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pEGFP-mCCK2R-stop

Plasmid

#179321

Purpose

Express mouse cholecystokinin 2 receptor (untagged) in mammalian cells

Depositor

Christian Gruber

Article

Taghizadeh et al Sci Rep. 2022 Jun 2;12(1):9215. doi: 10.

Insert

mouse cholecystokinin2 receptor

Expression

Mammalian

Mutation

stop codon after ORF

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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px330 WAPL gRNA

Plasmid

#165595

Purpose

Insertion of WAPL degrom

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

WAPL (Wapl Mouse)

Expression

Mammalian

Mutation

NA

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_M

Plasmid

#162815

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_M

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_M

Use

Piggybac

Expression

Mammalian

Mutation

CoV2_M(Met1,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_9b

Plasmid

#162816

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_9b

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_9b

Use

Piggybac

Expression

Mammalian

Mutation

CoV2_9b(Met1,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF6

Plasmid

#162817

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF6

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF6

Use

Piggybac

Expression

Mammalian

Mutation

CoV2_ORF6(Met1,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF7b

Plasmid

#162818

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF7b

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF7b

Use

Piggybac

Expression

Mammalian

Mutation

CoV2_ORF7b(Met1,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF10

Plasmid

#162819

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF10

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF10

Use

Piggybac

Expression

Mammalian

Mutation

CoV2_ORF10(Met1,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b_alt

Plasmid

#162821

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b_alt

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF3b_alt

Use

Piggybac

Expression

Mammalian

Mutation

CoV2_ORF3b_alt(Met1,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pJW4.13

Plasmid

#131375

Purpose

expression of MBP:PPTR-2 in C. elegans

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

pptr-2 (pptr-2 Nematode)

Expression

Bacterial

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pFOSGE_CUS1C01

Plasmid

#71066

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Taf12L (Taf12L Fly)

Use

Gateway entry vector

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only
pTTSTOP2

Plasmid

#41752

Depositor

Jeff Gelles

Article

Thoresen et al Biochemistry. 2008 Sep 9;47(36):9514-21.

Insert

kinesin heavy chain (Khc Fly)

Expression

Bacterial

Mutation

residues 1-401

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only
pUASp YFP RabX1 WT

Plasmid

#37750

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

RabX1 (chrw Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Promoter

GAL4

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only
perbB2-EGFP

Plasmid

#39321

Depositor

Martin Offterdinger

Article

Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

Insert

erbB2 (ERBB2 Human)

Tags

egfp

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only

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hNICD3(3xFLAG)-pCDF1-MCS2-EF1-copGFP

Plasmid

#40640

Depositor

Brenda Lilly

Article

Zhao et al J Biol Chem. 2012 May 11;287(20):16111-2

Insert

NICD3 (NOTCH3 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin

Plasmid

#134661

Purpose

Expresses EGFP-tagged synaptic vesicle protein

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

vesicle-associated membrane protein 2 (VAMP2 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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TPC2-mCherry

Plasmid

#240468

Purpose

Red-tagged lysosomal channel

Depositor

Antony Galione

Article

Davis et al Cell Calcium. 2023 Dec;116:102801. doi:

Insert

TPC2N2 (TPCN2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Mutated aspartate 276 to lysine.

Promoter

CMV

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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N1-mSin1.2-GFP

Plasmid

#72908

Purpose

GFP-tagged mSin1 (isoform 2)

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

Target of rapamycin complex 2 subunit MAPKAP1, isoform 2 (MAPKAP1 Human)

Tags

eGFP

Expression

Mammalian

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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mCer-TG2-YFP

Plasmid

#85471

Purpose

TG2 biosensor that allows for quantitative assessment of TG2 conformational changes in live cells using FRET

Depositor

Jeffrey Keillor, Ray Truant

Article

Caron et al PLoS One. 2012;7(8):e44159. doi: 10.1371

Insert

hTG2 (TGM2 Human)

Tags

EYFP and mCerulean

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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perbB2-ECFP

Plasmid

#40268

Depositor

Martin Offterdinger

Article

Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

Insert

erbB2 (ERBB2 Human)

Tags

ecfp

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Drp1 V2 S600A

Plasmid

#46344

Depositor

David Chan

Article

Loson et al Mol Biol Cell. 2013 Jan 2.

Insert

Drp1 V2 (Dnm1l Mouse)

Expression

Mammalian

Mutation

S600A

Promoter

CMV

Available Since

July 23, 2014

Availability

Academic Institutions and Nonprofits only