167,948 results

1. pBGPa-CMV-GFP-OSBP PH domain

   Plasmid

   #58724

   Purpose

   Expresses GFP-Tagged OSBP PH domain in mammalian cells

   Depositor

   Tim Levine

   Article

   Levine et al Curr Biol. 2002 Apr 30;12(9):695-704.

   Insert

   Oxysterol-binding Protein PH domain (OSBP Human)
   

   Tags

   GFP and myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

2. USP15

   Plasmid

   #28282

   Depositor

   Cheryl Arrowsmith

   Insert

   USP15 (USP15 Human)
   

   Tags

   His tag with thrombin cleavage

   Expression

   Bacterial

   Mutation

   Contains dusp and Ubl domains (residues 6-223).

   Available Since

   April 14, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pFUW-tetO-L-MYC

   Plasmid

   #139817

   Purpose

   doxycycline-inducible expression of Human L-MYC in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   L-MYC (MYCL Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE-mCMV

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEff

   Plasmid

   #97423

   Purpose

   Transient expression of recombinant proteins in plants. The plasmid contains gfp, which is replaced by the gene of interest.

   Depositor

   Nikolai Ravin

   Article

   Mardanova et al Front Plant Sci. 2017 Feb 28;8:247. doi:

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2+ H2B-mTagBFP2

   Plasmid

   #99267

   Purpose

   Contains a fusion of human histone H2B to fluorescent protein mTagBFP2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

   Depositor

   Philipp Keller

   Insert

   mTagBFP2-3XHA
   

   Tags

   H2B

   Expression

   Mammalian

   Promoter

   SP6

   Available Since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG PSAM4 GlyR IRES EGFP

   Plasmid

   #119739

   Purpose

   Chemogenetic inhibitor expression plasmid

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2019 Mar 14. pii: science.aav52

   Insert

   PSAM4 GlyR IRES eGFP (CHRNA7 Synthetic, Human)
   

   Expression

   Mammalian

   Mutation

   L131G, Q139L, Y217F

   Promoter

   CAG

   Available Since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCB-hDot1Lmut

   Plasmid

   #74174

   Purpose

   For packaging hDot1Lmut (catalytic activity dead) to retrovirus.

   Depositor

   Yi Zhang

   Article

   Okada et al Cell. 2005 Apr 22;121(2):167-78.

   Insert

   hDot1L (DOT1L Human)
   

   Use

   Retroviral

   Tags

   HA

   Mutation

   hDot1L 163 GSG to RCR mutation*

   Available Since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pMRX-IZ-ANXA11-mRuby3

   Plasmid

   #184894

   Purpose

   Stable expression of ANXA11-mRuby3 (Annexin A11-mRuby3) in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

   Insert

   ANXA11 (ANXA11 Human)
   

   Tags

   mRuby3

   Expression

   Mammalian

   Mutation

   R230C relative to NP_001148.1

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. sg_Trp53_i3-ipUSEPR-TR657

   Plasmid

   #228929

   Purpose

   Knockdown of Trp53 in mammalian cells

   Depositor

   Sika Zheng

   Article

   Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

   Insert

   sg_Trp53_i3 (Trp53 Mouse)
   

   Use

   Lentiviral

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Gamillus/ pcDNA3

    Plasmid

    #124837

    Purpose

    mammalian expression of Gamillus, an acid-tolerant monomeric green fluorescent protein

    Depositor

    Takeharu Nagai

    Article

    Shinoda et al Cell Chem Biol. 2018 Mar 15;25(3):330-33

    Insert

    Gamillus
    

    Tags

    No tag

    Expression

    Mammalian

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-C-mStayGold-EFS-Blast

    Plasmid

    #227257

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-ASH1L

    Plasmid

    #65369

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    ASH1L (ASH1L Human)
    

    Tags

    EmGFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. SIN40C.TRE.MCS.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

    Plasmid

    #169283

    Purpose

    Doxycycline-inducible overexpression of cDNA with dTomato as a reporter fluorescent protein

    Depositor

    Dirk Heckl

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. HIV-1-GFP ΔEnv

    Plasmid

    #217437

    Purpose

    HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu.

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Insert

    gag/pol
    

    Use

    Lentiviral

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBbAk-w4-loxP-TT-loxP-mCherry-PCB

    Plasmid

    #228469

    Purpose

    Fluorescent reporter for Cre recombinase activity and the PCB production gene. Cre removes a transcription terminator, leading to mCherry production

    Depositor

    Mary Dunlop

    Article

    Jafarbeglou et al ACS Synth Biol. 2024 Nov 19. doi: 10.102

    Inserts

    w4-loxP-TT-loxP-mCherry
    Ho1
    PcyA
    

    Expression

    Bacterial

    Promoter

    J23106, J23108, and T7A1

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA-Foxo1ADA (pCMV5)

    Plasmid

    #12143

    Depositor

    Domenico Accili

    Article

    Nakae et al J Clin Invest. 2001 Nov . 108(9):1359-67

    Insert

    Foxo1 ADA (Foxo1 Mouse)
    

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Phosphorylation-deficient, also referred to as co…

    Promoter

    CMV

    Available Since

    Aug. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti.CAG.H2B-cerFP.W.SINloxP

    Plasmid

    #51006

    Purpose

    Lentivector that expresses H2B-cerFP from internal CAG enhancer-promoter

    Depositor

    Rusty Lansford

    Insert

    H2B-cerFP
    

    Use

    Lentiviral

    Tags

    histone 2B

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-UCOE-EF1a-Zim3-dCas9-P2A-GFP

    Plasmid

    #188778

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Promoter

    EF1a

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5 FRT TO GFP11-LRRK2_G2019S

    Plasmid

    #231175

    Purpose

    Expresses GFP-11-LRRK2 G2019S in mammalian cells

    Depositor

    Samara Reck-Peterson

    Article

    Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

    Insert

    GFP11-LRRK2 (G2019S) (LRRK2 Human)
    

    Tags

    GFP11

    Expression

    Mammalian

    Mutation

    G2019S

    Promoter

    CMV

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDD104 (Peft-3::Cre)

    Plasmid

    #47551

    Purpose

    Cre recombinase expression plasmid for removal of floxed selectable markers in the C. elegans germline

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

    Insert

    Cre Recombinase
    

    Use

    Cre/Lox

    Expression

    Worm

    Promoter

    eef-1A.1 (eft-3)

    Available Since

    Sept. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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