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pNK041p

Plasmid

#156331

Purpose

Expresses heterologous rRNA

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

rRNA operon, orthogonal-antiRBS

Use

Synthetic Biology

Mutation

WT antiRBS engineered to orthogonal antiRBS, PS e…

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 YPet CTAG

Plasmid

#153402

Purpose

Yellow fluorescent protein CTAG codon optimised for Arabidopsis thaliana

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

Ypet CTAG

Expression

Plant

Available Since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 eYFP CTAG

Plasmid

#153396

Purpose

Yellow fluorescent protein CTAG codon optimised for Arabidopsis thaliana

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

eYFP CTAG

Expression

Plant

Available Since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pTALE-STAR-KRAB-C

Plasmid

#87540

Purpose

Destination vector for TALE-KRAB construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN; Tale-tf (repressor)

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALE-STAR-KRAB-G

Plasmid

#87541

Purpose

Destination vector for TALE-KRAB construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN; Tale-tf (repressor)

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALE-STAR-KRAB-T

Plasmid

#87538

Purpose

Destination vector for TALE-KRAB construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN; Tale-tf (repressor)

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALE-STAR-KRAB-A

Plasmid

#87539

Purpose

Destination vector for TALE-KRAB construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN; Tale-tf (repressor)

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR207-Citrine-PTS1

Plasmid

#44588

Purpose

Gateway entry vector for Citrine fluorescent protein with a peroxisome localization signal

Depositor

Yutaka Kodama

Article

Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10

Insert

Citrine-PTS1

Tags

PTS1 (peroxisomal targeting signal type 1)

Available Since

Sept. 7, 2016

Availability

Academic Institutions and Nonprofits only

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Pu.1 NGFR

Plasmid

#80132

Purpose

overexpression of Pu.1

Depositor

Ellen Rothenberg

Article

Del Real et al Development. 2013 Mar;140(6):1207-19. do

Insert

Pu.1 (Spi1 Mouse)

Use

Retroviral

Tags

IRES-Ngfr

Expression

Mammalian

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pET21a-PaaR

Plasmid

#51193

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

PaaR

Use

Synthetic Biology

Tags

6x-his

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pCri-9b

Plasmid

#61332

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag

Expression

Bacterial

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only
pCri-8b

Plasmid

#61331

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag

Expression

Bacterial

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pCri-9a

Plasmid

#61318

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag

Expression

Bacterial

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pDEST17-1ALigN

Plasmid

#20024

Depositor

Oscar Millet

Article

Tadeo et al PLoS Biol. 2009 Dec;7(12):e1000257. doi:

Insert

1A Domain of DNA Ligase N

Tags

His tag

Expression

Bacterial

Available Since

Jan. 13, 2009

Availability

Academic Institutions and Nonprofits only
p246 eSpCas9_2gRNAs_mH11

Plasmid

#164854

Purpose

gRNA vector for targeting mouse H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Stem Cell Res. 2020 Nov 5;49:102078. doi

Insert

gRNAs for targeting mouse H11 locus

Expression

Mammalian

Promoter

U6

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pREDusk-MCS

Plasmid

#188970

Purpose

For red light-repressed bacterial expression of gene of interest

Depositor

Heikki Takala

Article

Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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p6XHis-T7(P266L)

Plasmid

#174866

Purpose

Expresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.

Depositor

Anna Pyle

Article

Chillon et al Methods Enzymol. 2015;558:3-37. doi: 10.

Insert

T7 RNA polymerase

Tags

6XHis-tag

Expression

Bacterial

Mutation

P266L

Promoter

lac promoter

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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PX852

Plasmid

#62884

Purpose

C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4).

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa536-1368)

Use

CRISPR

Tags

FKBP12, NLS SV40, and NLS nucleoplasmin

Expression

Mammalian

Promoter

CBh

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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Lifeact-mCherry

Plasmid

#193300

Purpose

mammalian transient expression - expression of Lifeact-mCherry

Depositor

Klaus Hahn

Article

Herron et al Nat Commun. 2022 Jul 27;13(1):4363. doi:

Insert

Lifeact

Tags

mCherry

Expression

Mammalian

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-RARE-luciferase

Plasmid

#13458

Depositor

T. Michael Underhill

Article

Hoffman et al J Cell Biol. 2006 Jul 3. 174(1):101-13.

Insert

Retinoic Acid Receptor Response Element

Use

Luciferase

Available Since

Nov. 17, 2006

Availability

Academic Institutions and Nonprofits only

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