Addgene

We narrowed to 10,644 results for: cat.1

Showing: 961 - 980 of 10644 results

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pL2-1 ConjEpi PtEF2bsd Pt49202tHMGRVenusThrFLAG

Plasmid

#139964

Purpose

Over-expression of N-terminal truncated version of native 3-hydroxy-3-methyl glutaryl CoA reductase

Depositor

Raffaela Abbriano

Article

Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

Insert

ConjEpi PtEF2_bsd_PttEF2 Pt49202_tHMGRVenusThrFLAG_Ptt49202

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-mu24 1-181-2xStrep

Plasmid

#138444

Purpose

Expresses truncated mu24 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

mu24

Use

Tags

Strep

Expression

Mammalian

Mutation

Amino acids 1-181

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-mu24 1-191-2xStrep

Plasmid

#138445

Purpose

Expresses truncated mu24 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

mu24

Use

Tags

Strep

Expression

Mammalian

Mutation

Amino acids 1-191

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-mu24 1-216-2xStrep

Plasmid

#138446

Purpose

Expresses truncated mu24 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

mu24

Use

Tags

Strep

Expression

Mammalian

Mutation

Amino acids 1-216

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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CFP(1-158) in pcDNAI/Amp

Plasmid

#60895

Purpose

This vector attaches CFP(1-158) to the N-terminus of a protein.

Depositor

Catherine Berlot

Article

Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

Insert

CFP(1-158)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-puro-hRAF1-shRNA-1

Plasmid

#185371

Purpose

For tetracycline-inducible mammalian expression of shRNA: CATGAGTATTTAGAGGAAGTA that targets human RAF1

Depositor

Ayaz Najafov

Insert

RAF1 (RAF1 Human)

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pPPI4-Strep-SNAP-PD-1

Plasmid

#223569

Purpose

Protein purification of human PD-1 with Twin-Strep and SNAP tags from mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

PD-1 (PDCD1 Human)

Use

Tags

Twin-Strep, SNAP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pEC3109 (pSpy1C-Plac(1)_ffluc)

Plasmid

#218514

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Tags

Expression

Bacterial

Mutation

deletion of mrfp cassette, mutagenesis of the RBS…

Promoter

Plac(1)

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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Cerulean(1-158) in pcDNAI/Amp

Plasmid

#60897

Purpose

This vector attaches Cerulean(1-158) to the N-terminus of a protein.

Depositor

Catherine Berlot

Article

Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

Insert

Cerulean(1-158)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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KIF5C(1-560)-EF(C)

Plasmid

#61675

Purpose

Non-fluorescent, truncated kinesin-1 fused to EF Hand linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-EF(C) (Kif5c Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_aa 1-420 POLI

Plasmid

#201582

Purpose

Expresses the catalytic domain (aa 1-420) of human DNA polymerase iota with a HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

DNA polymerase iota (POLI Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Expresses amino acids 1-420 of DNA polymerase iota

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_aa 1-449 POLH

Plasmid

#201583

Purpose

Expresses the catalytic domain (aa 1-449) of human DNA polymerase eta with a HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

POLH (POLH Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Expresses amino acids 1-449 of DNA polymerase eta

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_aa 1-542 POLK

Plasmid

#201585

Purpose

Expresses the N-terminal clasp domain and catalytic domain (aa 1-542) of human DNA polymerase kappa with a HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

Polymerase kappa (POLK Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Expresses amino acids 1-542 of DNA polymerase kap…

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mNudcd3 - 1

Plasmid

#198501

Purpose

lentiviral stable expression of mNudcd3 gRNA 1

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mNudcd3 (Nudcd3 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mPHB2-1

Plasmid

#198483

Purpose

lentiviral stable expression of mPHB2 gRNA 1

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mPhb2 (Phb2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMETn_2

Plasmid

#185019

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMETn

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 1-201-2xStrep

Plasmid

#138442

Purpose

Expresses truncated ORF24 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Use

Tags

Strep

Expression

Mammalian

Mutation

Amino acids 1-201

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 1-226-2xStrep

Plasmid

#138443

Purpose

Expresses truncated ORF24 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Use

Tags

Strep

Expression

Mammalian

Mutation

Amino acids 1-226

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-BcRF1 1-168-2xStrep

Plasmid

#138447

Purpose

Expresses truncated BcRF1 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

BcRF1 (BcRF1 EBV (HHV-4))

Use

Tags

Strep

Expression

Mammalian

Mutation

Amino acids 1-168

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-BcRF1 1-178-2xStrep

Plasmid

#138448

Purpose

Expresses truncated BcRF1 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

BcRF1 (BcRF1 EBV (HHV-4))

Use

Tags

Strep

Expression

Mammalian

Mutation

Amino acids 1-178

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge