We narrowed to 1,270 results for: vent

1. pET41-FeoB-OL1del

   Plasmid

   #216741

   Purpose

   Study the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 1 (OL1) by examining the variant lacking OL1 in its interaction with exogenous peptides.

   Depositor

   Gordon Laurie

   Article

   Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi

   Insert

   feoB (PA4358 P. aeruginosa (Bacteria))
   

   Use

   Tags

   8x His

   Expression

   Bacterial

   Mutation

   Outer loop 1 (OL1) with the sequence of [INIGGALQ…

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Flag-SKP1-K142R

   Plasmid

   #213423

   Purpose

   Expresses human SKP1 K142R mutant in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

   Insert

   human Flag tagged SKP1-K142R (SKP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Flag-SKP1-97-101-5A

   Plasmid

   #213642

   Purpose

   Expresses human SKP1-97-101-5A mutant in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

   Insert

   human Flag tagged SKP1--97-101-5A (SKP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Flag-SKP1-K163R

   Plasmid

   #213424

   Purpose

   Expresses human SKP1 K163R mutant in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

   Insert

   human Flag tagged SKP1-K163R (SKP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGMC00003

   Plasmid

   #166720

   Purpose

   sgRNA against mouse Kitl

   Depositor

   Raj Chari

   Article

   Rice et al Nat Commun. 2018 Nov 30;9(1):5099. doi:

   Insert

   Kitl (Kitl Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGMC00004

   Plasmid

   #166721

   Purpose

   sgRNA against mouse Kitl

   Depositor

   Raj Chari

   Article

   Rice et al Nat Commun. 2018 Nov 30;9(1):5099. doi:

   Insert

   Kitl (Kitl Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiGuide-Neo-NCOA7g3 (BB36)

   Plasmid

   #139454

   Purpose

   Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   NCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a / U6

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. LentiGuide-Neo-NCOA7g2 (BB35)

   Plasmid

   #139453

   Purpose

   Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   NCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a / U6

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6-chiRNA_dCrkgRNA1

   Plasmid

   #131139

   Purpose

   Encodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   dCrk gRNA1 (Crk Fly)
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pU6-chiRNA_dCrkgRNA2

    Plasmid

    #131140

    Purpose

    Encodes gRNA targeting Drosophila crk locus. Cuts 99bp beyond the 3' UTR. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    dCrk gRNA1 (Crk Fly)
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV5b HA Irx5 deltaIrot

    Plasmid

    #24995

    Depositor

    Benoit Bruneau

    Article

    Costantini et al Cell. 2005 Oct 21. 123(2):347-58.

    Insert

    Irx5 (Irx5 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deletion of Iro box (aa 314-327), 19 aa upstream …

    Promoter

    Available since

    Aug. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pCMV5b HA Irx5 deltaC

    Plasmid

    #24996

    Depositor

    Benoit Bruneau

    Article

    Costantini et al Cell. 2005 Oct 21. 123(2):347-58.

    Insert

    Irx5 (Irx5 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deletes everything after Hox Domain (aa 112-177).…

    Promoter

    Available since

    Aug. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pRRLHygro-pEF1a-p53ashL344A-mKate2-splitmVenusC

    Plasmid

    #69585

    Purpose

    Expresses mutated p53-L344A tagged with mKate2 and split C-term mVenus

    Depositor

    Galit Lahav

    Article

    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

    Insert

    p53-L344A (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mKate2 and split mVenus - C term

    Expression

    Mammalian

    Mutation

    p53 L344A mutation that prevents tetramerization …

    Promoter

    EF1alpha

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-flex-taCasp3-TEVp

    Plasmid

    #45580

    Depositor

    Jim Wells, Nirao Shah

    Article

    Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

    Has Service

    AAV1 and AAV5

    Inserts

    Caspase 3 (CASP3 Human, Synthetic)
    Tobacco Etch Virus protease
    

    Use

    AAV; Adeno associated viral vector

    Tags

    Expression

    Mutation

    Linker replaced with a TEV protease cleavage site

    Promoter

    EF1a

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. SARS-CoV-2 Nucleocapsid-3xHA-IRES-mcherry

    Plasmid

    #173030

    Purpose

    To express SARS-CoV-2 Nucleocapsid protein tagged with 3xHA and in frame with mcherry (NCP-IRES-mcherry) in mammalian cells

    Depositor

    Mohamed Fareh

    Article

    Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

    Insert

    SARS-CoV-2 Nucleocapsid phosphoprotein
    

    Use

    Lentiviral

    Tags

    3xHA and IRES-mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    Gag (truncated)

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-N-Flag-NLRP3 1-90

    Plasmid

    #75137

    Purpose

    Expresses N-terminal Flag-tagged amino acids 1-90 of NLRP3 (the pyrin domain) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    fragment encoding amino acids 1-90

    Promoter

    CMV

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. FUW-hSNCA-GFP

    Plasmid

    #215489

    Purpose

    Lentiviral expression and easy visualization of alpha-synuclein in mammalian cells

    Depositor

    Ophir Shalem

    Article

    Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

    Insert

    alpha-synuclein (SNCA Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-N-Flag-ASC1

    Plasmid

    #75134

    Purpose

    Expresses N-terminal Flag-tagged ASC1 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    PYD and CARD domain containing (Pycard Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F23S (please see depositor comments below)

    Promoter

    CMV

    Available since

    Aug. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1-iSAM

    Plasmid

    #211495

    Purpose

    AAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRa

    Depositor

    Antonella Fidanza, Lesley Forrester

    Article

    Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7

    Inserts

    UniSAM insert
    TET-ON
    Neo Resistance
    Insulator
    

    Use

    CRISPR and Synthetic Biology

    Tags

    T2A-mCherry Tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a, NA, Splicing acceptor, and TREGV promoter

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. Ef1a-pspCas13b-NES-3xFLAG-T2A-BFP

    Plasmid

    #173029

    Purpose

    For expression of cytoplasmic pspCas13b protein tagged with 3xHA-T2A-BFP for gene silencing in mamallian cells.

    Depositor

    Mohamed Fareh

    Article

    Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

    Insert

    pspCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG, BFP, HIV NES, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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