Addgene

We narrowed to 2,080 results for: 3-Oct

Showing: 941 - 960 of 2080 results

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pISH-Six1

Plasmid

#74358

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Six1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

sine oculis-related homeobox 1 (Six1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pSrpR-BCD20-Cerulean-ssrA P65U23C89T19 v50-2a (587)

Plasmid

#70744

Purpose

for building 3-color reporter plasmid

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

Cerulean-ssrA

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pME18S-12053

Plasmid

#55603

Purpose

Aedes aegypti NPY-like receptor AAEL012053 (NPYLR3) full-length clone in mammalian expression vector

Depositor

Leslie Vosshall

Article

Liesch et al PLoS Negl Trop Dis. 2013 Oct 10;7(10):e2

Insert

NPYLR3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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psiCheck2 6xmut UTR

Plasmid

#40764

Purpose

Renilla luciferase reporter containing siRNA target sites

Depositor

Phillip Zamore

Article

Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

Insert

6 copies (3 site expanded) of the CXCR4 small RNA target site

Use

Luciferase

Tags

luciferase

Expression

Mutation

Promoter

TK/SV40

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
Hox B8b

Plasmid

#36465

Depositor

Robert Ho

Article

Ahn et al Dev Biol. 2008 Oct 1;322(1):220-33. Epub

Insert

hoxB8b (hoxb8b Zebrafish)

Use

Riboprobe synthesis

Tags

Expression

Mutation

Promoter

T7

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
Hox A9b

Plasmid

#36464

Depositor

Robert Ho

Article

Ahn et al Dev Biol. 2008 Oct 1;322(1):220-33. Epub

Insert

hoxA9b (hoxa9b Zebrafish)

Use

Riboprobe synthesis

Tags

Expression

Mutation

S46G

Promoter

T7

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA

Plasmid

#104095

Purpose

Expresses TREAT-siRNA reporter in mammalian cells

Depositor

Jeffrey Chao

Article

Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

Insert

Renilla luciferase

Use

Tags

24xMS2 stem loops in 3'UTR, 24xPP7 stem loop…

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pSF4 TetCMV intron Renilla STOP 12xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA

Plasmid

#104094

Purpose

Expresses TREAT reporter in mammalian cells

Depositor

Jeffrey Chao

Article

Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

Insert

Renilla luciferase

Use

Tags

12xPP7 stem loops in 3'UTR, 24xMS2 stem loop…

Expression

Mammalian

Mutation

Promoter

TetCMV

Available since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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rAAV2-retro helper

Plasmid

#81070

Purpose

retrograde AAV helper vector, carrying rep and modified capsid gene

Depositor

David Schaffer, Alla Karpova

Article

Tervo et al Neuron. 2016 Oct 6. pii: S0896-6273(16)3

Inserts

rep
rAAV2-retro cap

Use

AAV and Unspecified; Helper vector

Tags

Expression

Mutation

Pro2Ala, possibly Asp613Gly and changes made to A…

Promoter

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pEF-Bos TRAM Flag

Plasmid

#41551

Depositor

Doug Golenbock, Kate Fitzgerald

Article

Fitzgerald et al J Exp Med. 2003 Oct 6;198(7):1043-55. Ep

Insert

TRAM (TICAM2 Human)

Use

Tags

6xHis and Flag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 14, 2012

Availability

Academic Institutions and Nonprofits only
pMXs-hM₃O-IP

Plasmid

#46645

Depositor

Nobuaki Kikyo

Article

Hirai et al Stem Cells. 2011 Sep;29(9):1349-61. doi:

Insert

hM₃O (POU5F1 Human)

Use

Retroviral

Tags

IRES Puro and human MyoD (aa 1-62)

Expression

Mammalian

Mutation

contains a fragment of human MyoD (aa1-62) fused …

Promoter

Available since

July 22, 2013

Availability

Academic Institutions and Nonprofits only

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C1ORF43-GFP

Plasmid

#130879

Purpose

Expresses human C1ORF43 with C-terminal GFP tag

Depositor

Michael Bassik

Article

Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

Insert

C1ORF43 (C1orf43 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA4TO K164A H165A CoV-2 nsp1 3xFLAG

Plasmid

#176060

Purpose

Expresses K164A K165A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV-2 K164A H165A nsp1 (ORF1ab SARS-CoV-2)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

K164A and H165A

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA4TO R124A K125A CoV-2 nsp1 3xFLAG

Plasmid

#176059

Purpose

Expressing R124A K125A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV-2 nsp1 R124A/K125A (ORF1ab SARS-CoV-2)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

R124A and K125A

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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C1ORF43-3xFLAG

Plasmid

#130880

Purpose

Expresses human C1ORF43 with C-terminal 3xFLAG tag

Depositor

Michael Bassik

Article

Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

Insert

C1ORF43 (C1orf43 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA4 h-Stx3 resistant to shRNA #304-2xMyc/His

Plasmid

#99746

Purpose

Expresses human Stx3 with a C-term myc-myc-his that is resistant to shRNA #304 from Sigma Aldrich.

Depositor

Thomas Weimbs

Article

Giovannone et al Mol Biol Cell. 2017 Oct 15;28(21):2843-2

Insert

Stx3 (STX3 Human)

Use

Tags

myc-myc-his

Expression

Mammalian

Mutation

Made six (6) silent mutations to confer resistanc…

Promoter

CMV/TO

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCVL.SSA TLR (CCR5 TALEN Sce Spacer)

Plasmid

#46941

Purpose

Codes for the SSA-TLR with the target site for the CCR5 TALEN with the spacer region corresponding to I-Sce I in a lentiviral backbone

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Inserts

5' iRFP arm
eGFP with TALEN TS
+3 mCherry
3' iRFP arm

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

embedded TALEN TS from 163-218, truncated 25 amin…

Promoter

SFFV

Available since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pNB1-LOX

Plasmid

#154058

Purpose

Level 1, Position 3 Golden Gate vector. ZmUbi-5'UTR:loxP-GUS-nosT-loxP-NAM-B1-nosT

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

LoxP-flanked GUS and TtNAM-B1

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

The TtNAM-B1 gene sequence was domesticated to re…

Promoter

ZmUbi

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-Zeo-TTF-1

Plasmid

#75089

Purpose

retrovirus-mediated gene transfer into mammalian cells

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Insert

TTF-1 (NKX2-1 Human)

Use

Retroviral

Tags

none

Expression

Mammalian

Mutation

wild-type, full-length, without native 3' UTR

Promoter

Available since

May 31, 2016

Availability

Academic Institutions and Nonprofits only

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mPapaya-Keratin14-N-18

Plasmid

#56346

Purpose

Localization: Cytokeratin, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Keratin14 (KRT14 Human)

Use

Tags

mPapaya

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only