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pMYO127TAG-SepT

Plasmid

#34625

Depositor

Jesse Rinehart, Dieter Söll

Article

Park et al Science. 2011 Aug 26;333(6046):1151-4.

Insert

Myoglobin-127TAG

Tags

6xHIS

Expression

Bacterial

Mutation

codon-optimized + an amber stop codon was introdu…

Promoter

Ipp

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pIREShygro HA-mVenus-mAID

Plasmid

#117714

Purpose

Expression of HA-tagged mVenus fused to mAID

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

mVenus-mAID

Tags

triple HA

Expression

Mammalian

Mutation

codon optimized mAID for murine expression

Promoter

CMV

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomApple-Kan

Plasmid

#44957

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mApple with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomApple

Expression

Yeast

Available Since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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pEB1-mClover3

Plasmid

#103994

Purpose

Plasmid encoding mClover3

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mClover3

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S30R, Y39N, …

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pEB2-DsRed-Express

Plasmid

#103995

Purpose

Plasmid encoding DsRed-Express

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

DsRed-Express

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to DsRed (R2A, K5E, N6D, T21S,…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pUG-amdSYM-mak10TR

Plasmid

#75433

Purpose

Generates truncated version of MAK10 gene, which is required for dsRNA L-A propagation in S.cerevisiae

Depositor

John McCusker

Inserts

MAK10TR
ApR
amdSYM

Expression

Yeast

Mutation

N-terminal 740 bp of MAK10 ORF

Promoter

Native promoter and PTEF

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAJM.716

Plasmid

#108518

Purpose

PBAD-YFP Reporter. Ara sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PBAD-YFP

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoTagRFP657-SpHis5

Plasmid

#44846

Purpose

Yeast tagging vector to create gene fusion with yeast optimized TagRF657 with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoTagRFP657

Expression

Yeast

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaB-mCeruleanBb

Plasmid

#118235

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCerulean

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven mCerulean

Expression

Bacterial

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pKDR-Lenti-TetOn

Plasmid

#183555

Purpose

pKDR compatible TetOn lentiviral transfer plasmid

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Type

Empty backbone

Use

Lentiviral

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pNB769

Plasmid

#60138

Purpose

Red click beetle luciferase from Promega driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

CBR

Expression

Yeast

Promoter

MET17pr

Available Since

Nov. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pBabe puro MTH1

Plasmid

#21295

Depositor

Bob Weinberg

Article

Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

MTH1 (NUDT1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 29, 2009

Availability

Academic Institutions and Nonprofits only
pFA6a-ymYPET-CaURA3

Plasmid

#118460

Purpose

Yeast tagging vector to create a gene fusion with ymYPET with CaUra3 selection. *An updated version of this plasmid is available: #168056 pFA6a-link-ymYPET-URA3.

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymYPET

Expression

Yeast

Mutation

YPET A206K, F208S, E232L, N235D

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAJM.717

Plasmid

#108517

Purpose

PTet*-YFP Reporter. aTc sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PTet*-YFP

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pKI1

Plasmid

#199118

Purpose

Expresses N-terminally Hisx6-tagged, SNAP-tagged full-length RapA in E. coli cells, suitable for fluorescent labelling

Depositor

Jeff Gelles

Article

Inlow et al Proc Natl Acad Sci U S A. 2023 Jul 11;12

Insert

H6-SNAP-RapA

Tags

6xHis and SNAP-tag

Expression

Bacterial

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pSW003

Plasmid

#111249

Purpose

Broad host-range bacterial vector

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pRSETB-ro2GFP

Plasmid

#82366

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

Insert

ro2GFP

Tags

6xHIS

Expression

Bacterial

Mutation

C48S/S65T/Q80R/S147C/Q204C

Promoter

T7

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pMito.iGABASnFR.mRuby3

Plasmid

#112179

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR

Tags

mRuby3

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pKDR-CBh

Plasmid

#183557

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (non-attenuated CBh promoter)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Unattenuated

Expression

Mammalian

Promoter

CBh

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEB1-moxVenus

Plasmid

#103993

Purpose

Plasmid encoding moxVenus

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

moxVenus

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S30R, Y39N, …

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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