We narrowed to 59,215 results for: %s

1. pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18

   Plasmid

   #172316

   Purpose

   CRISPR dCas9 directly fused to a variant of bacterial DNA methyltransferase MQ1 to target CG specific methylation to the FWA locus with three guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(Q147L)_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Human NANOS3-P2A-mCherry knock-in targeting construct

   Plasmid

   #59721

   Purpose

   Gene targeting construct for human NANOS3 locus, introducing a P2A-mCherry reporter gene. Contains an frt-PGK-Neo-PA-frt cassette that can be excised.

   Depositor

   Jacob Hanna

   Article

   Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

   Insert

   P2A mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. dCBE4-gam

   Plasmid

   #124449

   Purpose

   Expresses dead Cas9 (dCas9)-CBE4-gam

   Depositor

   George Church

   Article

   Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

   Insert

   dCBE4-gam
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   Available since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-tagRFP-NLS-HA-Bxb1

   Plasmid

   #65625

   Purpose

   Vector for cotranslational expression of tagRFP-Bxb1

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   Bxb1 integrase
   

   Use

   Tags

   HA, SV40 NLS, and tagRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-FKBP-GFP-SINTBAD

   Plasmid

   #216840

   Purpose

   Stably express FKBP-GFP-tagged SINTBAD for CID induced mitophagy

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   SINTBAD (TBKBP1 Human)
   

   Use

   Lentiviral

   Tags

   FKBP-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-CRY2-mCherry-MYPT169

   Plasmid

   #178526

   Purpose

   Expresses CRY2-mCherry-MYPT (1-169 aa) in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

   Insert

   CRY2-mCherry-MYPT169
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-WPRE

   Plasmid

   #89587

   Purpose

   For nucleus-specific turboRFP labeling in single cells, pK263 should be used with pK031.

   Depositor

   Takuji Iwasato

   Article

   Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

   Insert

   nuclear localization signal (nls) turboRFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pP2A_NLS_sfGFP

   Plasmid

   #63709

   Purpose

   Expressing P2A_NLS_sfGFP with homologous arm of Nanog.

   Depositor

   Stanley Qi

   Article

   Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

   Insert

   Nanog homologous arm, p2A, NLS, sfGFP, Nanog homologous arm
   

   Use

   Just a donor dna with nanog homologous arm for kn…

   Tags

   sfGFP

   Expression

   Mutation

   Promoter

   Available since

   April 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHLP701

   Plasmid

   #203743

   Purpose

   C-terminal tagging of target proteins with 3xV5-IAA17 degron

   Depositor

   Mark Hall

   Article

   Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

   Insert

   IAA17 auxin binding domain
   

   Use

   Pcr amplification template for gene tagging

   Tags

   3xV5

   Expression

   Mutation

   Promoter

   none

   Available since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCI syn iGluu

    Plasmid

    #106122

    Purpose

    Fast indicator for synaptic glutamate imaging (synapsin promoter)

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluSnFR S72T variant
    

    Use

    Tags

    Myc and PDGFR

    Expression

    Mammalian

    Mutation

    Changed Ser 72 to Thr

    Promoter

    synapsin

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Beta-Globin-PTC39

    Plasmid

    #130701

    Purpose

    Expresses Beta-globin-PTC39 NMD reporter (It has a Premature termination codon (PTC) at 39th Amino acid)

    Depositor

    Marvin Tanenbaum

    Article

    Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

    Insert

    Beta Globin (HBB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PTC39

    Promoter

    Pcmv

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-CRY2(D387A)-mCherry

    Plasmid

    #58369

    Purpose

    Light insensitive CRY2 mutant

    Depositor

    Won Do Heo

    Article

    Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

    Insert

    CRY2[1-498] (D387A) (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    D387A

    Promoter

    CMV

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. CSIV-TRE-ASCL1

    Plasmid

    #154883

    Purpose

    Doxycycline-dependently expresses human ASCL1.

    Depositor

    Hideyuki Okano

    Article

    Nemoto et al Cells. 2020 Dec 22;10(1). pii: cells1001

    Insert

    ASCL1 (ASCL1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA FLAG TORC3

    Plasmid

    #22976

    Depositor

    Marc Montminy

    Article

    Screaton et al Cell. 2004 Oct 1. 119(1):61-74.

    Insert

    TORC3 (CRTC3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pCDNA3-myc3-FBW5

    Plasmid

    #19905

    Depositor

    Yue Xiong

    Article

    Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

    Insert

    F-box and WD repeat domain containing 5 (FBXW5 Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. gRNA_tdtomato_reporter1_SP-2x

    Plasmid

    #79370

    Purpose

    Assays activity of transcriptional activators fused to SP Cas9

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    tdtomato
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-Bmpr2ΔKinase

    Plasmid

    #163404

    Purpose

    Expresses BMPR-2 lacking the kinase domain in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The kinase domain (202-500 aa) is deleted

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-Bmpr2Δ751-813aa

    Plasmid

    #163603

    Purpose

    Expresses BMPR-2 lacking the LIMK-binding region in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The LIMK-binding region (751-813 aa) is deleted

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 1119 pBabe puroL HA FKHR AAA

    Plasmid

    #9025

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    FKHR AAA (FOXO1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T24A S256A S319A

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pSb_init containing anti SARS-CoV-2_RBD sybody Sb#16

    Plasmid

    #153524

    Purpose

    Expression of the anti SARS-CoV-2_RBD sybody Sb#16

    Depositor

    Markus Seeger

    Article

    Walter et al bioRxiv 2020.04.16.045419

    Insert

    Sb#16
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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