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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 26,188 results for: crispr

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Showing: 9601 - 9620 of 26188 results

  1. pCMV-T7-SpCas9(LWKYQS)-P2A-EGFP (AHK34)

    Plasmid
    #223079
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized nuclease SpCas9(LWKYQS)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(LWKYQS)=D1135L, S1136W, G1218K, E1219Y, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223079-plasmid-map-sequence-id-447658

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  2. eTd-CBEa

    Plasmid
    #196603
    Purpose
    Mammalian expression of eTd-CBEa
    Depositor
    Dali Li
    Publication
    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-TadA-8e (N46L/P29A)-nSpCas9-bpNLS-P2A-UGI-bpNLS
    Tags
    6*His
    Expression
    Mammalian
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196603-plasmid-map-sequence-id-386796

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  4. pDualEX-SMN2

    Plasmid
    #214431
    Purpose
    Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the exclusion of the frame-shifting cassette exon 7
    Depositor
    Chaolin Zhang
    Publication
    Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SMN2 EXON 7 (SMN2 Human)
    Expression
    Mammalian
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    214431-plasmid-map-sequence-id-424357

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  18. pP2A_NLS_sfGFP

    Plasmid
    #63709
    Purpose
    Expressing P2A_NLS_sfGFP with homologous arm of Nanog.
    Depositor
    Stanley Qi
    Publication
    Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nanog homologous arm, p2A, NLS, sfGFP, Nanog homologous arm
    Use
    Just a donor dna with nanog homologous arm for kn…
    Tags
    sfGFP
    Available since
    April 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    63709-plasmid-map-sequence-id-107390

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  19. pSpdCas9-huTET1CD-T2A-GFP (PX458)

    Plasmid
    #129025
    Purpose
    targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Publication
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-huTET1CD, SgRNA cloning site
    Tags
    HA-Tag, NLS and T2A-EGFP
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    129025-plasmid-map-sequence-id-454365

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  20. Ccas9LacZ

    Plasmid
    #113083
    Purpose
    Enhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences.
    Depositor
    Tibor Vellai
    Publication
    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Worm
    Available since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    113083-plasmid-map-sequence-id-218959

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Showing: 9601 - 9620 of 26188 results