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pX459V2.0-HypaCas9-mRuby2

Plasmid

#183872

Purpose

pX459V2.0 plasmid expressing HypaCas9 with P2A-mRuby2 and T2A-Puro, with a sgRNA cloning site for CRISPR editing in mammalian cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Type

Empty backbone

Use

CRISPR

Tags

HypaCas9-P2A-mRuby2-T2A-Puro

Expression

Mammalian

Promoter

Cbh

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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piggyBac-ancBE4-NG

Plasmid

#226865

Purpose

Expresses dox-inducible ancBE4max-NG for mammalian cells. piggyBac vector

Depositor

Alexis Komor

Article

Osgood et al Methods Enzymol. 2025;712:351-404. doi:

Insert

ancBE4max-NG

Use

Lentiviral

Promoter

Tet-On-TRE3G

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-Flag-mAIM2-EGFP

Plasmid

#51667

Purpose

Express Flag and GFP-tagged mAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Use

Retroviral

Tags

EGFP and Flag

Expression

Mammalian

Promoter

MESV

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA6 MCV LTco

Plasmid

#40200

Depositor

Patrick Moore

Article

Shuda et al J Clin Invest. 2011 Sep;121(9):3623-34.

Insert

MCPyV LT codon optimized

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-TNFalpha(Ozoralizumab) PAGER(FL)

Plasmid

#230024

Purpose

Expresses anti-TNFalpha PAGER(FL) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

pAAV-MT3-Ozoralizumab-TEVcs-ALFA-hM4DxcpGFP

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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PM PKA Reporter

Plasmid

#104551

Purpose

Expresses Lyn-mTurquoise2-VASP at the plasma membrane of mammalian cells

Depositor

David Lawrence

Article

O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

Insert

Lyn-mTurquoise2-VASP 148 - 164

Tags

mTurquoise 2, HA tag

Expression

Mammalian

Promoter

EF1 alpha

Available Since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-hAIM2-DsRed monomer

Plasmid

#51609

Purpose

Express DsRed monomer-tagged hAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Use

Retroviral

Tags

DsRed monomer

Expression

Mammalian

Promoter

MESV

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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Nanog-2A-mCherry

Plasmid

#48680

Purpose

For knock-in donor vector by CRISPR/CAS system

Depositor

Rudolf Jaenisch

Article

Yang et al Cell. 2013 Aug 27. pii: S0092-8674(13)01

Insert

Nanog (Nanog Mouse)

Use

CRISPR

Tags

Cherry

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-mCherry(LaM6) PAGER(G12)

Plasmid

#230013

Purpose

Expresses anti-mCherry PAGER(G12) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-LaM6-TEVcs-ALFA-hM1Dx12

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-mCherry(LaM6) PAGER(Gq)

Plasmid

#230010

Purpose

Expresses anti-mCherry PAGER(Gq) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-LaM6-TEVcs-ALFA-hM1D

Use

AAV

Expression

Mammalian

Mutation

W442A on hM1D

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pFLAG-ZYG11B

Plasmid

#110550

Purpose

expresses a FLAG-tagged human ZYG11B protein in mamalian cells

Depositor

Edward Kipreos

Article

Balachandran et al J Cell Biol. 2016 Oct 24;215(2):151-166.

Insert

ZYG11B (ZYG11B Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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TIGRE-TRE-[poor kozak]Bxb1-ecDHFR-BGHpA

Plasmid

#158390

Purpose

Inducible Bxb1 integrase for mammalian cells

Depositor

Michael Elowitz

Article

Chow et al Science. 2021 Apr 9;372(6538). pii: 372/

Insert

Bxb1-ecDHFR

Expression

Mammalian

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-mCherry(LaM6) PAGER(FL)

Plasmid

#230023

Purpose

Expresses anti-mCherry PAGER(FL) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

pAAV-MT3-LaM6-TEVcs-ALFA-hM4DxcpGFP

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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R26-pCAG-Ceru-10unit-BGHpA

Plasmid

#158387

Purpose

10-unit intMEMOIR recording array for mammalian cells

Depositor

Michael Elowitz

Article

Chow et al Science. 2021 Apr 9;372(6538). pii: 372/

Insert

intMEMOIR 10-unit array

Expression

Mammalian

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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Tet-CA-Src-GFP

Plasmid

#83469

Purpose

Lentiviral vector for doxycycline-inducible expression of constitutively active c-Src in mammalian cells

Depositor

Barry Gumbiner

Article

Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

Insert

Src (SRC Chicken)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Y527F (constitutively active)

Promoter

doxycycline-inducible promoter

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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EK0498 SFFV-mCherry-SGc(ACTB.s1)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#191182

Purpose

Expression of EACTB sensor in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:ACTB.s1:EGFP-3xMS2

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pKC31

Plasmid

#226777

Purpose

Expresses Drosophila UNC-104(R562H) in insect cells

Depositor

Kyoko Chiba

Article

Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

Insert

Drosophila unc-104 (R562H) (unc-104 Fly)

Tags

sfGFP and 2xStrepII

Expression

Insect

Mutation

changed Arginine 562 to Histidine (bris mutation)

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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EK0441 CMV-TO-BFPmut-stop-EGFP.t2 (FLP-IN)

Plasmid

#191161

Purpose

Expression of a trigger sequence from EGFP CDS in 3' UTR context in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

EGFP.t2

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-SpaCas12f1-gRNA_MS13

Plasmid

#190877

Purpose

The plasmid of CRISPR/SpaCas12f1(Syntrophomonas palmitatica Cas12f1) for genome editing in human cells

Depositor

Quanjiang Ji

Article

Wang et al Cell Rep. 2022 Sep 27;40(13):111418. doi

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-SH3BP4

Plasmid

#46891

Purpose

expresses HA tagged SH3BP4 in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2012 Jun 29;46(6):833-46. doi:

Insert

SH3BP4 (SH3BP4 Human)

Tags

HA

Expression

Mammalian

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only