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pGL3-U6-sgRNA-PGK-puromycin-AflII

Plasmid

#106404

Purpose

Empty guide RNA cloning vector with an AflII site added from Addgene 41824

Depositor

Jason Gertz

Article

Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.

Type

Empty backbone

Expression

Mammalian

Promoter

U6

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-T7-hAIM2

Plasmid

#51541

Purpose

Express T7 and GFP-tagged hAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Tags

GFP and T7

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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7iBi89 (rat fibronectin)

Plasmid

#14065

Depositor

Richard Hynes

Article

Huh et al Mol Cell Biol. 1993 Sep . 13(9):5301-14.

Insert

Rat Fibronectin 7iBi89 (Fn1 Rat)

Expression

Mammalian

Mutation

Insert was cut out of pGEM3 with SalI and SacI an…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
p3A9cbetaTCR.HSV2.3

Plasmid

#69594

Purpose

TCR beta chain genomic transgenic construct for generation of gBT-I mice

Depositor

Francis R. Carbone

Article

Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

Insert

TCR beta VJ region from clone HSV2.3 (Tcrb Mouse)

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only
pIVEX2.3 TEM-8

Plasmid

#16563

Depositor

Bert Vogelstein

Article

Carson-Walter et al Cancer Res. 2001 Sep 15. 61(18):6649-55.

Insert

TEM8 (ANTXR1 Human)

Expression

Bacterial

Mutation

Extracellular region

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
pLA CMV N-HA RALB WT

Plasmid

#50989

Purpose

Expresses RALB in mammalian cells

Depositor

Anna Sablina

Article

Simicek et al Nat Cell Biol. 2013 Oct;15(10):1220-30.

Insert

RALB (RALB Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only
RASD2

Plasmid

#55672

Purpose

expression of mouse RASD2

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RASD2 (Rasd2 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
p1DT (chicken beta 1 integrin)

Plasmid

#14066

Depositor

Richard Hynes

Article

Tamkun et al Cell. 1986 Jul 18. 46(2):271-82.

Insert

Chicken Beta 1 Integrin (ITGB1 Chicken)

Expression

Mammalian

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

Plasmid

#101107

Purpose

Encodes dCas9(C)-VP64 with SAM components fused to BDKRB2

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

BDKRB2-dCas9(N)

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLN429 (FuGW-G8p-mKate2)

Plasmid

#105185

Purpose

Module 3 - GAL4 synthetic promoter ( with eight binding sites) drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/FLAGHA-SCAF8

Plasmid

#122470

Purpose

Dox-inducible FLAG/HA (N-terminal tag) SCAF8 expression

Depositor

Jesper Svejstrup

Article

Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

Insert

SCAF8 isoform C CRISPR resistant (SCAF8 Human)

Tags

FLAGHA

Expression

Mammalian

Mutation

Synonymous mutations conferring resistance to gRN…

Promoter

CMV

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-tLuc

Plasmid

#107552

Purpose

tLuc in AAV backbone

Depositor

Juan Belmonte

Article

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Insert

Luciferase

Use

AAV

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pTK_Citrine_Nanotag_5_neg_Control

Plasmid

#130558

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

negative oligo sequence

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pXWJ109As-gfp

Plasmid

#123143

Purpose

An arsenic sensor with optimized intracellular receptor density. pSB3K3 carrying J109-30arsR-t-ParsR-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J109-30arsR-t-ParsR-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J109, ParsR

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-MKRN1 WT

Plasmid

#78766

Purpose

To detect localization of MKRN1 in live mammalian cells

Depositor

Jaewhan Song

Article

Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

Insert

MKRN1 (MKRN1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pT2-cryR;clecl14aCE1-P1Egfp

Plasmid

#90157

Purpose

contains clec14a enhancer sequence, and clec14a specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

clec14aE1P1Egfp

Tags

EGFP

Expression

Bacterial

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_D27NDHFR_WTTS

Plasmid

#91244

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

D27N

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_F31YG121VDHFR_WTTS

Plasmid

#91229

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

F31YG121V

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_G121VDHFR_WTTS

Plasmid

#91233

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

G121V

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_K1087A

Plasmid

#91864

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbuCas13a (LbuC2c2) K1087A

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

K1087A

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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