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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,333 results for: Ncl

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Showing: 961 - 980 of 1333 results

  2. pMOD_B2103b

    Plasmid
    #91062
    Purpose
    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette (doesn't include the promoter) for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette (no promoter) for cloning multiple gRNA spacers with Csy4 spacers
    Use
    CRISPR
    Promoter
    CmYLCV
    Available Since
    July 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    91062-plasmid-map-sequence-id-181865

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  3. pMDC123SB-AtTAS1c-B/c

    Plasmid
    #51772
    Purpose
    Plant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor
    Depositor
    James Carrington
    Article
    Carbonell et al Plant Physiol. 2014 Mar 19.
    Insert
    AtTAS1c-B/c
    Use
    Plant expression
    Mutation
    A. thaliana TAS1c precursor sequence including a …
    Available Since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51772-plasmid-map-sequence-id-80110

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  5. pMDC32B-AtTAS1c-B/c

    Plasmid
    #51773
    Purpose
    Plant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor
    Depositor
    James Carrington
    Article
    Carbonell et al Plant Physiol. 2014 Mar 19.
    Insert
    AtTAS1c-B/c
    Use
    Plant expression
    Mutation
    A. thaliana TAS1c precursor sequence including a …
    Available Since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51773-plasmid-map-sequence-id-80112

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  6. pIL6-Nluc-hbb

    Plasmid
    #239843
    Purpose
    Template for in vitro transcription of secreted nanoluciferase, with interleukin 6 signal peptide, flanked by the human haemoglobin beta 5' and 3' UTRs.
    Depositor
    Catherine Jopling
    Article
    Smart et al Mol Ther Nucleic Acids. 2025 Jun 24;36(3
    Insert
    Secreted nanoluciferase, with interleukin 6 signal peptide, flanked by human haemoglobin beta 5' and 3' UTRs
    Use
    Luciferase
    Promoter
    T7 (included as part of insert)
    Available Since
    July 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    239843-plasmid-map-sequence-id-477827

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  7. pTS2362_Tier3(SB)-rtTA-PuroR

    Plasmid
    #169658
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven rtTA expression and PRPBSA-driven PuroR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169658-plasmid-map-sequence-id-365631

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  15. pCXLE-dCas9VPPH-T2A-GFP-shP53

    Plasmid
    #102897
    Purpose
    EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    dCas9VPPH-T2A-GFP-shP53
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available Since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102897-plasmid-map-sequence-id-194735

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  16. Keasling-2187

    Plasmid
    #100167
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Jay Keasling
    Article
    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.
    Insert
    MevT and MBIS operon
    Expression
    Bacterial
    Promoter
    lacUV5
    Available Since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    100167-plasmid-map-sequence-id-193500

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  18. pMDC32B-AtMIR390a-NbSu-2

    Plasmid
    #213400
    Purpose
    Plant expression vector (2x35S) for expressing an amiRNA against Nicotiana benthamiana SULFUR gene from AtMIR390a precursor
    Depositor
    Alberto Carbonell
    Article
    Cisneros et al Plant J. 2022 May;110(4):1166-1181. doi:
    Insert
    Arabidopsis MIR390a precursor including amiRNA/amiRNA* sequences for silencing N. benthamiana SULFUR gene
    Expression
    Plant
    Promoter
    2x35S
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    213400-plasmid-map-sequence-id-422344

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  20. JBEI-3716

    Plasmid
    #100169
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Jay Keasling
    Article
    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.
    Insert
    codon-optimized MK
    Expression
    Bacterial
    Available Since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    100169-plasmid-map-sequence-id-193493

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Showing: 961 - 980 of 1333 results