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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 25,015 results for: Nes

Showing: 961 - 980 of 25015 results

  1. Q54A

    Plasmid
    #217912
    Purpose
    A plasmid encoding the hydrogel-forming protein PXP with the Q54A coil mutation
    Depositor
    Cole DeForest
    Article
    Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d
    Insert
    PXP Q54A
    Use
    Tags
    6x His (N and C term)
    Expression
    Bacterial
    Mutation
    Glutamine at position 55 and 267 mutated to Alani…
    Promoter
    T5
    Available Since
    June 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    217912-plasmid-map-sequence-id-437013

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  2. T40A

    Plasmid
    #217911
    Purpose
    A plasmid encoding the hydrogel-forming protein PXP with the T40A coil mutation
    Depositor
    Cole DeForest
    Article
    Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d
    Insert
    PXP T40A
    Use
    Tags
    6x His (N and C term)
    Expression
    Bacterial
    Mutation
    Threonine at position 41 and 253 mutated to Alani…
    Promoter
    T5
    Available Since
    June 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    217911-plasmid-map-sequence-id-437011

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  6. pCAGGS_cyt-AKAR5

    Plasmid
    #114138
    Purpose
    Mammalian expression of the AKAR5 sensor tagged with nucleus export signal (NES) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    AKAR5 with C-terminal nuclear export signal (NES)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    114138-plasmid-map-sequence-id-215467

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  8. lentiMPH v2

    Plasmid
    #89308
    Purpose
    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Insert
    MS2-P65-HSF1_2A_Hygro (RELA Human, Synthetic)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1a
    Available Since
    March 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    89308-plasmid-map-sequence-id-425605

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  17. pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139090
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-VPR
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139090-plasmid-map-sequence-id-273886

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  19. pLentiRNAGuide_003

    Plasmid
    #192505
    Purpose
    For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat and smRNA, evopreQ1, capture sequences and GFP
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:
    Insert
    hU6-RfxCas13d-DR1-BsmBI-DR-smRNA-SapI-CS1-evopreQ1-EFS-EGFP-2A-Puro-WPRE
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hU6
    Available Since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192505-plasmid-map-sequence-id-375155

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Showing: 961 - 980 of 25015 results