We narrowed to 1,143 results for: fret

1. pcDNA3.1-Cerulean-Epac2(R466A)-Venus

   Plasmid

   #138199

   Purpose

   FRET-based Epac2 activation sensor with R466A mutation in Epac2 coding sequence.

   Depositor

   Jin Zhang

   Article

   Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

   Insert

   Cerulean-Epac2(R466A)-Venus (Rapgef4 Mouse, Synthetic)
   

   Tags

   Cerulean and Venus

   Expression

   Mammalian

   Mutation

   Contains arginine-to-alanine substitution at resi…

   Promoter

   CMV

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-Cerulean-Epac2(L426W)-Venus

   Plasmid

   #138200

   Purpose

   FRET-based Epac2 activation sensor with L426W mutation in Epac2 coding sequence.

   Depositor

   Jin Zhang

   Article

   Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

   Insert

   Cerulean-Epac2(L426W)-Venus (Rapgef4 Mouse, Synthetic)
   

   Tags

   Cerulean and Venus

   Expression

   Mammalian

   Mutation

   Contains leucine-to-tryptophan substitution at re…

   Promoter

   CMV

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-PKAsub-YPet-Kras

   Plasmid

   #138206

   Purpose

   Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (bimAKAR); plasma membrane targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   PKAsub-YPet-Kras
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-TORCAR

   Plasmid

   #64927

   Purpose

   FRET-based biosensor for monitoring mTORC1 kinase activity.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   TORCAR (EIF4EBP1 Human, Synthetic)
   

   Tags

   Cerulean and YPet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Lyn-ICUE3

   Plasmid

   #61623

   Purpose

   Plasma membrane-targeted FRET biosensor for monitoring cAMP

   Depositor

   Jin Zhang

   Article

   DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

   Insert

   Lyn-ICUE3 (RAPGEF3 Human)
   

   Tags

   ECFP, N-terminal targeting sequence from Lyn kina…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmTurquoise2-T2A-Venus(L68V)

   Plasmid

   #60494

   Purpose

   Produces equimolar levels of mTurquoise2 and mVenus(L68V). It can be used as a negative control for FRET.

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   mTurquoise2-T2A-mVenus(L68V)
   

   Tags

   T2A peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-endo-EKAR4

   Plasmid

   #205513

   Purpose

   Genetically encoded FRET-based biosensor for monitoring ERK kinase activity. Targeted to endosomes.

   Depositor

   Jin Zhang

   Article

   Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

   Insert

   endo-EKAR4
   

   Tags

   2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and 6xHI…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. FEMP

   Plasmid

   #191973

   Purpose

   FRET-based indicator of ER-mitochondria proximity (FEMP)

   Depositor

   Luca Scorrano

   Article

   Naon et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

   Insert

   AkapYFP-Tav2a-CFP-sac1
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cytosolic FAK biosensor

   Plasmid

   #78300

   Purpose

   FRET biosensor. Visualization of cytosolic FAK activity in live cells

   Depositor

   Yingxiao Wang

   Article

   Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

   Insert

   Cytosolic FAK biosensor
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPBbsr2-Raichu-454HRasCT

    Plasmid

    #176029

    Purpose

    A FRET biosensor for HRas activity

    Depositor

    Michiyuki Matsuda

    Article

    Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

    Insert

    Raichu-Ras-HRasCT
    

    Expression

    Mammalian

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCSIIbsr-Eevee-ROCK-NES

    Plasmid

    #209918

    Purpose

    A FRET biosensor for ROCK activity

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    Eevee-ROCK-NES
    

    Use

    Lentiviral

    Tags

    ECFP and YPet

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. PH-PLCD1_mScarlet_IRES_SYFP2_PH_N1

    Plasmid

    #85069

    Purpose

    Yellow/red FRET biosensor for monotoring plasmamembrane PtdIns(4,5)P2 levels (PIP2)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    PH-PLCD1 (PLCD1 Human)
    

    Tags

    IRES-sYFP2-PH and mScarlet

    Expression

    Mammalian

    Mutation

    PLCD aa 1–170

    Promoter

    CMV

    Available Since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDx_mScarlet-I3-NCwt-SYFP2

    Plasmid

    #189765

    Purpose

    Dual expression vector for bacteria and mammalian cells producing mScarlet-I3-NCwt-SYFP2 fusion protein for FRET analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet-I3-NCwt-SYFP2
    

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Promoter

    CMV & Rha

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-FKBP-ICUE3-NLS

    Plasmid

    #138255

    Purpose

    FRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.

    Depositor

    Jin Zhang

    Article

    Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89

    Insert

    FKBP-ICUE3-NLS
    

    Tags

    FKBP, Nuclear localization signal (NLS), cpVenus[…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCyRFP1-RhoA

    Plasmid

    #84358

    Purpose

    fusion protein of RhoA, FRET donor

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mCyRFP1-RhoA (RHOA Human, Synthetic)
    

    Tags

    mCyRFP1 N terminal fusion to RhoA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. 5HT6-YC3.60

    Plasmid

    #48339

    Purpose

    FRET reporter for Ca2+ inside primary cilia

    Depositor

    Takanari Inoue

    Article

    Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

    Insert

    5-hydroxytriptamine receptor isoform 6 (Htr6 Mouse)
    

    Tags

    YC3.60

    Expression

    Mammalian

    Available Since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pSOMA-NES

    Plasmid

    #118939

    Purpose

    Used to express the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thaliana

    Depositor

    Patrick Krysan

    Article

    Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

    Insert

    SOMA Sensor for Arabidopsis MAPK activity with Nuclear Exclusion Signal
    

    Expression

    Plant

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPB_Cdc42-TomKat-sensor-CAAX

    Plasmid

    #172473

    Purpose

    Piggybac vector for mammalian expression of TomKat (tdTomato-tdKatushka) FRET sensor for Cdc42 activity anchored to the membrane with the polybasic and CAAX motif from Kras.

    Depositor

    Sean R Collins

    Article

    Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:

    Insert

    tdKatushka2-PBD(Pak1)-EVlinker-Cdc42-tdTomato-CAAX
    

    Use

    Piggybac transposon

    Tags

    polybasic and CAAX motif from Kras, tdKatushka2, …

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPBbsr2-5102HRasCT(Picchu)

    Plasmid

    #209917

    Purpose

    A FRET biosensor for tyrosine kinase activity

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    5102HRasCT
    

    Tags

    YPet and nTurquoise-GL

    Expression

    Mammalian

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBiex PKC - Peptide 8 GFP RFP

    Plasmid

    #83564

    Purpose

    Expresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.

    Insert

    PKC alpha (PRKCA Human)
    

    Tags

    10 nm ER/K Helix, FLAG purification tag, TEV Prot…

    Expression

    Bacterial and Insect

    Mutation

    Only catalytic domain of PKC (aa 335-672)

    Promoter

    T7 Promoter

    Available Since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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