We narrowed to 25,299 results for: crispr

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AByG

Plasmid

#111083

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Publication

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Inserts

3xP3 promoter
tdTomato
Gypsy insulator
CTCF insulator
Y homology arm left
Y homology arm right

Expression

Insect

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAW204

Plasmid

#113244

Purpose

E. coli expression vector for IHFa F13A + IHFb

Depositor

Jennifer Doudna

Publication

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa F13A (ihfA E. coli)
IHFb

Expression

Bacterial

Mutation

F13A

Available since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pWD509

Plasmid

#73739

Purpose

Gateway compatible [1-2] Entry Vector encoding 2xNLS-FLP-D5

Depositor

Erik Jorgensen

Publication

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

2xNLS-FLP-D5

Expression

Worm

Mutation

G5D

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pDIV643

Plasmid

#226182

Purpose

Expresses a color marker (uidA) cassette for disruption on KU70 gene in K. phaffii.

Depositor

Uffe Mortensen

Publication

Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

Insert

uidA marker flanked with targeting regions to KU70 gene in K.phaffii

Expression

Bacterial

Promoter

TEF1p

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-E1347A-nCas9(D10A)-UGI

Plasmid

#187410

Purpose

DddA E1347A variant

Depositor

Jin-Soo Kim

Publication

Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DddA E1347A variant

Use

CRISPR

Expression

Mammalian

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-p53 gRNA-Cas9-T2A-mCherry

Plasmid

#164256

Purpose

Transcription of p53 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

Depositor

John Maciejowski

Publication

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sg p53

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLJC6-METAP1-3xFLAG

Plasmid

#163453

Purpose

lentiviral overexpression vector for human METAP1

Depositor

Jason Cantor

Publication

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

METAP1 (METAP1 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

See: comments

Promoter

Ubc

Available since

March 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-VRER

Plasmid

#159984

Purpose

Prime editing in mammalian cells

Depositor

Yongsub Kim

Publication

Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PE2-VRER

Tags

BpSV40 NLS

Expression

Mammalian

Promoter

CMV

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pUF-LbCpf1-pre-gRNA

Plasmid

#137848

Purpose

LbCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

Depositor

Josh Beck

Publication

Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LbCpf1-NLS-FLAG and gRNA cassette

Use

CRISPR

Available since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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pUF-AsCpf1-pre-gRNA

Plasmid

#137849

Purpose

AsCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

Depositor

Josh Beck

Publication

Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AsCpf1 and pre-gRNA cassette

Use

CRISPR

Available since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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EE-BE4max

Plasmid

#138158

Purpose

C-to-T base editor

Depositor

David Liu

Publication

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

rAPOBEC1(EE)-nSpCas9-UGI-UGI

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 R126E + R132E; within Cas9 D10A

Promoter

CMV

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB8748

Plasmid

#126918

Purpose

Plasmid containing integration cassette at IntE1 integration site in Yarrowia lipolytica for overexpression of XdCrtE

Depositor

Irina Borodina

Publication

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtE

Expression

Yeast

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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BCJJ339

Plasmid

#117501

Purpose

RPS5a:Cas9:E9 Golden Gate Level 1 Position 2

Depositor

Jonathan D Jones

Publication

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:GCAA:RPS5a:Cas9-3(intron):E9:ACTA:BpiI

Use

CRISPR

Expression

Plant

Promoter

AtRPS5a

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pTK372

Plasmid

#114687

Purpose

sgRNA targeting NuMA's C-terminal locus

Depositor

Tomomi Kiyomitsu

Publication

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NuMA sgRNA

Available since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-sgSmad4/Cre

Plasmid

#89651

Purpose

Expresses a Smad4-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Publication

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Smad4 (Smad4 Mouse)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pKF257

Plasmid

#52533

Purpose

expresses I-SceI nuclease in Drosophila

Depositor

Klaus Foerstemann

Publication

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

I-SceI (SCEI Budding Yeast)

Tags

HA and NLS

Expression

Insect

Promoter

tubulin

Available since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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SOX9-T2A-NLS-tdTomato-PGK-Puro

Plasmid

#89993

Purpose

Donor template for generation of SOX9-ntdTomato reporter cell lines

Depositor

Timo Otonkoski

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SOX9-T2A-NLS-TdTomato-PGK-Puro (SOX9 Human)

Use

CRISPR

Promoter

none

Available since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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ABE8e-Cas9NG

Plasmid

#203328

Purpose

Plasmid for bacterial purification of codon optimized ABE8e-Cas9NG

Depositor

Vijay Sankaran

Publication

Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

Insert

ABE8e-Cas9NG

Use

CRISPR

Tags

His-tag

Expression

Bacterial

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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ACBE

Plasmid

#204607

Purpose

Mammalian expression of adenine-to-cytosine base editor ACBE

Depositor

Dali Li

Publication

Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e-linker-C terminal of nSpCas9-bpNLS-P2A-GFP

Use

CRISPR

Tags

6*His

Expression

Mammalian

Mutation

mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)

Available since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pHnS 2comp Donor;smFP-V5 KO;Template ORF2

Plasmid

#240306

Purpose

Donor:smFP-V5 KO:Template

Depositor

Yuki Ogawa

Insert

Template gRNA

Use

AAV

Mutation

NA

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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