We narrowed to 1,048 results for: control gRNA

Showing: 961 - 980 of 1048 results

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SAM-DNMT3A-inactive+sgAAVS1_118

Plasmid

#213171

Purpose

Vector with sgAAVS1 used as a negative control (inactive DNMT3A) for induction of global DNA methylation.

Depositor

Sefi Rosenbluh

Article

Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

Insert

sgAAVS1_118 (AAVS1 Human)

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Promoter

E1Fa and U6

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgLacZ-U6-sgGFP-hSyn1-mCherry

Plasmid

#208834

Purpose

Control plasmid expresses mCherry under the hSyn1 promoter with sgRNAs against LacZ and GFP

Depositor

Ben Emery

Article

Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

Insert

mCherry

Use

AAV and CRISPR

Expression

Bacterial and Mammalian

Promoter

hSyn1

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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AA320

Plasmid

#215952

Purpose

Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control cell surface

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

rev{U6_v1; DR_v0 [EnAs]; sgCD47_v1 [EnAs]; DR_v1 [EnAs]; sgCD47_v2 [EnAs]}

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA315

Plasmid

#215951

Purpose

Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control lethal

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

rev{U6_v1; DR_v0 [EnAs]; sgPLK1_v1 [EnAs]; DR_v1 [EnAs]; sgPLK1_v2 [EnAs]}

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA324

Plasmid

#215953

Purpose

Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control cell surface

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

rev{U6_v1; DR_v0[EnAs]; sgCD46_v4[EnAs]; DR_v1[EnAs]; sgCD47_v2[EnAs]; DR_v2[EnAs]; sgCD55_v4[EnAs];DR_v3[EnAs];sgCD81_v4[EnAs]}

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA074

Plasmid

#215933

Purpose

Fragmid fragment: (guide cassette) Tet-regulatable guide expression; positive control cell surface

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

H1-2xTetO_v1; DR_v0 [EnAs]; sgCD4_v1 [EnAs]; DR_v1 [EnAs]; sgCD4_v2 [EnAs]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA435

Plasmid

#215962

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control cell surface

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; DR_v0 [EnAs]; sgCD274_v1; DR_v1 [EnAs]; sgADGRE5_v3; DR_v2; sgCD4_v1; DR_v3; sgDPP4_v1

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA021

Plasmid

#215929

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control cell surface

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; DR_v0 [EnAs]; sgCD4_v1 [EnAs]; DR_v1 [EnAs]; sgCD4_v2 [EnAs];

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA083

Plasmid

#215934

Purpose

Fragmid fragment: (guide cassette) reverse orientation; guide expression; positive control cell surface

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

rev{U6_v1; DR_v0 [EnAs]; sgCD4_v1 [EnAs]; DR_v1 [EnAs]; sgCD4_v2 [EnAs]}

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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sgLacZ-puro

Plasmid

#199635

Purpose

sgRNA control guide targeting bacterial LacZ

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A

Use

Lentiviral

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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sgCTRL-puro

Plasmid

#199634

Purpose

sgRNA control guide targeting intergenic region

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A

Use

Lentiviral

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pMY-CD90.2-P2A-BlastDYK-Rluc_miR

Plasmid

#163341

Purpose

Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker and a DYK-tagged Blasticidin selection marker

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Insert

CD90.2 (Thy1 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pMY-CD90.2-Cd247A_miR

Plasmid

#163328

Purpose

Retroviral vector for knockdown of murine CD247A (TCR zeta) and expression of a CD90.2 surface marker under control of the LTR promoter.

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Insert

CD90.2 (Thy1 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMX-CAG-CD90.2-Cd247A_miR

Plasmid

#163326

Purpose

Retroviral vector for knockdown of murine CD247A (TCR zeta) and expression of a CD90.2 surface marker under control of the CAG promoter.

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Insert

CD90.2 (Thy1 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMX-mPGK-CD90.2-Cd247A_miR

Plasmid

#163327

Purpose

Retroviral vector for knockdown of murine CD247A (TCR zeta) and expression of a CD90.2 surface marker under control of the murine PGK promoter.

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Insert

CD90.2 (Thy1 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

mPGK

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMX-hFTH1-CD90.2-Cd247A_miR

Plasmid

#163325

Purpose

Retroviral vector for knockdown of murine CD247A (TCR zeta) and expression of a CD90.2 surface marker under control of the human FTH1 promoter.

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Insert

CD90.2 (Thy1 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

hFTH1

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMX-hPGK-CD90.2-Cd247A_miR

Plasmid

#163324

Purpose

Retroviral vector for knockdown of murine CD247A (TCR zeta) and expression of a CD90.2 surface marker under control of the human PGK promoter.

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Insert

CD90.2 (Thy1 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

hPGK

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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CBSH3+ 3m shRNA-1

Plasmid

#160067

Purpose

3-point mutation negative control for CBSH3+ shRNA-1 targeting collybistin isoforms with SH3+ domain (plasmid 160066)

Depositor

Angel de Blas

Article

George et al J Neurochem. 2021 May;157(4):1032-1051.

Insert

ARHGEF9 (Arhgef9 Rat)

Use

RNAi

Mutation

GGcTGaTTaCAACAAGGATTG (mutations shown in lower c…

Promoter

mouse U6 promoter

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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CBSH3- 3m shRNA3

Plasmid

#160213

Purpose

Knock Down MYDSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA3 targeting collybistin MYD-CBSH3- isoforms (Plasmid #160212)

Depositor

Angel de Blas

Article

George et al J Neurochem. 2021 May;157(4):1032-1051.

Insert

ARHGEF9 (Arhgef9 Rat)

Use

RNAi

Mutation

TGTATGACCTCaGGcTGtACCA (mutations shown in lower …

Promoter

mouse U6 promoter

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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CBSH3- 3m shRNA2

Plasmid

#160211

Purpose

Knock Down MTLSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA2 targeting collybistin MTL-CBSH3-isoforms (Plasmid #160210)

Depositor

Angel de Blas

Article

George et al J Neurochem. 2021 May;157(4):1032-1051.

Insert

ARHGEF9 (Arhgef9 Rat)

Use

RNAi

Mutation

TGACGTTGCTCaGGcTGtACCA (mutations shown in lower …

Promoter

mouse U6 promoter

Available Since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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