We narrowed to 166,989 results for: Gene

1. SUN2delSUNec

   Plasmid

   #130847

   Purpose

   Expression of plant LINC fluorescent fusion protein for cell biology

   Depositor

   Hank Bass

   Article

   Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

   Insert

   SUN2
   

   Use

   Gateway entry vector

   Tags

   mCherry-FLAG-HA

   Mutation

   Deletion of SUN domain

   Availability

   Academic Institutions and Nonprofits only

   

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2. SUN2delCCec

   Plasmid

   #130848

   Purpose

   Expression of plant LINC fluorescent fusion protein for cell biology

   Depositor

   Hank Bass

   Article

   Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

   Insert

   SUN2
   

   Use

   Gateway entry vector

   Tags

   mCherry-FLAG-HA

   Mutation

   Deletion of coiled coil domain

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cdk12_intron3_sg2_pX330

   Plasmid

   #127174

   Purpose

   sgRNA that cuts within intron 3 of mouse Cdk12 genomic locus in pX330 backbone

   Depositor

   Phil Sharp

   Article

   Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

   Insert

   Mouse Cdk12 Intron 3 sgRNA
   

   Use

   CRISPR and Mouse Targeting

   Promoter

   U6 Promoter

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cdk12_intron4_sg1_pX458

   Plasmid

   #127175

   Purpose

   sgRNA that cuts within intron 4 of mouse CDK12 genomic locus in pX458 backbone

   Depositor

   Phil Sharp

   Article

   Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

   Insert

   Mouse Cdk12 Intron 4 sgRNA
   

   Use

   CRISPR and Mouse Targeting

   Promoter

   U6 Promoter

   Availability

   Academic Institutions and Nonprofits only

   

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5. CaMCLC-His

   Plasmid

   #111292

   Purpose

   bacterial expression

   Depositor

   Vamsi Mootha

   Article

   Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

   Insert

   (S)-malyl-CoA/b-methylmalyl-CoA/(S)-citramalyl- CoA lyase
   

   Tags

   His tag, GGGSGGGSLEHHHHHH

   Expression

   Bacterial

   Availability

   Academic Institutions and Nonprofits only

   

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6. MsMUT_sgRNA1

   Plasmid

   #111296

   Purpose

   CRISPR KO murine MUT

   Depositor

   Vamsi Mootha

   Article

   Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

   Insert

   sgRNA1 against murine MUT
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Availability

   Academic Institutions and Nonprofits only

   

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7. MsMUT_sgRNA3

   Plasmid

   #111297

   Purpose

   CRISPR KO murine MUT

   Depositor

   Vamsi Mootha

   Article

   Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

   Insert

   sgRNA3 against murine MUT
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-sgCrebbp#2/Cre

   Plasmid

   #193210

   Purpose

   Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Crebbp gene

   Depositor

   Julien Sage

   Article

   Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

   Insert

   sgCrebbp#2 (Crebbp Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-dCas9-p300 Core

   Plasmid

   #61357

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   CMV

   Available Since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-dCas9-p300 Core (D1399Y)

    Plasmid

    #61358

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…

    Promoter

    CMV

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid

    #196084

    Purpose

    (Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Inserts

    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Catalytically dead mutant of the Cas9 endonucleas…

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-dCas9-p300 Core (Y1467F)

    Plasmid

    #61362

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inavtivating mutation Y1467F) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; Y1467F mutation i…

    Promoter

    CMV

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid

    #196078

    Purpose

    (Empty Backbone) Constitutive CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Inserts

    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    

    Expression

    Mammalian

    Mutation

    Catalytically dead mutant of the Cas9 endonucleas…

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-U6-sgRNA(CTRL)-CMV-eGFP

    Plasmid

    #194017

    Purpose

    Expresses a gRNA that targets the LacZ gene (serves as control) and eGFP

    Depositor

    Larry Young

    Article

    Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

    Inserts

    sgRNA(CTRL)
    eGFP
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV phSyn1(S)-FlpO-bGHpA

    Plasmid

    #51669

    Purpose

    Can be used to generate AAV virus that will express the FlpO recombinase gene in neurons from the synapsin promoter.

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Has Service

    AAV Retrograde

    Insert

    FlpO recombinase gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn1

    Available Since

    May 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPaint-mScarlet-PuroR [M1G]

    Plasmid

    #171809

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-T2A-PuroR
    

    Use

    Gene tagging

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPaint-mScarlet-BlastR [M1G]

    Plasmid

    #171814

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and blasticidin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-T2A-BlastR
    

    Use

    Gene tagging

    Mutation

    BlastR: Changed Methionin 1 to Glycine.

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDGB1alpha2_KanR_BastaR_Rb7_GFP

    Plasmid

    #186427

    Purpose

    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar) with fluorescent marker (eGFP)

    Depositor

    Lukas Fischer

    Article

    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

    Inserts

    KanR
    BastaR
    Rb7
    eGFP
    

    Use

    Synthetic Biology; Binary vector for escherichia …

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    CaMV 35S and Pnos

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-sgCrebbp#1/Cre

    Plasmid

    #193209

    Purpose

    Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Crebbp gene

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    sgCrebbp#1 (Crebbp Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCRISPaint-mNeon-PuroR [M1G]

    Plasmid

    #171800

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-T2A-PuroR
    

    Use

    Gene tagging

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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