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pETDuet-1 Hepatitis C Virus NS3/NS4A

Plasmid

#61696

Purpose

inducible dual expression of NS3 protease along with its cofactor NS4A

Depositor

Michael Johnson

Article

Lee et al PLoS One. 2013 Oct 9;8(10):e75144. doi:

Inserts

NS4A
NS3

Tags

his

Expression

Bacterial

Promoter

T7

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA4TO-3XFLAG Halo delta 1-117 nsp1

Plasmid

#175423

Purpose

Expresses delta 1-117 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV2 delta 1-117 nsp1

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

nsp1 C-terminus

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3- SREBP-1 (A317-318; L340)

Plasmid

#204195

Purpose

Mammalian expression of SREBP-1 (Isoform 1)

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-1 (SREBF1 Human)

Tags

E-tag and T7x2

Expression

Mammalian

Mutation

Gly to Ala 317,318; Gly to Leu 340

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Teton-puro-shFDPS #2

Plasmid

#198760

Purpose

conditional knockdown of FDPS

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2018 Oct 17;50(10):137. doi

Insert

shFDPS #2 (FDPS Human)

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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huCaV2.2 (pSAD442-1)

Plasmid

#62574

Purpose

Human CaV2.2 expression in mammalian cells

Depositor

Diane Lipscombe

Article

Groen et al Hum Mol Genet. 2015 Feb 15;24(4):987-993

Insert

CACNA1B (CACNA1B Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 3, 2015

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shCcnb2

Plasmid

#162794

Purpose

Ccnb2 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Ccnb2 shRNA (Ccnb2 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shCcnb1

Plasmid

#162793

Purpose

Ccnb1 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Ccnb1 shRNA (Ccnb1 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shBirc5

Plasmid

#160949

Purpose

Birc5 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Birc5 shRNA (Birc5 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shEsco2

Plasmid

#160957

Purpose

Esco2 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Esco2 shRNA (Esco2 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shBub1

Plasmid

#160948

Purpose

Bub1 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Bub1 shRNA (Bub1 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shCdk1

Plasmid

#160953

Purpose

Cdk1 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Cdk1 shRNA (Cdk1 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shCdc20

Plasmid

#160954

Purpose

Cdc20 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Cdc20 shRNA (Cdc20 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shKif11

Plasmid

#160962

Purpose

Kif11 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Kif11 shRNA (Kif11 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shAspm

Plasmid

#160947

Purpose

Aspm shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Aspm shRNA (Aspm Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shCdkn3

Plasmid

#160955

Purpose

Cdkn3 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Cdkn3 shRNA (Cdkn3 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shCenpf

Plasmid

#160956

Purpose

Cenpf shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Cenpf shRNA (Cenpf Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1-EF1a-TagRFP-2A-tet3G

Plasmid

#167942

Purpose

Inducible NKX2-1 overexpression

Depositor

Emma Rawlins

Article

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

TTF1 (NKX2-1 Human)

Use

Lentiviral

Promoter

tetON

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1-blast-SCRAMBLE

Plasmid

#26701

Purpose

3rd gen lentiviral shRNA expression vector containing scrambled shRNA sequence. Uses blasticidin for selection

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

scramble

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3 MDM2 1-400

Plasmid

#45754

Depositor

Robert Lefkowitz

Article

Shenoy et al Science. 2001 Nov 9;294(5545):1307-13. E

Insert

MDM2 1-400 (MDM2 Human)

Expression

Mammalian

Mutation

aa 1-400, lacks catalytic RING domain

Promoter

CMV

Available Since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
pAAV-mDlx-GFP-Fishell-1

Plasmid

#83900

Purpose

GFP expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

Depositor

Gordon Fishell

Article

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

eGFP

Use

AAV

Promoter

mDlx

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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