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pTol2-elavl3-WHaloCaMP1a-EGFP

Plasmid

#205311

Purpose

Expression of WHaloCaMP1a-EGFP in zebrafish from the elavl3 promoter (pan-neuronal expression)

Depositor

Eric Schreiter

Insert

WHaloCaMP1a-EGFP

Use

Zebrafish expression

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ332: pU6-GtFz1_omegaRNA-entry

Plasmid

#205265

Purpose

Human expression vector for GtFz1 omegaRNA (BbsI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

GtFz1 omegaRNA

Expression

Mammalian

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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PC1-29-9-2_GAL4-VHL

Plasmid

#200434

Purpose

Expresses the GAL4 DNA Binding domain linked to the VHL E3 Ubiquitin Ligase

Depositor

Xue Gao

Article

Ma et al J Am Chem Soc. 2023 Jan 25;145(3):1593-1

Insert

GAL4-VHL

Tags

GAL4, VHL

Expression

Mammalian

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1092

Plasmid

#195635

Purpose

Expression/purification of 6xHis-TEV-sfGFP

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

sfGFP

Expression

Bacterial

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHS-439

Plasmid

#195471

Purpose

Lentiviral Integratable Inducible superIL-2 cytokine using synZiFTR ZF3

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

8X ZF3 BS - ybTATA - super IL-2 - 2A -EGFP

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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BiFC1

Plasmid

#190046

Purpose

BiFC plasmid for N-terminal tagging of genes with yEmVenus (aa 1–172)

Depositor

Patrick Van Dijck

Article

Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

Insert

YemVenus (aa1-172)

Expression

Yeast

Promoter

Met3

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCBI-CRY2PHR-VPR

Plasmid

#192606

Purpose

Plasmid for constitutive expression of CRY2PHR-VPR.

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

CRY2PHR-VPR

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pEY26

Plasmid

#191031

Purpose

cho-1(3.3-2.6k) fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

cho-1(3.3-2.6k) (cho-1 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pNM262

Plasmid

#128412

Purpose

Vanillic acid inducible production of lysostaphin. LsyT with the amyE signaling peptide for secretion.

Depositor

Christopher Voigt

Article

Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

Insert

Lysostaphin (epr Staphylococcus simulans)

Use

Synthetic Biology

Tags

amyE SP

Expression

Bacterial

Promoter

Pspank(V)

Available Since

Sept. 2, 2022

Availability

Academic Institutions and Nonprofits only

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TALED_Right-stuffer-1397N

Plasmid

#187172

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Right-stuffer-1397N

Expression

Mammalian

Promoter

pCMV

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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TALED_Left-stuffer-1397C-8e

Plasmid

#187169

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Left-stuffer-1397C-8e

Expression

Mammalian

Promoter

pCMV

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19.TRIM28.FKBP.knock-in.mRuby2

Plasmid

#185008

Purpose

For knocking in FKBP F36V P2A mRuby2

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

FKBP F36V P2A mRuby2

Use

Cloning

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pE_Cre_lambdaCos

Plasmid

#173785

Purpose

The pE_Cre_lambdaCos plasmid is used to remove the antibiotic resistance cassette from pIT5 integrants.

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Insert

Cre

Use

Cre/Lox and Synthetic Biology

Mutation

S102Y and S279Y- please see depositor comments

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-N-M234I

Plasmid

#177955

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (M234I). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (M234I) (N )

Expression

Mammalian

Mutation

M234I

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-N-T205I

Plasmid

#177954

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (T205I). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (T205I) (N )

Expression

Mammalian

Mutation

T205I

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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GNb-pHuji

Plasmid

#128776

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

GNb-pHuji

Expression

Mammalian

Available Since

Feb. 9, 2020

Availability

Academic Institutions and Nonprofits only

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GNb-SNAPf

Plasmid

#128770

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

GNb-SNAPf

Expression

Mammalian

Available Since

Feb. 8, 2020

Availability

Academic Institutions and Nonprofits only

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BII-CPspaH2B

Plasmid

#133385

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

Depositor

Blair Madison

Article

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Expression

Mammalian

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pSCBE3-HF

Plasmid

#133967

Purpose

Expresses HF-BE3 CRISPR base editor and gRNA scaffold in Streptomyces

Depositor

Yuhui Sun

Article

Zhong et al bioRxiv 630137

Insert

HF-BE3

Use

CRISPR and Synthetic Biology

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pSK221

Plasmid

#129249

Purpose

reverse yTRAP, tetR-responsive Neon reporter

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

pADH1(tetO2)-mNeonGreen

Expression

Yeast

Promoter

ADH1

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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