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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,362 results for: T7

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Showing: 9801 - 9820 of 14362 results

  9. pETSUMO2_Bacteroidetes-bGSDM

    Plasmid
    #212124
    Purpose
    For expression of the bacterial gasdermin (bGSDM) encoded by a Bacteroidetes (metagenomic) species. Protein is expressed with an N-terminal SUMO2 tag.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Science. 2022 Jan 14;375(6577):221-225.
    Insert
    Bacteroidetes bGSDM
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available Since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    212124-plasmid-map-sequence-id-419109

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  15. pBiex PKC - Peptide 8 mCit mCer

    Plasmid
    #83563
    Purpose
    Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.
    Depositor
    Sivaraj Sivaramakrishnan
    Article
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PRKCA (PRKCA Human)
    Tags
    10 nm ER/K Helix, FLAG purification tag, TEV Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available Since
    Nov. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    83563-plasmid-map-sequence-id-159470

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  20. pQE80l B-CN-B

    Plasmid
    #196477
    Purpose
    Protein expression of SpyCatcher-ELP-CarHCN-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCN
    Depositor
    Fei Sun
    Article
    Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1
    Insert
    B-CN-B
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    196477-plasmid-map-sequence-id-387241

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Showing: 9801 - 9820 of 14362 results