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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,474 results for: shi

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Showing: 9541 - 9560 of 14474 results

  4. pAV10.KH

    Plasmid
    #63208
    Purpose
    pCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    63208-plasmid-map-sequence-id-104909

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  10. pHLmMBP-2

    Plasmid
    #72344
    Purpose
    Mammalian expression of secreted N-terminally 6His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Article
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Tags
    6His-tag, HA-tag, Strep-tag II, and mMBP
    Expression
    Mammalian
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Available Since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    72344-plasmid-map-sequence-id-132067

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  13. pCRISPEY-GAL-Nat-ADE2

    Plasmid
    #232103
    Purpose
    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.
    Depositor
    Jeffrey Lewis
    Article
    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:
    Insert
    ADE2 gRNA and repair template
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Repair template introduces C at nt 466 of ADE2 to…
    Promoter
    GAL7
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232103-plasmid-map-sequence-id-461835

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  20. pTon1(acgfp1)

    Plasmid
    #233648
    Purpose
    Expression vector of AcGFP1 under control of tetracycline responsive element (TRE). Also express Tet-on advenced transactivator (rtTA) under control of minimal CMV promoter.
    Depositor
    Takeo Kubo
    Article
    Tsujioka et al Nat Commun. 2017 Sep 8;8(1):495. doi: 10
    Insert
    target sequence of guide RNA #tyr (3253-3275) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'
    Use
    Amphibian expression, tet-on
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    233648-plasmid-map-sequence-id-468211

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Showing: 9541 - 9560 of 14474 results