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KA2058_pX330A-G2P

Plasmid

#124202

Purpose

Expression of SpCas9 and sgRNA

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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p415 Gcs1

Plasmid

#112646

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Gcs1

Use

Tags

Expression

Yeast

Mutation

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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Myog'-2A-mCherryNLS-PGK-Puro

Plasmid

#69548

Purpose

Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

Depositor

Charles Gersbach

Inserts

2A-mCherry-NLS
Myogenin 5' homology arm
Myogenin 3' homology arm (Myog Mouse)

Use

Mouse Targeting

Tags

2x nuclear localization signal and PVT1-2A "…

Expression

Mutation

Silent V205V mutation in myogenin gene to prevent…

Promoter

Available since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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TRPML3-CFP

Plasmid

#18834

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML3 (Mcoln3 Mouse)

Use

Tags

CFP

Expression

Mammalian

Mutation

Q165R, F210Y

Promoter

Available since

July 14, 2008

Availability

Academic Institutions and Nonprofits only

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KA1390_pGL4-UBCmin-luc2

Plasmid

#124185

Purpose

Luciferase reporter with the UBC minimal promoter

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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TRPML3-MYC

Plasmid

#18833

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML3 (Mcoln3 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
pHCKan-yibDp-GST

Plasmid

#134592

Purpose

Low Phosphate inducible GST

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

Glutathione S-transferase

Use

Tags

none

Expression

Bacterial

Mutation

Promoter

yibD

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCR-U6-gRNA-miniCMV-TdTomato

Plasmid

#192656

Purpose

A plasmid encoding miniCMV sgRNA and miniCMV-promoter-TdTomato

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

TdTomato

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6, miniCMV

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pHCKan-yibDp-mCherry

Plasmid

#127058

Purpose

Low phosphate inducible mCherry with N-terminal linker and His tag

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

yibDp

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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TRPML2-CFP

Plasmid

#18831

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML2 (Mcoln2 Mouse)

Use

Tags

CFP

Expression

Mammalian

Mutation

Promoter

Available since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
pGGEV_4'_+nls-mCherry-Flag-_+1_BK+

Plasmid

#49313

Purpose

Golden Gate entry vector: Position 4', Flag tagged nls-mCherry with start and stop codon

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Insert

nls-mCherry-Flag

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pF3-CAG-Tol-FRT

Plasmid

#141349

Purpose

Shuttling cassette containing the Tol ORF

Depositor

Ton Schumacher

Article

Bresser et al Commun Biol. 2020 May 29;3(1):273. doi:

Insert

Tol cassette

Use

Mouse Targeting

Tags

HA

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-Nck1 (SH2 mut)

Plasmid

#45893

Depositor

Louise Larose

Article

Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

Insert

Nck-1 SH2 mutant (NCK1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

in SH2 domain (R308K) and E302K and Q333H

Promoter

CMV

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
EYFP-TRAF2

Plasmid

#111203

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Birbach et al J Biol Chem. 2002 Mar 29;277(13):10842-5

Insert

TRAF2 (TRAF2 Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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KA2897_pPB-TREtight-UBCmin-cHA-IRESH2BmKO2

Plasmid

#124176

Purpose

piggyBac-based Dox-inducible expression (moderate)

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMX Vav1 Y3F/PH* (W495L) uGFP

Plasmid

#14564

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 . 4():5.

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

PH* (W495L)

Promoter

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pMX Vav1 Y3F/CR* (C529S) uGFP

Plasmid

#14560

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 . 4():5.

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F/CR* (C529S)

Promoter

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
KA1130_pPB-TREtight-cHA-IRESH2BmCherry

Plasmid

#124173

Purpose

piggyBac-based Dox-inducible expression

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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VV247: sdChR(C138S)-TS-GCaMP6f-ER in fck

Plasmid

#58522

Purpose

Expresses sdChR(C138S) fused to GCaMP6f in mammalian cells

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

sdChR(C138S)-TS-GCaMP6f-ER

Use

Lentiviral

Tags

GCaMP6f

Expression

Mammalian

Mutation

changed Cysteine 138 to Serine

Promoter

a-CamKII

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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KA2706_eSpCas9-PGKPacdtkCh

Plasmid

#124204

Purpose

Expression of eSpCas9(1.1) and sgRNA

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge