We narrowed to 14,351 results for: NTS

1. TL1 alphaDdCBE 3_CN (right, DddA11-N)

   Plasmid

   #222003

   Purpose

   Expresses mitochondrial cytosine base editor arm in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Insert

   COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-3_CN right)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   TALE: Q231R, W232G, S233A | DddA: S1330I, A1341V,…

   Promoter

   CMV

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. TL1 alphaDdCBE 3_NC (left, DddA11-N)

   Plasmid

   #222004

   Purpose

   Expresses mitochondrial cytosine base editor arm in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Insert

   COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-3_NC left)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   TALE: Q231R, W232G, S233A | DddA: S1330I, A1341V,…

   Promoter

   CMV

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AV13_pCAG.Cas9.gRNA.NT

   Plasmid

   #199260

   Purpose

   Expression construct encoding SpCas9 nuclease and a control non-targeting guide RNA

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Inserts

   SpCas9 nuclease
   Non-targeting (NT) control gRNA
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter and RNA polymerase III promoter for …

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAV-U6+27Linear-1, PolyU(0), Hairpin Distance = 0nt

   Plasmid

   #166993

   Purpose

   Tests for the impact of a synthetic linear tract in conjunction with a hairpin motif on human polymerase III transcription from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination module:PolyU(0), Hairpin = 0 nt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. GLN, Linear-3, PolyU(1), Hairpin Distance = 0nt

   Plasmid

   #166986

   Purpose

   Tests for the impact of 1 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination module:PolyU(1), Hairpin = 0 nt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GLY, Linear-3, PolyU(4), Hairpin Distance = 0nt

   Plasmid

   #166991

   Purpose

   Tests for the impact of 4 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination module:PolyU(4), Hairpin = 0 nt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GLY, Linear-3, PolyU(1), Hairpin Distance = 0nt

   Plasmid

   #166990

   Purpose

   Tests for the impact of 1 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination module:PolyU(1), Hairpin = 0 nt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GLN, Linear-3, PolyU(4), Hairpin Distance = 0nt

   Plasmid

   #166987

   Purpose

   Tests for the impact of 4 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination module:PolyU(4), Hairpin = 0 nt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAV-U6+27Linear-2, PolyU(0), Hairpin Distance = 0nt

   Plasmid

   #166995

   Purpose

   Tests for the impact of a synthetic linear tract in conjunction with a hairpin motif on human polymerase III transcription from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination module:PolyU(0), Hairpin = 0 nt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDF-NtXNtT4aR1AtR2NtB2

    Plasmid

    #160887

    Purpose

    Expression of N. tabacum Rubisco small subunit (rbcS-T4a), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    PT7-Nt-rbcS-T4a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rbcS-T4a: T7, chaperones: T10

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Hy_pMtnA UTR delta 21-55 eGFP SV40

    Plasmid

    #132653

    Purpose

    Expresses an eGFP mRNA from the Drosophila Metallothionein A promoter that has 20 nt of the MtnA 5' UTR

    Depositor

    Jeremy Wilusz

    Article

    Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    MtnA

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Hy_pMtnA UTR delta 41-55 eGFP SV40

    Plasmid

    #132652

    Purpose

    Expresses an eGFP mRNA from the Drosophila Metallothionein A promoter that has 40 nt of the MtnA 5' UTR

    Depositor

    Jeremy Wilusz

    Article

    Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    MtnA

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC0.054

    Plasmid

    #119572

    Purpose

    Level 0 part. Promoter

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    PpetE
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    RBS* added (22 bp) upstream of ATG. Deletion of n…

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMYA5T2/pENTR3C

    Plasmid

    #97409

    Purpose

    CMYA5 (NM_153610, nt 12205 to 12279) T allele (rs10043986) in pENTR3C, Gateway Entry vector

    Depositor

    Rita Shiang

    Article

    Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

    Insert

    CMYA5 (CMYA5 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    rs10043986 (NM_153610.4:c.12188C>T)

    Promoter

    Available since

    Aug. 21, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pCRISPEY-GAL-Kan-ADE2

    Plasmid

    #232105

    Purpose

    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    ADE2 gRNA and repair template
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Repair template introduces C at nt 466 of ADE2 to…

    Promoter

    GAL7

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV6-AC 4-μHOM

    Plasmid

    #113619

    Purpose

    Reporter for Alt-EJ, variant of EJ7-GFP. Double strand breaks are induced with the 7a & 7h, or 7a & 7i sgRNAs, with CAS9. EJ using 4 nt of microhomology causes a deletion mutation and restores GFP

    Depositor

    Jeremy Stark

    Article

    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

    Insert

    4-μHOM variant of EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAL113

    Plasmid

    #22471

    Purpose

    Expresses N-terminal domains of PhyB (with Y276H mutation), fused to mCFP and HRas C terminal CAAX palmitoylation sequence. For red-light-induced binding to PIF6.

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PhyB NT (PHYB Mouse, Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    10aaLinker-mCFP-HrasCT

    Expression

    Mammalian

    Mutation

    Y276H

    Promoter

    CMV

    Available since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. EF13E

    Plasmid

    #42942

    Depositor

    Eline Luning Prak

    Article

    Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

    Insert

    EN- Human L1 element (L1RE1 Human)
    

    Use

    Tags

    EGFP (opposite strand)

    Expression

    Mammalian

    Mutation

    AgeI-BclI fragment swapped with nt 1927-3708 from…

    Promoter

    CMV MIE, L1 5'UTR

    Available since

    April 25, 2013

    Availability

    Academic Institutions and Nonprofits only

19. msfGFP-Cx43

    Plasmid

    #69024

    Purpose

    Expresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to msfGFP (FP fused to NT of connexin). CMV promoter.

    Depositor

    David Spray

    Article

    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCRISPEY-GAL-Nat-ADE2

    Plasmid

    #232103

    Purpose

    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    ADE2 gRNA and repair template
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Repair template introduces C at nt 466 of ADE2 to…

    Promoter

    GAL7

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge