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We narrowed to 5,091 results for: codon optimized

Showing: 1411 - 1440 of 5091 results

  2. pSMH1741

    Plasmid
    #160930
    Purpose
    Expression of soluble Sc octamer (H3)
    Depositor
    Stephen Harrison
    Inserts
    HHF1
    HTA1
    HTB1
    HHT1
    Use
    Tags
    6His-TEV, N-6His-TEV, N-terminal 6His-TEV, and no…
    Expression
    Mutation
    codon-optimized for E. coli -- see provided seque…
    Promoter
    Available since
    Dec. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    160930-plasmid-map-sequence-id-318962

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  3. pSMH1742

    Plasmid
    #160929
    Purpose
    Expression of soluble Sc octamer (Cse4)
    Depositor
    Stephen Harrison
    Inserts
    HHF1
    HTA1
    HTB1
    Cse4
    Use
    Tags
    6His-TEV, N-terminal 6His-TEV, and none
    Expression
    Mutation
    codon-optimized for E. coli -- see provided seque…
    Promoter
    Available since
    Dec. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    160929-plasmid-map-sequence-id-319094

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  4. pmCellFree_KA1_SARS-COV-2 _NSP1

    Plasmid
    #169376
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP1 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP1
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169376-plasmid-map-sequence-id-335277

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  5. pEGB3 omega1 Tnos:BASTA:Pnos - P35S:hCas9:Tnos (GB3469)

    Plasmid
    #160647
    Purpose
    module containing the human codon optimized Cas9 and the BASTA selection marker
    Depositor
    Diego Orzaez
    Insert
    BASTA / Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos, P35S
    Available since
    Dec. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    160647-plasmid-map-sequence-id-319125

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  6. pHH0103 KIBRA WW domain #1

    Plasmid
    #104229
    Purpose
    Bacterial expression of WW domain #1 from KIBRA
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    KIBRA WW domain #1 (WWOX Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    104229-plasmid-map-sequence-id-204298

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  7. MYLK4

    Plasmid
    #39175
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MYLK4 (MYLK4 Human)
    Use
    Baculovirus expression
    Tags
    His Tev
    Expression
    Mutation
    N371S, codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  8. pET-29b(+)_notufA-CmEndoCOM_3C-CBD-His

    Plasmid
    #218198
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Cordyceps militaris EndoCOM
    Depositor
    Florence Pojer
    Insert
    EndoCOM
    Use
    Tags
    3C-CBD-His
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 19-315
    Promoter
    T7
    Available since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    218198-plasmid-map-sequence-id-431794

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  9. m.3047-Left TALE-G1397-N-dead-mCherry

    Plasmid
    #211339
    Purpose
    Expression of codon-optimized dead mitochondrial base editor in human cells; cell sorting; blasticidin drug selection
    Depositor
    Monkol Lek
    Insert
    m.3047-Left TALE-G1397-N-dead-mCherry
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211339-plasmid-map-sequence-id-415656

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  10. m.3075-Left TALE-G1397-N-dead-mCherry

    Plasmid
    #211340
    Purpose
    Expression of codon-optimized dead mitochondrial base editor in human cells; cell sorting; blasticidin drug selection
    Depositor
    Monkol Lek
    Insert
    m.3075-Left TALE-G1397-N-dead-mCherry
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211340-plasmid-map-sequence-id-415644

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  11. m.5147-Left TALE-G1397-N-dead-mCherry

    Plasmid
    #211341
    Purpose
    Expression of codon-optimized dead mitochondrial base editor in human cells; cell sorting; blasticidin drug selection
    Depositor
    Monkol Lek
    Insert
    m.5147-Left TALE-G1397-N-dead-mCherry
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211341-plasmid-map-sequence-id-415657

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  12. pET3-ARF6-His6(CO)

    Plasmid
    #201887
    Purpose
    Used for bacterial expression of human protein ARF6
    Depositor
    Richard Kahn
    Insert
    ARF6 (ARF6 Human)
    Use
    Tags
    6xHis (GSGSGSHHHHHH)
    Expression
    Bacterial
    Mutation
    Codon optimized for bacterial expression
    Promoter
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    201887-plasmid-map-sequence-id-403824

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  13. pET3-ARL1-His6 (CO)

    Plasmid
    #201866
    Purpose
    Used for bacterial expression of human protein ARL1
    Depositor
    Richard Kahn
    Insert
    ARL1 (ARL1 Human)
    Use
    Tags
    6xHis (GSGSGSHHHHHH)
    Expression
    Bacterial
    Mutation
    Codon optimized for bacterial expression
    Promoter
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    201866-plasmid-map-sequence-id-403817

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  14. pAAV-hPhyB621(Y276H)-P2A-m1V

    Plasmid
    #197377
    Purpose
    The AAV vector for PhyB621(Y276H) and m1V
    Depositor
    Michiyuki Matsuda
    Insert
    Phytochrome B Y276H(1-621 aa)
    Use
    AAV
    Tags
    P2A-m1V
    Expression
    Mutation
    Human codon optimized PhyB (1-621 aa), Y276H muta…
    Promoter
    CMV
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    197377-plasmid-map-sequence-id-389799

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  15. pET3-ARF3-His6(CO)

    Plasmid
    #201884
    Purpose
    Used for bacterial expression of human protein ARF3
    Depositor
    Richard Kahn
    Insert
    ARF3 (ARF3 Human)
    Use
    Tags
    6xHis (GSGSGSHHHHHH)
    Expression
    Bacterial
    Mutation
    Codon optimized for bacterial expression
    Promoter
    Available since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    201884-plasmid-map-sequence-id-402538

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  16. pHH0103 TCERG1L(ORF-2) WW domain #1

    Plasmid
    #104294
    Purpose
    Bacterial expression of WW domain #1 from TCERG1L(ORF-2)
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    TCERG1L(ORF-2) WW domain #1 (TCERG1L Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104294-plasmid-map-sequence-id-202572

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  17. pHH0103 HYPB WW domain

    Plasmid
    #104267
    Purpose
    Bacterial expression of WW domain from HYPB
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    HYPB WW domain (SETD2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104267-plasmid-map-sequence-id-203491

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  18. pHH0103 HYPC WW domain #2

    Plasmid
    #104232
    Purpose
    Bacterial expression of WW domain #2 from HYPC
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    HYPC WW domain #2 (PRPF40B Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    104232-plasmid-map-sequence-id-204196

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  19. pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

    Plasmid
    #107941
    Purpose
    Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions
    Depositor
    Benjamin Kleinstiver, Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a (E174R/S542R/K548R)
    Use
    Tags
    3x HA and NLS (nucleoplasmin)
    Expression
    Mammalian
    Mutation
    E174R, S542R and K548R
    Promoter
    CAG
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    107941-plasmid-map-sequence-id-418295

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  20. pCJ189

    Plasmid
    #162666
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162666-plasmid-map-sequence-id-321465

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  21. pCJ190

    Plasmid
    #162667
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162667-plasmid-map-sequence-id-321443

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  22. hfCas12Max

    Plasmid
    #195336
    Purpose
    vector for encoding a human codon-optimized hfCas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized hfCas12Max
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    N243R, E336R, D892R
    Promoter
    CBh, CMV, hU6
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    195336-plasmid-map-sequence-id-389250

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  23. pET21b(+)-Is-PETase-W159H-S238F

    Plasmid
    #112203
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12
    Depositor
    Gregg Beckham, Christopher Johnson
    Article
    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115
    Insert
    PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
    Use
    Tags
    6XHIS
    Expression
    Bacterial
    Mutation
    W159H, S238F
    Promoter
    N/A
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    112203-plasmid-map-sequence-id-473446

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  24. pCAG-CBE4max-SpRYc-P2A-EGFP

    Plasmid
    #208336
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYc
    Depositor
    Pranam Chatterjee
    Article
    Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:
    Insert
    human codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    Sc++ (1-1119), SpRY (1111-1368), D10A
    Promoter
    CAG
    Available since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    208336-plasmid-map-sequence-id-439176

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  25. dCas12Max-ABE

    Plasmid
    #195335
    Purpose
    vector for encoding a human codon-optimized dCas12Max-ABE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized dCas12Max-ABE
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N243R, E336R, D1049A
    Promoter
    CBh, CMV, hU6
    Available since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    195335-plasmid-map-sequence-id-383124

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  27. dCas12Max-CBE

    Plasmid
    #195337
    Purpose
    vector for encoding a human codon-optimized dCas12Max-CBE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized dCas12Max-CBE
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N243R, E336R, D1049A
    Promoter
    CBh, CMV, hU6
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    195337-plasmid-map-sequence-id-383131

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  28. pET-21b(+)-Is-PETase-W185A

    Plasmid
    #112204
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W185A mutations, codon optimized for expression in E. coli K12
    Depositor
    Gregg Beckham, Christopher Johnson
    Article
    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115
    Insert
    PETase gene from Ideonella sakaiensis 201-F6 with W185A mutation, codon optimized for expression in E. coli K12
    Use
    Tags
    6XHIS
    Expression
    Bacterial
    Mutation
    W185A
    Promoter
    N/A
    Available since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    112204-plasmid-map-sequence-id-215853

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  30. pX330-U6-(negative selection module)-Chimeric_BB-CBh-hSpCas9

    Plasmid
    #223321
    Purpose
    It contains a ccdB-CMr negative selection module flanked by BpiI (BbsI) sites that allows excluding clones without correct insertion of sgRNA. Human codon optimized Cas9 expressing plasmid.
    Depositor
    Jonathan Arias
    Article
    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do
    Inserts
    SpCas9 codon optimized for human (cas9 Synthetic, Human)
    chloramphenicol resistance protein
    CcdB
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    223321-plasmid-map-sequence-id-447743

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Showing: 1411 - 1440 of 5091 results