-WT-short-UBASH3A

Plasmid

#192088

Purpose

Mammalian expression of human wild-type short-form UBASH3A with V5 tag

Depositor

Patrick Concannon

Article

Ge et al Diabetes. 2017 Jul;66(7):2033-2043. doi:

Insert

UBASH3A (UBASH3A Human)

Tags

V5

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0160

Plasmid

#172812

Purpose

Level 1 module containing P19_P1 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CDS1_P19-3U+TERNOS

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pOpLexA::mini35s (GB0733)

Plasmid

#68180

Purpose

Provides the 4xLexA Operator fused to CaMV 35s minimal promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

4xOpLexA:mini35S

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pETM6-Vv4CL-PhCHS-CmCHI

Plasmid

#73401

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Vv4CL
PhCHS
CmCHI

Expression

Bacterial

Promoter

T7

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ66

Plasmid

#24981

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2010 Jun;7(6):451-3. doi: 1

Insert

Cbr-unc-119 Punc-122::GFP

Use

Multisite gateway

Available Since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pGW-FL-V5

Plasmid

#78858

Purpose

DULIP Prey Destination Vector (Gateway, C-terminal)

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Type

Empty backbone

Use

Gateway destination vector

Tags

Firefly luciferase, V5-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pGW-RL-PA

Plasmid

#78856

Purpose

DULIP Bait Destination Vector (Gateway, C-terminal)

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Type

Empty backbone

Use

Gateway destination vector

Tags

Renilla luciferase, Protein A

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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GST-SmB

Plasmid

#1841

Depositor

Philip Leder

Article

Bedford et al Proc Natl Acad Sci U S A 1998 Sep 1;95(1

Insert

SmB (SNRPB Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
pResQ shDna2'

Plasmid

#31952

Depositor

Sheila Stewart

Article

Duxin et al Mol Cell Biol. 2009 Aug;29(15):4274-82.

Insert

shRNA against hDna2 (DNA2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pmCellFree_KA1_ZIKV PrM

Plasmid

#145340

Purpose

Cell-free/mammalian expression vector for Zika virus PrM protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV PrM

Tags

8xHis and eGFP

Expression

Bacterial

Promoter

T7- CMV/CAG

Available Since

April 29, 2023

Availability

Academic Institutions and Nonprofits only

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